Chemical profile and in vitro antibacterial potential of essential oils and hydrolat extracts from aerial parts of three wild species of Moroccan Thymus

Moukhles, Abderrahman; Ellaghdach, Anas; Driss, Abdenbi Ben; Amrani, Mohamed Amin El; Aghmiz, Ali; Mansour, Ahmed Ibn

doi:10.1016/j.sciaf.2022.e01434

Cited by 7 publications

(7 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparison of the chemical composition of EOs and HSs of herbs showed that EOs contained more compounds, and several studies are in agreement with that [18,47,63]. The chemical profile of M. pulegium HS was the same with the respective EO, but in lower proportions, with the notable exception the absence of 3-octanol.…”

Section: Pulegium Essential Oil and Hydrosol Extract Chemical Profilesupporting

confidence: 71%

“…Of note, carvacrol was detected in O. vulgare HS, apparently in a much higher percentage compared to EO. This profound difference could be attributed to the fact that some non-polar molecules, especially terpene hydrocarbons (α-pinene, α-myrcene, o-cymene, α-and β-terpinene), are detected only in EOs [47].…”

Section: O Vulgare Essential Oil and Hydrosol Extract Chemical Profilementioning

confidence: 99%

See 1 more Smart Citation

Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens

Bairamis,

Sotiropoulou,

Tsadila

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

In this study, the chemical composition of essential oils (EOs) extracted from Origanum vulgare ssp. hirtum Lamiaceae, (oregano), Salvia officinalis Lamiaceae (sage), Mentha pulegium Lamiaceae (pennyroyal), and respective hydrosols (HSs) has been investigated by Gas Chromatography–Mass Spectrometry (GC-MS). The antimicrobial activity was assessed against two oral pathogens: Gram-positive bacterium Streptococcus mutans and the fungus Candida albicans by determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal concentration (MBC/MFC). Three-fold diluted solutions were dispensed into each well of a 96-well microtiter plate and, after incubation, MIC was determined by visual monitoring. The MBC/MFC was determined by transferring a small quantity of sample contained in each replicate well of the microtiter plates to appropriate culture media using a microplate replicator. The EOs of the tested herbs showed antimicrobial properties, especially the EO oil of O. vulgare, which exerted the highest antimicrobial activity. HSs of S. officinalis and M. pulegium exerted no antimicrobial activity, in contrast to oregano HS, which displayed strong antimicrobial activity. In all cases, a higher number of compounds were detected in EOs than in the corresponding HSs. The major compounds of sage EO were detected to be α-thujone (25.1%), 1,8-cineole (15.8%) and β-pinene (10.0%), while the HS was characterized by the presence of 1,8-cineole (32.6%), borneol (22.6%) and α-thujone (22.4%). Pennyroyal EO and HS consists mainly of pulegone (62.1 and 50.6%, respectively). Carvacrol was the major component present in EO (63%) and HS (97.3%) of oregano, probably contributing to the antimicrobial activity. Further research is needed in order to elucidate the antimicrobial mechanisms of specific compounds present in essential oils and hydrosols of Lamiaceae grown in Greece against oral pathogens.

show abstract

Section: Pulegium Essential Oil and Hydrosol Extract Chemical Profilesupporting

confidence: 71%

Section: O Vulgare Essential Oil and Hydrosol Extract Chemical Profilementioning

confidence: 99%

Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens

Bairamis,

Sotiropoulou,

Tsadila

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…The antioxidant capacity of Thymus species has been well researched. The most relevant chemotypes of Thymus species have been reported to be rich in phenolic monoterpenes, such as thymol and carvacrol [ 41 ]. In most such studies, phenolics, due to their chemical structures, which allow them to donate hydrogen to free radicals, were introduced as the main factor contributing to the antioxidant activity of the species [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical, Morphological, and Physiological Variation in Different Ajowan (Trachyspermum ammi L.) Populations as Affected by Salt Stress, Genotype × Year Interaction and Pollination System

Mirniyam

Rahimmalek

Arzani

et al. 2023

IJMS

View full text Add to dashboard Cite

In the present research, 28 populations of ajowan (Trachyspermum ammi L.) were evaluated for agro-morphological traits and essential oil yield in two consecutive years. Then, selected ajowan populations from these two years were used for further morphophysiological and biochemical studies under different salinity levels (control, 60, 90, and 120 mM NaCl). The main components of the oil were thymol (32.7–54.29%), γ-terpinene (21.71–32.81%), and p-cymene (18.74–26.16%). Salt stress caused an increase in essential oil content in the Esfahfo and Qazvin populations. The highest total phenolic and flavonoid contents were found in the Arak population grown in 60 mM NaCl (183.83 mg TAE g−1 DW) and the Yazd population grown in 90 mM NaCl (5.94 mg QE g−1 DW). Moreover, the Yazd population exhibited the strongest antioxidant activity based on DPPH (IC50 = 1566 µg/mL) under 60 mM NaCl and the highest reducing power (0.69 nm) under 120 mM NaCl. The results revealed that low and moderate salt stress improves the phytochemicals of ajowan seeds, which are useful for pharmaceutical and food applications. In this research, some morphological traits, as well as essential oil yield, were evaluated in open pollinated versus self-pollinated plants. As a result, plant height, number of flowering branches, and crown diameter significantly decreased in some populations, while a significant increase was obtained for number of flowers per umbel and seed numbers per umbel. Finally, self-pollination of ajowan might provide new insights for further breeding programs to increase oil or thymol content in ajowan.

show abstract

“…Based on the chemometric investigation, this species includes the seven main chemotypes, including thymol, and carvacrol which, according to literature data, are the most common, followed by linalool, α-terpinyl acetate, thymol/p-cymene/γ-terpinene, 1,8-cineole/myrcene/spathulenol, and 1,8-cineole/α-terpineol [19]. Traditionally, this plant has been used as medicine, but has also been used as a spice [20,22]. Recent studies of different chemotypes of T. zygis essential oils have demonstrated their beneficial antimicrobial, antibiofilm, anti-inflammatory, antiseptic, antioxidant, and antispasmodic effects [20,[23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Thymus zygis, Valuable Antimicrobial (In Vitro and In Situ) and Antibiofilm Agent with Potential Antiproliferative Effects

Vukić,

Čmiková,

Hsouna

et al. 2023

Plants

View full text Add to dashboard Cite

With the growing issues of food spoilage, microbial resistance, and high mortality caused by cancer, the aim of this study was to evaluate T. zygis essential oil (TZEO) as a potential solution for these challenges. Here, we first performed GC/MS analysis which showed that the tested TZEO belongs to the linalool chemotype since the abundance of linalool was found to be 38.0%. Antioxidant activity assays showed the superiority of TZEO in neutralizing the ABTS radical cation compared to the DPPH radical. The TZEO was able to neutralize 50% of ABTS•+ at the concentration of 53.03 ± 1.34 μg/mL. Antimicrobial assessment performed by employing disc diffusion and minimal inhibitory concentration assays revealed TZEO as a potent antimicrobial agent with the highest inhibition activity towards tested gram-negative strains. The most sensitive on the treatment with TZEO was Enterobacter aerogenes showing an MIC 50 value of 0.147 ± 0.006 mg/mL and a MIC 90 value of 0.158 ± 0.024 mg/mL. Additionally, an in situ analysis showed great effects of TZEO in inhibiting gram-negative E. coli, P. putida, and E. aerogenes growing on bananas and cucumbers. Treatment with the TZEO vapor phase in the concentration of 500 μg/mL was able to reduce the growth of these bacteria on the food models to the extent > 90%, except for E. coli growth on the cucumber, which was reduced to the extent of 83.87 ± 4.76%. Furthermore, a test on the antibiofilm activity of the tested essential oil revealed its biofilm prevention effects against Salmonella enterica which forms biofilms on plastic and stainless-steel surfaces. Performed tests on the TZEO effects towards cell viability showed no effects on the normal MRC-5 cell line. However, the results of MTT assay of TZEO effects on three cancer cell lines (MDA-MB-231, HCT-116, and K562) suggest that TZEO exerted the strongest effects on the inhibition of the viability of MDA-MB-231 cells, especially after long-term treatment in the highest concentration applied with reducing the viability of the cells to 57%. Additionally, results of NBT and Griess assays suggest that TZEO could be a convenient candidate for future testing for developing novel antitumor therapies.

show abstract

Chemical profile and in vitro antibacterial potential of essential oils and hydrolat extracts from aerial parts of three wild species of Moroccan Thymus

Cited by 7 publications

References 65 publications

Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens

Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens

Phytochemical, Morphological, and Physiological Variation in Different Ajowan (Trachyspermum ammi L.) Populations as Affected by Salt Stress, Genotype × Year Interaction and Pollination System

Thymus zygis, Valuable Antimicrobial (In Vitro and In Situ) and Antibiofilm Agent with Potential Antiproliferative Effects

Contact Info

Product

Resources

About