Chemical mutagenesis testing in Drosophila. V. Results of 53 coded compounds tested for the national toxicology program

Abstract

Fifty-three chemicals were tested for mutagenicity in Drosophila melanogaster by adult feeding and, where results were negative, by adult injection for the induction of sex-linked recessive lethal mutations in meiotic and postmeiotic germ cell stages of Canton-S males. One compound was tested by inhalation. Those compounds that induced lethal mutations were tested further for the induction of reciprocal translocations. Seventeen of the 53 compounds (acetaldehyde, 2-aminoanthracene, bromoform, t-butyl hydroperoxide, chlorambucil, trans-cinnamaldehyde, crotonaldehyde, 1,3-dichloro-5,5-dimethylhydantoin, 3,4-dichloronitrobenzene, dimethoxane, 2,4-dinitrotoluene, 1,2-epoxy-3,3,3-trichloropropane, formaldehyde, furfural, halothane, HC yellow 4, and picric acid) were found to induce lethal mutations and three (2-aminoanthracene, chlorambucil,and crotonaldehyde) also induced reciprocal translocations.

Acetaldehyd [MAK Value Documentation in German language, 2008]

“…Differenzielle Abtötung Morita et al 1997Ma et al 1985Woodruff et al 1985 Somazellen Bei einem Drosophila-Test auf somatische Mutationen und Rekombinationen führte Acetaldehyd zu einem schwach positiven Ergebnis (Graf et al 1989). Bei einem validen Mikronukleustest am Knochenmark der Maus erwies sich Acetaldehyd (Reinheit: > 99,5%) als klastogen (Morita et al 1997).…”

“…Acetaldehyd hatte bei einem SLRL-Test an Drosophila melanogaster nach Injektion die Induktion von Letalmutationen zur Folge, nach Aufnahme über das Futter jedoch nicht (Woodruff et al 1985). Ein Mikronukleustest an frühen Spermatiden der Maus ergab ein negatives Ergebnis (Lähdetie 1988).…”

n‐Butylzinnverbindungen [MAK Value Documentation in German language, 2008]

“…DBTA (Woodruff et al 1985) und TBTO (Davis et al 1987) und verursachten in Drosophila melanogaster keine X-Chromosomen-gebundenen rezessiven Letalmutationen.…”

Flavouring Group Evaluation 66 (FGE.66):Consideration of furfuryl alcohol and related flavouring substances evaluated by JECFA (55th meeting) structurally related to Furfuryl and furan derivatives with and without additional side chain substituents and he

“…The EFSA Journal (2009) ON-752, 21-39 (Osawa & Namiki, 1982) DNA repair B. subtilis H17 (rec+) and M45 (rec-) 0.6 ml Negative a,b (Matsui et al, 1989) DNA repair B. subtilis H17 (rec+) and M45 (rec-) 1700-17 000 µg/disc Positive a,b (Shinohara et al, 1986) Forward mutation L5178Y mouse lymphoma cells, Tk+/-locus 25-100 µg/ml 200 µg/ml (Stich et al, 1981b) Chromosomal aberration Chinese hamster ovary cells 3000 µg/ml Positive (Stich et al, 1981a) Chromosomal aberration Chinese hamster ovary cells Up to 1230 µg/ml Positive a,b (NTP, 1990a) Unscheduled DNA synthesis Human liver slices 0.005-10 mmol/L Negative (Adams et al, 1998b) Sex-linked recessive lethal mutation D. melanogaster 1000 mg/kg of diet Negative (Woodruff et al, 1985) Sex-linked recessive lethal mutation D. melanogaster 100 mg/kg by injection Positive (Woodruff et al, 1985) Sex-linked recessive lethal mutation (Woodruff et al, 1985) Sister chromatid exchange Mouse bone marrow cells 50-200 mg/kg bw by injection Negative (NTP, 1990a) Spermhead abnormalities Mice 4000 mg/kg of diet daily for 5 weeks Negative (Subramanyam et al, 1989) Somatic chromosomal mutation Swiss albino mouse bone marrow cells 1000-2000 mg/kg of diet 4000 mg/kg bw for 5 days Negative Positive (Subramanyam et al, 1989) …”