Chemical Messengers in Insects and Plants

Hendry, Lawrence B.; Kostelc, James G.; Hindenlang, D. M.; Wichmann, J. K.; Fix, C. J.; Korzeniowski, Stephen H.

doi:10.1007/978-1-4684-2646-5_7

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The specific activity of esterase in hindgut tissue of the two ages of S. gregaria was high followed by that in midgut tissue. The esterase activity may be primarily due to carboxylesterase as mentioned by Hendry et al (1975). They stated that the role of esterase in the hindgut tissue is unclear, but since the hindgut tissue plays a role in excretion, it is conceivable that the carboxylesterase is involved in the hydrolysis of metabolically inert and undesirable esters.…”

Section: Discussionmentioning

confidence: 99%

Esterase activity and detection of carboxylesterase and phosphotriesterase in female desert locust Schistocerca gregaria (Forskal) in relation to tissues and ages

Rashad

2008

Egyptian Academic Journal of Biological Sciences. A, Entomology

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General esterases activity in the different body tissues of adult females of 2-day and 13-day-old Schistocerca gregaria (Forskal) were measured spectrophotometricaly and carboxylesterase and phosphotriesterase were characterized by electrophoresis method. Esterase activities were studied in the tissues of brain, fore-, mid-, hindgut and ovary of the two ages in the presence of α-naphthyl acetate as a substrate and ethylenediamine tetraacetic acid (EDTA) and profenofos as inhibitors. Spectrophotometric analysis indicated that the esterase activity in adult S. gregaria tissues was significantly higher in the tissues of mid-, foregut, ovary and brain of 2day-old females than those of 13-day-old, respectively. Meanwhile, the activity was greater in the hindgut tissue of 13-day-old females than in 2-day-old ones. Nondenaturing polyacrylamide gel electrophoresis revealed differences in isozyme composition. Three to ten esterase bands were detected in the different body tissues of the two ages of adult females. These bands were characterized as phosphotriesterase and carboxylesterase by using the two inhibitors. Bands Est-1, Est-2, Est-3 and Est-6 were found to be carboxylesterase in the different tissues, while band Est-4 was only detected to be phosphotriesterase.

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Section: Discussionmentioning

confidence: 99%