Chemical probe of AHL modulators on quorum sensing in Gram-Negative Bacteria and as antiproliferative agents: A review

Ampomah-Wireko, Maxwell; Luo, Chunying; Cao, Yaquan; Wang, Huanhuan; Nininahazwe, Lauraine; Wu, Chunli

doi:10.1016/j.ejmech.2021.113864

Cited by 25 publications

(10 citation statements)

References 63 publications

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“…LasI, an AHL synthesizing biological macromolecule of P. aeruginosa , was named after the induction of the elastase enzyme, which is a potent virulence factor in P. aeruginosa [ 17 ]. LasI produces 3-oxo-C12-HSL during the early development phases of P. aeruginosa growth.…”

Section: Ahl Signalmentioning

confidence: 99%

“…Once the AHL molecules are generated, they can be transported passively and actively in and out of the cells [ 15 ]. The second component of AHL signaling is the LuxR-type AHL receptor proteins, which bind to the AHL signal molecule and govern the QS-dependent activation, repression, and depression of target genes [ 16 , 17 ]. The LuxR-type DNA-binding transcription regulators govern QS-dependent gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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Microbial biodiversity includes biotic and abiotic components that support all life forms by adapting to environmental conditions. Climate change, pollution, human activity, and natural calamities affect microbial biodiversity. Microbes have diverse growth conditions, physiology, and metabolism. Bacteria use signaling systems such as quorum sensing (QS) to regulate cellular interactions via small chemical signaling molecules which also help with adaptation under undesirable survival conditions. Proteobacteria use acyl-homoserine lactone (AHL) molecules as autoinducers to sense population density and modulate gene expression. The LuxI-type enzymes synthesize AHL molecules, while the LuxR-type proteins (AHL transcriptional regulators) bind to AHLs to regulate QS-dependent gene expression. Diverse AHLs have been identified, and the diversity extends to AHL synthases and AHL receptors. This review comprehensively explains the molecular diversity of AHL signaling components of Pseudomonas aeruginosa, Chromobacterium violaceum, Agrobacterium tumefaciens, and Escherichia coli. The regulatory mechanism of AHL signaling is also highlighted in this review, which adds to the current understanding of AHL signaling in Gram-negative bacteria. We summarize molecular diversity among well-studied QS systems and recent advances in the role of QS proteins in bacterial cellular signaling pathways. This review describes AHL-dependent QS details in bacteria that can be employed to understand their features, improve environmental adaptation, and develop broad biomolecule-based biotechnological applications.

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Section: Ahl Signalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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“…Biology 2022, 11, x FOR PEER REVIEW 3 of 20 multitude (from dozen to hundreds) of genes that aid critical physiological processes [4,12]. Four main mechanisms of quorum quenching can be highlighted: (1) the inhibition of signal molecule synthesis, (2) inactivation or enzymatic degradation of signal molecules, (3) competition with signal molecules-receptor analogues, and (4) prevention of the signal transduction cascades [13].…”

Section: Introductionmentioning

confidence: 99%

“…In Gram-negative bacteria, the autoinducer molecules are acyl-homoserine lactones (AHLs) or other molecules that are produced from the enzymatic cofactor S-adenosylmethionine (SAM), whereas the autoinducers in Gram-positive bacteria are peptides [ 10 , 11 ]. Autoinducers freely pass the bacterial membrane and the process continues with them attaching themselves to their specific membrane or cytoplasmic receptor, initiating a change in gene expression in a multitude (from dozen to hundreds) of genes that aid critical physiological processes [ 4 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…The fact that quorum-quenching molecules do not affect the bacterial growth means that the risk of these molecules to induce resistance is minor. Due to the importance of QS in the bacterial pathogenesis process, it becomes an ideal target to control bacterial pathogens with an increased interest to identify QS inhibitors (quorum quenching) [ 12 , 13 , 14 ]. Several QS inhibitors have been identified, and among them, different phytochemicals [ 15 , 16 ], bacteriophages [ 17 , 18 ], and metal nanoparticles [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities

Faleiro

Marques

Martins

et al. 2022

Biology

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The increase in bacterial resistance to antibiotics has led researchers to find new compounds or find combinations between different compounds with potential antibacterial action and with the ability to prevent the development of antibiotic resistance. Polyoxotungstates (POTs) are inorganic clusters that may fulfill that need, either individually or in combination with antibiotics. Herein, we report the ability of the polyoxotungstates (POTs) with Wells-Dawson P2W18, P2W17, P2W15, and Preyssler P5W30 type structures to differently affect Gram-negative and Gram-positive microorganisms, either susceptible or resistant to antibiotics. The compound P5W30 showed the highest activity against the majority of the tested bacterial strains in comparison with the other tested POTs (P2W15, P2W17 and P2W18) that did not show inhibition zones for the Gram-negative bacteria, A. baumanii I73775, E. coli DSM 1077, E. coli I73194, K. pneumoniae I7092374, and P. aeruginosa C46281). Generally, the results evidenced that Gram-positive bacteria are more susceptible to the POTs tested. The compound P5W30 was the one most active against S. aureus ATCC 6538 and MRSA 16, reaching <0.83 mg·mL−1 (100 μM) and 4.96 mg·mL−1 (600 μM), respectively. Moreover, it was verified by NMR spectroscopy that the most promising POT, P5W30, remains intact under all the experimental conditions, after 24 h at 37 °C. This prompted us to further evaluate the anti-quorum sensing activity of P5W30 using the biosensor Chromobacterium violaceum CV026, as well as its antibiofilm activity both individually and in combination with the antibiotic cefoxitin against the methicillin-resistant Staphylococcus aureus 16 (MRSA16). P5W30 showed a synergistic antibacterial effect with the antibiotic cefoxitin and chloramphenicol against MRSA 16. Moreover, the antibiofilm activity of P5W30 was more pronounced when used individually, in comparison with the combination with the antibiotic cefoxitin. Finally, the antiviral activity of P5W30 was tested using the coliphage Qβ, showing a dose-dependent response. The maximum inactivation was observed at 750 μM (6.23 mg·mL−1). In sum, P5W30 shows anti-quorum sensing and antibiofilm activities besides being a potent antibacterial agent against S. aureus and to exhibit antiviral activities against enteric viruses.

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Facile access to novel furoyl-based amide retroisoster inhibitors of LuxR-regulated quorum sensing: conformation analysis and docking studies

Soulère,

Queneau

2024

Med Chem Res

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Chemical probe of AHL modulators on quorum sensing in Gram-Negative Bacteria and as antiproliferative agents: A review

Cited by 25 publications

References 63 publications

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities

Facile access to novel furoyl-based amide retroisoster inhibitors of LuxR-regulated quorum sensing: conformation analysis and docking studies

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