Chemical Microscopy

Surface-enhanced Raman and fluorescence joint analysis of soil humic acids

“…Spectroscopic imaging and micro-confocal techniques have been developed for both Raman and fluorescence spectroscopy with promising analytical applications [40], and could also be of interest in the study of HA, as demonstrated by previous studies [41]. The combination of surface-enhanced techniques and microscopy is also a powerful methodology which has attracted much attention in the analysis of biological systems such as cells or bacteria [42,43] and which could be very helpful in the characterisation of HS.…”

3D Visualization of the Dissolution Products of a Metal in the Near-Electrode Layer at the Metal-Solution Interface

“…It is worth noting that the formation of corrosion products by reaction (4) proceeds not directly at the anodic sites, but in the corrosion solution at the interface boundary with the surface corroded (Fig. 13).…”

“…It is often necessary to know the mean thickness, 3D topology (3D relief), and defectiveness of the surface layer at various surface spots. In particular, studying the 3D topology (relief) of the surface layers at a microscopic scale provides important additional information about the sources and mechanism of the metal dissolution and destruction [4]. For coordination compounds, especially informative and productive is the combination of 3D-visualization techniques with the UV, Vis, IR, and Raman spectroscopy of the mirror and diffuse reflection.…”

“…All this substantially improves the sensitivity and speed of response of the method and broadens its possibilities and the sphere of application. These methods are based on digital scanning video-microprobes [4,14] (optical scanners with a high resolution), which enable one to study in detail relatively large surface areas [15].…”

Sub-Attomole Molecule Detection in a Single Biological Cell in-vitro by Thermal Lens Microscopy

“…[1][2][3] Microscopic techniques are being extensively investigated recently. 4 Elemental analysis with electrons or ions as an excitation source is a well-established method and its spatial resolution now exceeds the nanometer region. As it needs high vacuum environment, however, it is applicable to only a limited kinds of samples.…”