Chemical or enzymatic deglycosylation and germination abrogates the inhibitory activity of Cyamopsis tetragonoloba trypsin inhibitor

Patidar, Preeti; Bhayal, Mamta; Hajela, Sumati; Hajela, Krishnan

doi:10.1007/s13562-022-00777-2

Cited by 1 publication

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This property was also established with trypsin inhibitors isolated from white cranberry beans and Cyamopsis tetragonoloba seeds in the presence of CaCl 2 and glycerol as additives stabilizers. 23 , 24 According to Bijina et al, 10 the enhancement of thermal stability with CaCl 2 can be related to the ability of calcium ions to stabilize proteins through specific and nonspecific bindings within the protein molecule, preventing its unfolding at high temperatures. 10 …”

Section: Resultsmentioning

confidence: 99%

Novel Stable Protease Inhibitor from Phoenix dactylifera (L.) Flowers with Antimicrobial and Antitumoral Activities

Jemel,

Krayem,

Ben Bacha

et al. 2024

ACS Omega

View full text Add to dashboard Cite

A novel protease inhibitor isolated from date palm Phoenix dactylifera (L.) flowers (PIDF) was purified and characterized. A heat and acidic treatment step followed by ethanol precipitation and reverse-phase highperformance chromatography was applied to purify this natural protease inhibitor to homogeneity with a single band of about 19 kDa. The stability study depicted that PIDF was fully stable at 40 °C and retained 65% of its initial activity after heating at 50 °C for 24 h. Its thermal stability at 70 °C was markedly enhanced by adding calcium, bovine serum albumin, and sorbitol as well as by metal divalent cations, especially Mg 2+ and Hg 2+ . This protease inhibitor showed high inhibitory activity against therapeutic proteases, including pepsin, trypsin, chymotrypsin, and collagenase, and acted as a potent inhibitor of some commercial microbial proteases from Aspergillus oryzae, Bacillus. sp, and Bacillus licheniformis. Moreover, a potent antibacterial spectrum against Gram (+) and Gram (−) bacterial strains and an efficient antifungal effect were observed. Its cytotoxicity toward human colorectal cancer cell LoVo and HCT-116 lines suggested that PIDF could serve as a new therapeutic target inhibiting human colorectal cancer.

show abstract