Chemical Profile and Antibacterial Activity of a Novel Spanish Propolis with New Polyphenols also Found in Olive Oil and High Amounts of Flavonoids

Fernández-Calderón, María Coronada; Navarro-Pérez, María Luisa; Blanco-Roca, María Teresa; Gómez-Navia, Carolina; Pérez-Giraldo, Ciro; Vadillo-Rodrı́guez, Virginia

doi:10.3390/molecules25153318

Cited by 25 publications

(21 citation statements)

References 63 publications

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“…In recent years several studies have shown the antibacterial and antifungal effects of propolis, with variations according to its different origins [16][17][18], but few studies have been performed on clinical isolates of C. glabrata [19][20][21]. In previously published works we have verified the excellent antibacterial activity of the novel Spanish Ethanolic Extract of Propolis (SEEP) [22] and its foreseeable initial mechanism of action against gram-negative and grampositive bacteria [23]. The chemical profile of SEEP was determined by Liquid chromatography-mass spectrometry (LC-MS) and high-resolution gas chromatography coupled with mass spectrometry (GC-MS), and revealed for the first time the presence of olive oil compounds (Vanillic acid, 1-Acetoxypinoresinol, p-HPEA-EA and 3,4-DHPEA-EDA).…”

Section: Introductionmentioning

confidence: 59%

“…In addition, it should be noted that it contains high amounts of polyphenols (205 ± 34 mg GAE/g), with unusually more than half of these from the flavonoid class (127 ± 19 mg QE/g). The TFC/TPC ratio (total flavonoids content/total polyphenols contents) (~0.6) was the highest ever reported, both in Spain and in many parts of the world [22].…”

Section: Introductionmentioning

confidence: 89%

“…The SEEP was filtered as received with a 0.20 μm syringe filter (Millipore, Merck, Darmstandt, Germany) and stored at 4°C until use. This stock of SEEP, 100% (v/v) is equivalent to 61.5 g/L dry weight of propolis [22],…”

Section: Propolis Samples Preparationmentioning

confidence: 99%

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Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Fernández-Calderón

Hernández-González

Gómez-Navia

et al. 2021

BMC Complement Med Ther

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Background Resistance to traditional antifungal agents is a considerable health problem nowadays, aggravated by infectious processes related to biofilm formation, usually on implantable devices. Therefore, it is necessary to identify new antimicrobial molecules, such as natural products, to develop new therapeutic strategies to prevent and eradicate these infections. One promising product is propolis, a natural resin produced by honeybees with substances from various botanical sources, beeswax and salivary enzymes. The aim of this work was to study the effect of a new Spanish ethanolic extract of propolis (SEEP) on growth, cell surface hydrophobicity, adherence and biofilm formation of Candida glabrata, a yeast capable of achieving high levels of resistance to available anti-fungal agents. Methods The antifungal activity of SEEP was evaluated in the planktonic cells of 12 clinical isolates of C. glabrata. The minimum inhibitory concentration (MIC) of propolis was determined by quantifying visible growth inhibition by serial plate dilutions. The minimum fungicide concentration (MFC) was evaluated as the lowest concentration of propolis that produced a 95% decrease in cfu/mL, and is presented as MFC50 and MFC90, which corresponds to the minimum concentrations at which 50 and 90% of the C. glabrata isolates were inhibited, respectively. Influence on cell surface hydrophobicity (CSH) was determined by the method of microbial adhesion to hydrocarbons (MATH). The propolis effect on adhesion and biofilm formation was determined in microtiter plates by measurement of optical density (OD) and metabolic activity (XTT-assay) in the presence of sub-MIC concentrations of SEEP. Results SEEP had antifungal capacity against C. glabrata isolates, with a MIC50 of 0.2% (v/v) and an MFC50 of 0.4%, even in azole-resistant strains. SEEP did not have a clear effect on surface hydrophobicity and adhesion, but an inhibitory effect on biofilm formation was observed at subinhibitory concentrations (0.1 and 0.05%) with a significant decrease in biofilm metabolism. Conclusions The novel Spanish ethanolic extract of propolis shows antifungal activity against C. glabrata, and decreases biofilm formation. These results suggest its possible use in the control of fungal infections associated with biofilms.

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Section: Introductionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 89%

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Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Fernández-Calderón

Hernández-González

Gómez-Navia

et al. 2021

BMC Complement Med Ther

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“…Having such a large distribution over the temperate zones of the globe, poplar-type propolis is also very different in chemical compounds, although volatiles from the class of terpenoids and polyphenolic substances (phenolic acids and flavonoids) are the major compounds. A recent study by [ 71 ], identified a new type of propolis rich in flavonoids which exhibit also a very powerful antibacterial activity.…”

Section: Poplar-type Propolismentioning

confidence: 99%

Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis

Dezmirean

Pașca

Moise

et al. 2020

Plants

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Propolis is a resinous mixture, made by the honeybees from substances collected from tree or other plant buds, plant exudates, or resins found in the stem, branches, or leaves of different plants. The geographical origin of propolis is given by plant sources from respective areas. Different studies have classified this bee product according to the vegetal material from the same areas. Poplar-type propolis has the widest spread in the world, in the temperate zones from Europe, Asia, or North America. The name is given by the main plant source from where the bees are collecting the resins, although other vegetal sources are present in the mentioned areas. Different Pinus spp., Prunus spp., Acacia spp. and also Betula pendula, Aesculus hippocastanum, and Salix alba are important sources of resins for “poplar-type” propolis. The aim of this review is to identify the vegetal material’s chemical composition and activities of plant resins and balms used by the bees to produce poplar-type propolis and to compare it with the final product from similar geographical regions. The relevance of this review is to find the similarities between the chemical composition and properties of plant sources and propolis. The latest determination methods of bioactive compounds from plants and propolis are also reviewed.

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“…More than 200 flavonoids have been identified from various kinds of propolis around the world [ 4 ]. Chinese propolis (CP), one of the Populus type of propolis, mainly contains flavonoids and phenolic compounds and has exhibited extensive pharmacological activities including antibacterial [ 5 ], anti-inflammatory [ 6 ], antivirus [ 7 ], antitumor [ 8 ], antioxidant [ 9 ], and immunoregulation activities [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chinese Poplar Propolis Inhibits MDA-MB-231 Cell Proliferation in an Inflammatory Microenvironment by Targeting Enzymes of the Glycolytic Pathway

Liu

Liu³

et al. 2021

Journal of Immunology Research

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Propolis is rich in flavonoids and has excellent antitumor activity. However, little is known about the potential effects of propolis on glycolysis in tumor cells. Here, the antitumor effects of propolis against human breast cancer MDA-MB-231 cells in an inflammatory microenvironment stimulated with lipopolysaccharide (LPS) were investigated by assessing the key enzymes of glycolysis. Propolis treatment obviously inhibited MDA-MB-231 cell proliferation, migration and invasion, clone forming, and angiogenesis. Proinflammatory mediators, including tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6, as well as NLRP3 inflammasomes, were decreased following propolis treatment when compared with the LPS group. Moreover, propolis treatment significantly downregulated the levels of key enzymes of glycolysis–hexokinase 2 (HK2), phosphofructokinase (PFK), pyruvate kinase muscle isozyme M2 (PKM2), and lactate dehydrogenase A (LDHA) in MDA-MB-231 cells stimulated with LPS. After treatment with 2-deoxy-D-glucose (2-DG), an inhibitor of glycolysis, the inhibitory effect of propolis on migration was not significant when compared with the LPS group. In addition, propolis increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential. Taken together, these results indicated that propolis targeted key enzymes of glycolysis to suppress the proliferation of MDA-MB-231 cells in an inflammatory microenvironment. These studies provide a molecular basis for propolis as a natural anticancer agent against breast cancer.

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Chemical Profile and Antibacterial Activity of a Novel Spanish Propolis with New Polyphenols also Found in Olive Oil and High Amounts of Flavonoids

Cited by 25 publications

References 63 publications

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis

Chinese Poplar Propolis Inhibits MDA-MB-231 Cell Proliferation in an Inflammatory Microenvironment by Targeting Enzymes of the Glycolytic Pathway

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