Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis

Mao, Sifeng; Zhang, Qiang; Liu, Wu; Huang, Qin; Khan, Mashooq; Zhang, Wanling; Lin, Caihou; Uchiyama, Katsumi; Lin, Jin‐Ming

doi:10.1039/c8sc05104f

Cited by 32 publications

(19 citation statements)

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“…Up to 10 μm diameter, up to ≈1 m in length RNA, protein, lipid Neural transmission Subcellular mRNA extraction; [33][34][35] subcellular transport [36] Positioning cells for fluidic or optical access to specific organelles and subcellular regions Subcellular transport; [33,34] automated imaging of lysosomal [31] and endosomal [69] dynamics; peroxisome formation; [32] mitochondrial trafficking and biochemical signatures within cancer cell protrusions [65,66] Microfluidic knife Lysing and introducing dye with subcellular precision ER and mitochondrial transport during wound repair [67] Micro and nanofluidic organelle traps…”

Section: Enzymatic Digestion Of Macromolecules Recyclingmentioning

confidence: 99%

“…Though this technique has the drawback of combining the material obtained from multiple cells, and thus cannot provide insight into how mRNA varies from cell to cell, it has yielded insights into how translation and modification [35] of mRNA locally within axons of developing neurons-rather than in the cell body-regulate neurodegeneration [63] and intracellular transport. [34,36] Similar studies have overcome this limitation by using manual microdissection, [64] laser manipulation, [65,66] or precisely controlled flow of lysing solution [67,68] to cut and capture protrusions from individual cells adhered in fluidic chambers. RNA sequencing and quantitative PCR of the ≈10 2 cancer cell protrusions automatically isolated in such a device has revealed a heterogeneous enrichment of mRNA associated with mitochondrial trafficking to the migrating front of these cells.…”

Section: Enzymatic Digestion Of Macromolecules Recyclingmentioning

confidence: 99%

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Micro‐ and Nano‐Devices for Studying Subcellular Biology

Siedlik

Yang²,

Kadam

et al. 2020

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Cells are complex machines whose behaviors arise from their internal collection of dynamically interacting organelles, supramolecular complexes, and cytoplasmic chemicals. The current understanding of the nature by which subcellular biology produces cell‐level behaviors is limited by the technological hurdle of measuring the large number (>103) of small‐sized (<1 μm) heterogeneous organelles and subcellular structures found within each cell. In this review, the emergence of a suite of micro‐ and nano‐technologies for studying intracellular biology on the scale of organelles is described. Devices that use microfluidic and microelectronic components for 1) extracting and isolating subcellular structures from cells and lysate; 2) analyzing the physiology of individual organelles; and 3) recreating subcellular assembly and functions in vitro, are described. The authors envision that the continued development of single organelle technologies and analyses will serve as a foundation for organelle systems biology and will allow new insight into fundamental and clinically relevant biological questions.

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Section: Enzymatic Digestion Of Macromolecules Recyclingmentioning

confidence: 99%

Section: Enzymatic Digestion Of Macromolecules Recyclingmentioning

confidence: 99%

Micro‐ and Nano‐Devices for Studying Subcellular Biology

Siedlik

Yang²,

Kadam

et al. 2020

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“…In a typical process, cell lysate was released from one micropipette and drained out from two adjacent ones, forming a local laminar flow. By positioning the laminar flow close enough to a specific area of a target cell, the area covered by the lysate can be precisely “cut off” (Mao et al, 2019 ). In addition, local operations on target portions of a living single cell could be achieved in its adherent culture state for various types of cells, and also for temporal wound repair.…”

Section: Single-cell Sampling With Nanostructuresmentioning

confidence: 99%

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Jia

Chen

2020

Front. Chem.

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The research in endogenous biomolecules from a single cell has grown rapidly in recent years since it is critical for dissecting and scrutinizing the complexity of heterogeneous tissues, especially under pathological conditions, and it is also of key importance to understand the biological processes and cellular responses to various perturbations without the limitation of population averaging. Although conventional techniques, such as micromanipulation or cell sorting methods, are already used along with subsequent molecular examinations, it remains a big challenge to develop new approaches to manipulate and directly extract small quantities of cytosol from single living cells. In this sense, nanostructure or nanomaterial may play a critical role in overcoming these challenges in cellular manipulation and extraction of very small quantities of cells, and provide a powerful alternative to conventional techniques. Since the nanostructures or nanomaterial could build channels between intracellular and extracellular components across cell membrane, through which cytosol could be pumped out and transferred to downstream analyses. In this review, we will first brief the traditional methods for single cell analyses, and then shift our focus to some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial. In this context, particular attentions will be paid to their principles, preparations, operations, superiorities and drawbacks, and meanwhile the great potential of nano-materials for single-cell sampling will also be highlighted and prospected.

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“…55 Subsequent manipulation like single-cell cutting could also be achieved by the same device. 56,57 Subsequently, a microfluidic device for mass spectrometry analysis of adherent single-cells was also developed. A single-adhered-cell was selected and…”

Section: Single-cell Manipulationmentioning

confidence: 99%

Cell Analysis on Microfluidics Combined with Mass Spectrometry

Zhang

Lin

2020

ANAL. SCI.

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Cell analysis is of great significance for the exploration of human diseases and health. However, there are not many techniques for high-throughput cell analysis in the simulated cell microenvironment. The high designability of the microfluidic chip enables multiple kinds of cells to be co-cultured on the chip, with other functions such as sample preprocessing and cell manipulation. Mass spectrometer (MS) can detect a large number of biomolecules without labelling. Therefore, microfluidic chip coupled with MS has been a major branch of cell analysis during the past decades. Here, we concisely introduce various microfluidic devices coupling with MS used for cell analysis. The main functions of microfluidic devices were first described, followed with different interfaces to different types of MS. Then, their various applications in cell analysis were highlighted, with an emphasis on cell metabolism, drug screening, and signal transduction. Current limitations and prospective trends of microfluidics coupling with MS are discussed at the end.

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Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis

Abstract: We report a laminar flow based approach that is capable of precisely cutting off or treating a portion of a single cell from its remaining portion in its original adherent state.

Cited by 32 publications

References 50 publications

Micro‐ and Nano‐Devices for Studying Subcellular Biology

Micro‐ and Nano‐Devices for Studying Subcellular Biology

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Cell Analysis on Microfluidics Combined with Mass Spectrometry

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