2001
DOI: 10.1016/s1367-5931(01)00259-9
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Chemical nucleases

Abstract: Substantial progress is being made in the synthesis of functional mimics of metallonuclease enzymes, although a comparison with enzyme-promoted rates of reaction shows considerable room for future improvement. Improved ligand design allows facile substrate binding to metal ion catalysts, while maintaining the stability of the metal complex. The execution of double-strand cleavage of DNA under hydrolytic conditions has been reported and new levels of activity achieved. Finally, considerable progress has been ma… Show more

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Cited by 323 publications
(70 citation statements)
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“…Studies of metal complexes, which react at specific sites along a DNA strand as reactive models for protein-nucleic acid interactions, provide routes toward rational development of chemotherapeutic agents (Lippard 1978), sensitive chemical probes for DNA structure in solution (Barton 1986), and tools for the molecular biologist to dissect genetic systems (Dervan 1986). In this regard, transition metal complexes stand out as exceptional candidates for artificial nucleases of DNA due to their diverse ability to recognize and react selectively with individual target sites (Cowan 2001;Morrow and Iranzo 2004;Tonde et al 2006). …”
Section: Introductionmentioning
confidence: 99%
“…Studies of metal complexes, which react at specific sites along a DNA strand as reactive models for protein-nucleic acid interactions, provide routes toward rational development of chemotherapeutic agents (Lippard 1978), sensitive chemical probes for DNA structure in solution (Barton 1986), and tools for the molecular biologist to dissect genetic systems (Dervan 1986). In this regard, transition metal complexes stand out as exceptional candidates for artificial nucleases of DNA due to their diverse ability to recognize and react selectively with individual target sites (Cowan 2001;Morrow and Iranzo 2004;Tonde et al 2006). …”
Section: Introductionmentioning
confidence: 99%
“…One large group of artificial nucleases are the synthetic catalytic agents, mostly metal complexes, with all their advantages and drawbacks discussed in detail elsewhere [5,[22][23][24][25]. The other class of the designed enzymes includes modified versions of native proteins constructed either by introducing mutations through molecular design or directed evolution methods or by creating chimera from independent catalytic and DNA binding domains.…”
Section: Current Artificial Nucleasesmentioning
confidence: 99%
“…The halflife of DNA under physiological conditions has not been measured but had been extrapolated to 10 6 years 39 . As a result of this high hydrolytic stability, metal nucleases would need to promote strand scission by a high magnitude in order to facilitate the reaction 40 .…”
Section: Supercoiled Dna and Small Molecule Interactionsmentioning
confidence: 99%