Chemical modification of polystyrene's surface and its effect on immobilized antibodies

Page, Joe; Derango, R.; Huang, Ailun

doi:10.1016/s0927-7757(97)00176-3

Cited by 25 publications

(18 citation statements)

References 19 publications

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“…In practical, those hydrophilic groups are introduced by the surface treatment of ion irradiation [8], plasma [9], electron beam or UV [10,11]. Page et al modified the surface of PS beads by the nitrating process using sulfuric and nitric acids, followed by the reduction of nitro groups [12]. They confirmed the resulting amine groups were able to immobilize antibodies.…”

Section: Introductionmentioning

confidence: 99%

Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Jin

Dai

2015

Enzyme and Microbial Technology

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Section: Introductionmentioning

confidence: 99%

Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Jin

Dai

2015

Enzyme and Microbial Technology

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“…For example, silanol chemistry can be exploited for glass or silica beads. More involved immobilization protocols are required for polymer beads such as polystyrene 127 and agarose beads 128 to induce functional groups on the polymeric surfaces.…”

Section: Packed Bead Bedsmentioning

confidence: 99%

Protein immobilization techniques for microfluidic assays

2013

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Microfluidic systems have shown unequivocal performance improvements over conventional bench-top assays across a range of performance metrics. For example, specific advances have been made in reagent consumption, throughput, integration of multiple assay steps, assay automation, and multiplexing capability. For heterogeneous systems, controlled immobilization of reactants is essential for reliable, sensitive detection of analytes. In most cases, protein immobilization densities are maximized, while native activity and conformation are maintained. Immobilization methods and chemistries vary significantly depending on immobilization surface, protein properties, and specific assay goals. In this review, we present trade-offs considerations for common immobilization surface materials. We overview immobilization methods and chemistries, and discuss studies exemplar of key approaches—here with a specific emphasis on immunoassays and enzymatic reactors. Recent “smart immobilization” methods including the use of light, electrochemical, thermal, and chemical stimuli to attach and detach proteins on demand with precise spatial control are highlighted. Spatially encoded protein immobilization using DNA hybridization for multiplexed assays and reversible protein immobilization surfaces for repeatable assay are introduced as immobilization methods. We also describe multifunctional surface coatings that can perform tasks that were, until recently, relegated to multiple functional coatings. We consider the microfluidics literature from 1997 to present and close with a perspective on future approaches to protein immobilization.

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“…In order to distinguish covalent coupling from a passive absorption for the antibody, Tween 20 was used as surfactant to detach the antibody passively adsorbed on the UCN surface [22]. Tween 20 is a well-known surfactant for inducing desorption of proteins.…”

Section: Antibody Conjugationmentioning

confidence: 99%

Imaging gap junctions with silica-coated upconversion nanoparticles

Nagarajan

Marchi-Artzner

et al. 2010

Med Biol Eng Comput

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Upconversion nanoparticles (UCN) that are excited in the near infrared (NIR) region were synthesized and modified to enable their application to biological systems for imaging. The UCN obtained are oleic acid capped and hence hydrophobic in nature. Since the particles were to be used for imaging cells, a surface modification to make them hydrophilic and biocompatible was performed. Silica coating was chosen for the modification due to the possibility to further functionalize the surface and conjugate biomolecules. Cardiac cells which are capable of forming gap junctions were selected to be labeled. Gap junction specific antibodies were conjugated to the silica-coated UCN. The fluorescence emission spectrum of the particles was obtained with a continuous wave 980 nm laser and size of the particles before and after coating was determined to be 30 and 50 nm, respectively, by TEM. A covalent coupling method was used to bind the gap junction specific antibody to the nanoparticles. The fluorescence imaging experiments were carried out on cardiomyoblast cells and co-culture of bone marrow stem cells/cardiomyoblast cells after incubation with the antibody-modified UCN. Images of the particles after incubation with cardiac cells obtained over days demonstrated the potentials of the UCN for fluorescence imaging.

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Chemical modification of polystyrene's surface and its effect on immobilized antibodies

Cited by 25 publications

References 19 publications

Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Protein immobilization techniques for microfluidic assays

Imaging gap junctions with silica-coated upconversion nanoparticles

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