Chemical nanoroughening of Ti40Nb surfaces and its effect on human mesenchymal stromal cell response

Helth, Arne; Gostin, Petre Flaviu; Oswald, Steffen; Wendrock, H.; Wolff, Ulrike; Hempel, Ute; Arnhold, Stefan; Calin, Mariana; Eckert, J.; Gebert, A.

doi:10.1002/jbm.b.32976

Cited by 45 publications

(53 citation statements)

References 48 publications

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“…The highest proliferation was observed for an Nb 2 O 5 -containing coating. Increased adhesion and spreading of human mesenchymal stromal cells on the Piranha etched surface of a Ti40Nb alloy containing an Nb 2 O 5 phase relative to the adhesion and spreading of the cells on the etched surface of Ti with the same surface topography has recently been observed [22]. In line with our previous results [4], the increased biocompatibility of the alloy surface was attributed to the presence of the surface Nb 2 O 5 phase, which strongly affects the surface energy and the charge of the alloy.…”

Section: Discussionmentioning

confidence: 85%

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

et al. 2014

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An investigation was made of the adhesion, growth and differentiation of osteoblast-like MG-63 and Saos-2 cells on titanium (Ti) and niobium (Nb) supports and on TiNb alloy with surfaces oxidized at 165°C under hydrothermal conditions and at 600°C in a stream of air. The oxidation mode and the chemical composition of the samples tuned the morphology, topography and distribution of the charge on their surfaces, which enabled us to evaluate the importance of these material characteristics in the interaction of the cells with the sample surface. Numbers of adhered MG-63 and Saos-2 cells correlated with the number of positively-charged (related with the Nb2O5 phase) and negatively-charged sites (related with the TiO2 phase) on the alloy surface. Proliferation of these cells is correlated with the presence of positively-charged (i.e. basic) sites of the Nb2O5 alloy phase, while cell differentiation is correlated with negatively-charged (acidic) sites of the TiO2 alloy phase. The number of charged sites and adhered cells was substantially higher on the alloy sample oxidized at 600°C than on the hydrothermally treated sample at 165°C. The expression values of osteoblast differentiation markers (collagen type I and osteocalcin) were higher for cells grown on the Ti samples than for those grown on the TiNb samples. This was more particularly apparent in the samples treated at 165°C. No considerable immune activation of murine macrophage-like RAW 264.7 cells on the tested samples was found. The secretion of TNF-α by these cells into the cell culture media was much lower than for either cells grown in the presence of bacterial lipopolysaccharide, or untreated control samples. Thus, oxidized Ti and TiNb are both promising materials for bone implantation; TiNb for applications where bone cell proliferation is desirable, and Ti for induction of osteogenic cell differentiation.

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Section: Discussionmentioning

confidence: 85%

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

et al. 2014

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“…Cylindrical rods of nominal compositions Ti-xNb (x = 16, 18.5, 21, 23.5, 26, 28.5, 31 wt.% Nb) 2 were prepared by casting methods in two steps [13,28]. First, button-shaped ingots were obtained by arcmelting pure Ti and Nb according to the desired composition.…”

Section: Alloy Preparationmentioning

confidence: 99%

Factors influencing the elastic moduli, reversible strains and hysteresis loops in martensitic Ti–Nb alloys

Bönisch

Calin

Humbeeck

et al. 2015

Materials Science and Engineering: C

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“…Finally, the alloy composition was determined by inductively coupled plasma optical emission spectroscopy (iCAP 6500 Duo, Thermo Fisher Scientific GmbH, Germany). The structural state of the cast and homogenized samples was checked with X-ray diffraction (STOE Stadi P instrument, STOE & CIE GmbH, Germany) and scanning electron microscopy (SEM, LEO 1530 Gemini, Carl Zeiss, Germany) and verified a polycrystalline single b-phase state with a mean grain size of approximately 70 mm [9].…”

Section: Preparation Of Titanium Alloysmentioning

confidence: 99%

Investigation of early cell–surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium–niobium alloy surfaces

et al. 2014

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Multi-potent adult mesenchymal stem cells (MSCs) derived from bone marrow have therapeutic potential for bone diseases and regenerative medicine. However, an intrinsic heterogeneity in their phenotype, which in turn results in various differentiation potentials, makes it difficult to predict the response of these cells. The aim of this study is to investigate initial cell–surface interactions of human MSCs on modified titanium alloys. Gold nanoparticles deposited on β-type Ti–40Nb alloys by block copolymer micelle nanolithography served as nanotopographical cues as well as specific binding sites for the immobilization of thiolated peptides present in several extracellular matrix proteins. MSC heterogeneity persists on polished and nanopatterned Ti–40Nb samples. However, cell heterogeneity and donor variability decreased upon functionalization of the gold nanoparticles with cyclic RGD peptides. In particular, the number of large cells significantly decreased after 24 h owing to the arrangement of cell anchorage sites, rather than peptide specificity. However, the size and number of integrin-mediated adhesion clusters increased in the presence of the integrin-binding peptide (cRGDfK) compared with the control peptide (cRADfK). These results suggest that the use of integrin ligands in defined patterns could improve MSC-material interactions, not only by regulating cell adhesion locally, but also by reducing population heterogeneity.

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Chemical nanoroughening of Ti40Nb surfaces and its effect on human mesenchymal stromal cell response

Cited by 45 publications

References 48 publications

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

Factors influencing the elastic moduli, reversible strains and hysteresis loops in martensitic Ti–Nb alloys

Investigation of early cell–surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium–niobium alloy surfaces

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