Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

Abstract

Cyperus species represent a group of cosmopolitan plants used in folk medicine to treat several diseases. In the current study, the phytochemical profile of Cyperus laevigatus ethanolic extract (CLEE) was assessed using UPLC-QTOF–MS/MS. The protective effect of CLEE at 50 and 100 mg /kg body weight (b.w.) was evaluated on hepatorenal injuries induced by thioacetamide (100 mg/kg) via investigation of the extract’s effects on oxidative stress, inflammatory markers and histopathological changes in the liver and kidney. UPLC-QTOF–MS/MS analysis of CLEE resulted in the identification of 94 compounds, including organic and phenolic acids, flavones, aurones, and fatty acids. CLEE improved the antioxidant status in the liver and kidney, as manifested by enhancement of reduced glutathione (GSH) and coenzyme Q10 (CoQ10), in addition to the reduction in malondialdehyde (MDA), nitric oxide (NO), and 8-hydroxy-2′-deoxyguanosine (8OHdG). Moreover, CLEE positively affected oxidative stress parameters in plasma and thwarted the depletion of hepatorenal ATP content by thioacetamide (TAA). Furthermore, treatment of rats with CLEE alleviated the significant increase in plasma liver enzymes, kidney function parameters, and inflammatory markers. The protective effect of CLEE was confirmed by a histopathological study of the liver and kidney. Our results proposed that CLEE may reduce TAA-hepatorenal toxicity via its antioxidant and anti-inflammatory properties suppressing oxidative stress.

Apigenin role against thioacetamide-triggered liver fibrosis: Deciphering the PPARγ/TGF-β1/NF-κB and the HIF/FAK/AKT pathways

“…In the current work, liver fibrosis was induced in rats using the TAA method. TAA, a potent hepatotoxic agent, is metabolized by Cytochrome 450 enzyme in the liver and is transformed into toxic compounds, TAAS-oxide and TAAS-dioxide, via oxidative chains (25). Liver necrosis, fibrosis, cirrhosis, and finally, HCC were induced upon TAA administration which is caused by free radical-mediated lipid peroxidation (25).…”

Prophylactic Resveratrol Ameliorates Thioacetamide Hepatotoxicity in A Dose-Dependent Fashion Through the Regulation of Gene Expression Levels of MiR-155 and MiR-21

“…The Kaplan-Meier cumulative hazard curve for the optimal dose of RSV that minimize the TAA hepatotoxic effect of DISCUSSION Chronic exposure to thioacetamide (TAA) induced intense inflammatory and oxidative stress as manifested by significantly higher serum levels of inflammatory cytokines and serum MDA levels with a concomitant decrease of serum anti-inflammatory cytokines and antioxidants in samples of all animals even those received prophylaxis. In line these results, multiple animal models documented the deleterious effects of TAA on the immune and redox milieus (Bashandy et al,2018;Yang et al,2019;Ahmed et al,2022;Ayoub et al,2022). Moreover, TAA-hepatotoxicity was associated with increased serum levels of AST, ALT, bilirubin, and decreased serum albumin levels, and these changes paralleled the disturbed inflammatory and redox milieus for TAA-induced hepatotoxicity.…”

In vivo hepatoprotective and nephroprotective effects ofStenocarpus sinuatusleaf extract against ifosfamide‐induced toxicity in rats

“…[10][11][12][13][14][15][16][17][18] Many plant families have been utilized in traditional medicine and have proved their biological effectiveness. [19][20][21][22][23][24] Several plants have been reported to prompt neuroprotective, hepatoprotective, and nephroprotective effectiveness. [25][26][27][28] The Proteaceae family represents one of the important medicinal plant families.…”