Chemical modification studies onCocos nucifera pollen allergens

Karmakar, P. Roy; Chatterjee, Bishnu P.

doi:10.1007/bf02720099

Cited by 3 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IEF produced two bands in C"1I (pi 3.1 and 3.5) and one band in CnVIl (pi 3.8). They have 39% common epitopes and both show highest IgE-specific reac tivity at pH 7.5 [17].…”

Section: Pollenmentioning

confidence: 99%

See 1 more Smart Citation

Placebo-Controlled Immunotherapy with <i>Cocos nucifera</i> Pollen Extract

Karmakar

Chatterjee

1994

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The effectiveness of Cocos nucifera pollen extract immunotherapy (CPE-IT) was studied in 96 patients allergic to C. nucifera pollen. A placebo-controlled study was performed at random for a period of 6–12 months. The clinical status of the patients measured by the symptom-medication scores demonstrated that C. nucifera pollen-allergic patients had significant (p < 0.005) clinical improvement after CPE-IT in comparison to placebo treatment. Serological study resulted a significant reduction (p < 0.001) of specific IgE and significant elevation (p < 0.01) of specific IgG in post-therapeutic patients’ sera which were correlated significantly (r = 0.45, p < 0.001); the changes of the above immunoglobulin levels in the placebo-treated patients were nonsignificant. However, there was no correlation between symptom-medication scores and changes in specific serum IgE or IgG levels.

show abstract

“…IEF produced two bands in C"1I (pi 3.1 and 3.5) and one band in CnVIl (pi 3.8). They have 39% common epitopes and both show highest IgE-specific reac tivity at pH 7.5 [17].…”

Section: Pollenmentioning

confidence: 99%

“…Direct IgE-ELISA and ELISA inhibition revealed that both the fractions had strong lgE-binding activity 116]. Both C"U and C"VII were found to be homogeneous by gel filtration and SDS-PAGE (12%) [ 17]. However.…”

Section: Pollenmentioning

confidence: 99%

Placebo-Controlled Immunotherapy with <i>Cocos nucifera</i> Pollen Extract

Karmakar

Chatterjee

1994

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

Isolation and characterization of two IgE-reactive proteins fromAzadirachta indica pollen

Karmakar

Chatterjee

1994

Mol Cell Biochem

View full text Add to dashboard Cite

Two allergenically active components present in the Azadirachta indica whole pollen extract have been isolated by sequential ammonium sulfate precipitation (0-90%), DEAE-Sephadex A-50 ion-exchange chromatography followed by gel filtration through Sephadex G-200. The allergenicity of fractionated materials has been tested by skin prick test and ELISA inhibition which reveal that AIaI and AIaIVb are the major allergens. Immunoblot confirms the IgE-binding activity of the proteins. Although both fractions are found to be homogeneous by SDS-PAGE, isoelectric focusing produces more than one isoelectric point in AIaI (pI = 3.15, 3.3 and 3.5) and AIaIVb (pI = 6.0 and 6.2). Amino acid analyses of the two allergens, the effect of pH on them and cross-reactivity between them have been discussed.

show abstract