Chemical Oxidation of <i>p</i>-Hydroxyphenylpyruvic Acid in Aqueous Solution by Capillary Electrophoresis with an Electrochemiluminescence Detection System

Chen, Guonan; Chi, Yuwu; Wu, Xiaoping; Duan, Jianping; Nian-bin, LI

doi:10.1021/ac034451o

Cited by 44 publications

(26 citation statements)

References 19 publications

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“…[4][5][6][7][8][9][10][11][12][13] McCall et al reported on the inhibition of Ru(bpy)3 2+ /TPrA ECL by phenol, substituted phenols, hyroquinones, catechols, and benzoquinones, and suggested a quenching mechanism involving benzoquinone derivatives formed at the electrode surface that quenches ECL via energy transfer. Li et al 12,13 observed the ECL of Ru(bpy)3…”

Section: +mentioning

confidence: 99%

Quenching of the Electrochemiluminescence of Tris(2,2′-bipyridine)-ruthenium(II) by Sodium Diphenylamine-4-sulfonate

Zhu

Zhao

et al. 2009

ANAL. SCI.

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Efficient quenching electrochemiluminescence (ECL) of the tris(2,2′-bipyridine)ruthenium(II)/tripropylamine (Ru(bpy)3 2+ /TPrA) system by a novel quencher, sodium diphenylamine-4-sulfonate (SDS), has been investigated. The quenching behaviors can be observed with a 100-fold excess of SDS over Ru(bpy)3 2+ . The mechanism of quenching is believed to involve energy transfer from the excited-state Ru(bpy)3 2+ * to the dimer of SDS, which was formed after the electrochemical oxidation of SDS at the electrode surface. Photoluminescence experiments coupled with bulk electrolysis support formation of the SDS dimer upon electrochemical oxidation.

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Section: +mentioning

confidence: 99%

Quenching of the Electrochemiluminescence of Tris(2,2′-bipyridine)-ruthenium(II) by Sodium Diphenylamine-4-sulfonate

Zhu

Zhao

et al. 2009

ANAL. SCI.

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“…ECL using tris(2,2 0 -bipyridyl)ruthenium(II) (Ru(bpy) 3 21 ) has been extensively applied for the analysis of various compounds containing secondary or tertiary amine [21,22]. In recent years, ECL has attracted increasing interest as CE end-column detector for separation and detection of amine containing compounds and their derivatives for its high sensitivity, simple instrument and low assay cost [23][24][25][26][27][28][29][30][31]. Using an end-column electrode to convert Ru(bpy) 3 21 to active Ru(bpy) 3 31 form allows a simple and sensitive ECL detection scheme.…”

Section: Introductionmentioning

confidence: 99%

CE‐ECL detection of gatifloxacin in biological fluid after clean‐up using SPE

Wang

et al. 2009

J of Separation Science

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A simple, rapid and sensitive CE method has been proposed for the determination of gatifloxacin in biological fluid. This method is based on the ECL reaction of gatifloxacin and tris(2,2'-bipyridyl)ruthenium(II) occurred in the end-column detection cell. Under the optimal conditions, gatifloxacin can be assayed within 10 min over the concentration range of 5.0 Â 10 À8 À5.0 Â 10 À6 g/mL with the theoretical plate numbers of 18 000. The intra-day and inter-day precision of the signal intensity and the migration time shows acceptable reproducibility for the analysis of gatifloxacin. The presented method has been successfully applied to determine the concentrations of gatifloxacin in urine and blood samples after clean-up using C 18 SPE column. IntroductionGatifloxacin, a synthetic broad-spectrum antimicrobial agent that is active against both gram-negative and gram-positive bacteria, is widely used for the treatment of various infections. This drug offers several advantages over previous antibiotics, including enhanced in vitro activity against clinically important pathogens and improved pharmacokinetics and pharmacodynamics characteristics, which may improve patient outcomes against certain bacterial pathogens, especially penicillin-resistant Streptococcus pneumoniae. The absolute bioavailability of gatifloxacin is 96%, with mean peak plasma concentration of 3.1-3.6 mg/mL usually occurring 1-2 h after the ingestion of a 400 mg therapeutic dosage [1][2][3].So far, some methods such as HPLC-UV [4][5][6][7], HPLCfluorimetry [6,8] [20] have been developed for the analysis of gatifloxacin in pharmaceutical preparation, biological fluid and animal tissue. Among these, HPLC [4,6,8,9] based strategies have been most universally used for the determination of this drug in biological samples since the complex matrix may severely interfere in the analysis if effective separation process is absent. High separation efficiency, low sample amount required and short run time make CE a significant complement to HPLC for the detection of various drugs and metabolites in complex sample matrix. Furthermore, as a ''green'' technique, CE separation is commonly performed in aqueous running buffers or aqueous running buffers containing a spot of organic solvent instead of organic mobile phase in HPLC, which diminishes pollution to the environment and damage to the operator. However, the sensitivity of the reported works concerning the determination of gatifloxacin in biological sample using CE is limited due to the UV detector commonly used, in which LOQ of 5.0 Â 10 À6 and 5.0 Â 10 À7 g/mL [10][11][12] are achieved. ECL using tris(2,2 0 -bipyridyl)ruthenium(II) (Ru(bpy) 3 21 ) has been extensively applied for the analysis of various compounds containing secondary or tertiary amine [21,22]. In recent years, ECL has attracted increasing interest as CE end-column detector for separation and detection of amine containing compounds and their derivatives for its high sensitivity, simple instrument and low assay cost [23][24][25][26][27][28][29...

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“…Because of the physiological and clinical significance, chemistry of pHPP has been a topic of constant interest. The methods for determination of pHPP included flow injection spectrophotometry combined with single chip microcomputer [3], fluorescence quenching [4], GC [5][6], HPLC [7], CE [8][9][10], and electrochemical technique [1]. The Keto-enol tautomerism of pHPP was early investigated by Chi et al, using electrochemical method and UV spectrometry [1].…”

Section: Introductionmentioning

confidence: 99%

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Huang¹,

Zhang²,

LiangJun³

et al. 2009

J of Separation Science

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A high-performance CE with amperometric detection (CE-AD) was employed for the kinetic study of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP). Several factors (concentration of b-cyclodextrin (b-CD), concentration and pH of running buffer, separation voltage and injection time) affecting CE-AD were investigated and separation conditions were optimized. The kinetics of pHPP was performed in water solution and phosphate solution under different pH and temperature, the homologous ketonization rate constants and half-life were obtained. Also, the activation energy was calculated according to the rate constants under different temperature. The experimental results indicated that b-CD played an important role in the separation, therefore UV spectrometric method was applied for the study of complexation interaction between ketonic pHPP and b-CD. The results indicated that the stoichiometric ratio of the pHPP-b-CD complex was 1:1 and formation constant was determined. The obtained kinetic results are in correspondence with those reported by our group with CE-UV.

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Chemical Oxidation of p-Hydroxyphenylpyruvic Acid in Aqueous Solution by Capillary Electrophoresis with an Electrochemiluminescence Detection System

Cited by 44 publications

References 19 publications

Quenching of the Electrochemiluminescence of Tris(2,2′-bipyridine)-ruthenium(II) by Sodium Diphenylamine-4-sulfonate

Quenching of the Electrochemiluminescence of Tris(2,2′-bipyridine)-ruthenium(II) by Sodium Diphenylamine-4-sulfonate

CE‐ECL detection of gatifloxacin in biological fluid after clean‐up using SPE

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

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