Chemical pretreatment of lignocellulosic wastes for cellulase production by Aspergillus niger FNU 6018

Gunam, Ida Bagus Wayan; Antara, Nyoman Semadi; Anggreni, Anak Agung Made Dewi; Setiyo, Yohanes; Wiguna, I. Putu Eka; Wijaya, I Made Mahaputra; Putra, I. W. W. P.

doi:10.1063/1.5125544

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…Por otro lado, la producción de celulasas por parte del género Aspergillus ha sido estudiada principalmente para la especie A. niger y poco reportada para A. fumigatus, no obstante, en este estudio se confirmó la capacidad que tiene un aislado nativo de esta última especie para producir enzimas celulolíticas a partir de CMC, en concentraciones superiores a las reportadas por Gunam et al (2019) quienes al cultivar A. niger con paja de maíz como sustrato lograron una actividad enzimática equivalente a la liberación de 1,1 g/L de azúcares reductores después de 7 días de cultivo.…”

Section: Confirmación De La Actividad Celulolíticaunclassified

Producción de celulasas con cultivos puros y mixtos de Trichoderma reesei y Aspergillus fumigatus usando cascarilla de arroz como sustrato

Osorio Echeverri,

Mora López,

Obando García

et al. 2023

reveia

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Las enzimas celulolíticas son usadas en diferentes industrias; muchas de ellas sonobtenidas a partir de hongos y bacterias que usan como sustrato residuos agroindustrialescon alto contenido de celulosa. Algunos hongos de los géneros Trichoderma y Aspergillussintetizan celulasas con diferentes mecanismos de acción y se han propuesto cultivosmixtos de estos hongos como estrategia para mejorar la hidrólisis de los materialescelulósicos asociada con la producción de estas enzimas. El objetivo de este estudiofue verificar la producción de celulasas a partir de cultivos mixtos y puros de aisladosnativos de T. reesei y A. fumigatus con actividad celulolítica usando cascarilla de arrozcomo sustrato. Ambos hongos producen enzimas que catalizan la liberación de azúcaresreductores a partir de carboximetilcelulosa. La fermentación en estado sólido se realizósiguiendo un diseño factorial y se analizó el efecto del tipo de inóculo y del tiempo deincubación sobre la actividad celulolítica de los extractos obtenidos. La producción decelulasas después de 15 días de incubación fue superior a la obtenida con 8 días de cultivoy se confirmó a través de un análisis de varianza que la producción de celulasas paralos cultivos mixto y puro de T. reesei en el día 15 (34,5 y 31,9 U/g respectivamente) nopresentaron diferencias significativas. Se demostró en este trabajo que la cascarilla dearroz tiene potencial para su uso en la obtención de enzimas fúngicas, aunque la actividadenzimática obtenida usando este subproducto es inferior a la producida con otrosmateriales celulósicos.

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Section: Confirmación De La Actividad Celulolíticaunclassified

Producción de celulasas con cultivos puros y mixtos de Trichoderma reesei y Aspergillus fumigatus usando cascarilla de arroz como sustrato

Osorio Echeverri,

Mora López,

Obando García

et al. 2023

reveia

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“…The supernatant was discarded, and the cells obtained were collected and combined to check the value of their optical density (OD660). After the OD660 had been known, it was equated by adding saline solution to have an OD660 value of 5, and the microbial cells were ready for use [17], [18].…”

Section: Preparation Of Microbial Culturementioning

confidence: 99%

The Effect of Mixed Inoculum Addition Concentration and Fermentation Time on the Characteristics of Dry Cocoa Beans (Theobroma cacao L.)

Gunam

Suryanto

Putra

et al. 2021

Int. J. Adv. Sci. Eng. Inf. Technol.

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The fermentation process has an important role in determining the quality of cocoa beans by forming taste, color, aroma, and appearance. The purpose of this study was to determine the effect of inoculum concentration and fermentation time on the characteristics of dry cocoa beans and determine the best conditions for both inoculum addition concentration and fermentation time to produce dry cocoa beans under Indonesian national standards. The research used completely randomized design with two factors; the first factor was the starter concentration consisting of controls (0%), 0.5%, 1%, 1.5%, and 2% (v/w). The second factor is the fermentation time, namely 1 day, 2 days, 3 days, 4 days, and 5 days. Treatment was repeated twice to get 50 units of the experiment. The results showed that the addition concentration of mixed inoculum (Saccharomyces cerevisiae and Lactobacillus plantarum) had a significant effect on changes in temperature and pH during fermentation, and it also had a significant effect on total unfermented beans. This study showed that time fermentation treatment had a very significant effect on all tests. The interaction of the two treatments had a significant effect on temperature changes during fermentation; it also significantly affected total unfermented beans. According to the Indonesian National Standard of cocoa beans, the best treatment, and the fastest fermentation time to produce the first quality standard (cut test) is a concentration of 1% mixed inoculum and fermentation in 3 days.

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“…Aspergillus niger is known to produce cellulases, including endoglucanases, exoglucanases, and β-glucosidases, which are essential for the breakdown of cellulose into glucose [17]. Enzymatic hydrolysis, particularly using Aspergillus niger, is chosen for its environmental friendliness and efficiency [18] [19].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic hydrolysis of cellulose banana stem (alkaline microwave-assisted pre-treatment)

Samara,

Novia,

Melwita

2024

JIAE

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The banana stem waste holds immense promise as a readily available and abundant source of lignocellulosic biomass, making it a compelling alternative for biofuel and biochemical applications. Therefore, this study focuses on investigating the impact of both time and substrate loading on the enzymatic hydrolysis of banana stem cellulose that has undergone alkaline microwave-assisted pre-treatment. The pre-treatment method involves subjecting the biomass to 5% KOH for 30 min, followed by microwave exposure at 300 W for 5 min, a process aimed at enhancing the accessibility of cellulose. Enzymatic hydrolysis experiments were carried out utilizing cellulase enzymes derived from Aspergillus niger, with variations in hydrolysis times (ranging from 5 to 45 h) and enzyme-to-substrate ratios (ranging from 1:1 to 1:10). The results of this investigation revealed a substantial improvement in hydrolysis efficiency, owing to the synergistic effects of alkaline microwave-assisted pre-treatment, signifying enhanced cellulose accessibility. Notably, the highest concentration of reducing sugars (1.3 mg mL−1) was achieved at a substrate-to-enzyme ratio of 1:1 and a hydrolysis duration of 45 h. These findings provide valuable insights into the conversion of lignocellulosic biomass, emphasizing the potential of integrated pre-treatment strategies for sustainable biorefinery applications. This research contributes to advancing our understanding of lignocellulosic biomass utilization, offering a promising avenue for biofuel and biochemical production from banana stem waste.

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Chemical pretreatment of lignocellulosic wastes for cellulase production by Aspergillus niger FNU 6018

Cited by 9 publications

References 18 publications

Producción de celulasas con cultivos puros y mixtos de Trichoderma reesei y Aspergillus fumigatus usando cascarilla de arroz como sustrato

Producción de celulasas con cultivos puros y mixtos de Trichoderma reesei y Aspergillus fumigatus usando cascarilla de arroz como sustrato

The Effect of Mixed Inoculum Addition Concentration and Fermentation Time on the Characteristics of Dry Cocoa Beans (Theobroma cacao L.)

Enzymatic hydrolysis of cellulose banana stem (alkaline microwave-assisted pre-treatment)

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