Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model

Khaledi, Mostafa; Makhmalzadeh, Behzad Sharif; Rezaie, Annahita; Nazemi, Melika; Safdarian, Mehdi; Nabavi, Mohammad Bagher

doi:10.1371/journal.pone.0241582

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Here in the survey, mice treated with S. deserti methanolic extract harboured lower levels of cytokines compared to the control group. Similarly, Ficus carica [29], and Dysidea avara [30] extracts showed high anti-psoriasis effects through the reduction in rates of inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 98%

Assessment of the anti-psoriasis effect of Scrophularia deserti methanolic extract in mice model

Khaleel,

Shareef,

Maryoosh

2024

SR: PS

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Psoriasis is an underestimated chronic and autoimmune skin disorder. Topical chemical agents are applied for psoriasis control and treatment, notwithstanding their subordinate efficiency or unsuccessful activities. As an alternative, herbal medicine can also be used in its treatment. The aim of the present study was performed to assess the anti-psoriasis effect of Scrophularia deserti in mice model. Materials and methods: S. deserti was purchased and used for methanolic extraction. Extract DPPH radical scavenging activity, polyphenol and flavonoid contents were examined. Sixty male mice were purchased, and psoriasis was induced using 10 days of topical administration of Imiquimod (62.5 mg). Mice were classified into 6 groups: non-psoriasis control (only received distilled water), psoriasis control (only received topical Imiquimod), two S. deserti treatments (topical 300 and 500 mg/kg), topical Betamethasone, and topical α-pinene 9 %. Cytokine distribution and histopathological properties were also determined. Results: the value at which the S. deserti methanolic extract scavenges 50 % of free radicals (IC50) was 602.71±15.33 µg/mL. The total S. deserti methanolic extract flavonoid and polyphenol contents were 16.85±1.12 mg QE/g and 58.47±3.25 mg GAE/g, respectively. IL-22, TNF-α, and IL-17A concentrations increased after psoriasis induction compared to the control group (P <0.05). Mice treated with Betamethasone harboured the lowest concentrations of IL-22, TNF-α, and IL-17A (P <0.05). Conclusions: Mice treated with S. deserti methanolic extract (500 mg/kg) also harboured significantly lower IL-22, TNF-α, and IL-17A (P <0.05) compared to α-pinene and S. deserti methanolic extract (300 mg/kg). Mice of the psoriasis control group showed significant epidermis hyperkeratosis, acanthosis, and crust with plentiful inflammatory cells. At the same time, mice treated with S. deserti methanolic extract (500 mg/kg) showed significant recovered tissue with normal skin epidermis and dermis, sebaceous glands, and follicles of the hair, besides the lowest rate of inflammatory reactions. Findings showed that the S. deserti methanolic extract (500 mg/kg) can efficiently be used as a practical substitute for psoriasis treatment. However, some supplementary research should be performed

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Section: Discussionmentioning

confidence: 98%

Assessment of the anti-psoriasis effect of Scrophularia deserti methanolic extract in mice model

Khaleel,

Shareef,

Maryoosh

2024

SR: PS

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“…Additionally, avarone, isolated from the Chinese marine sponge D. avara, inhibited the activity of four human cancer cell lines: Cervix (HeLa), lung (A549), breast (MDA231), and hepatoma (QGY7703) (45). Khaledi et al (25) showed that extracts from Persian Gulf D. avara possessed anti-inflammatory activity following seven days of treatment. Mice treated with the extract displayed a dose-dependent and significant improvement compared to controls.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have indicated that sponges within the Dysidea spp. possess secondary metabolites with diverse biological properties (21)(22)(23)(24)(25)(26). These studies collectively suggest that D. avara, along with its bioactive compounds, holds promise in the development of new anticancer strategies.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity and Molecular Docking Studies of the Fraction Containing Ergosta-14,22-dien-3-ol (3β, 5α, and 22E) from Dysidea avara Sponges

Nazemi,

Golshan,

Khaledi

et al. 2024

Jundishapur J Nat Pharm Prod

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Background: Cancer is a global health burden, and the discovery of novel therapeutic agents remains a critical pursuit. Marine organisms, including sponges, have emerged as a promising source of structurally diverse natural products with potential anticancer properties. Objectives: In the present study, the cytotoxic activity of the fraction containing Ergosta-14,22-dien-3-ol (3β, 5α, and 22E), a steroid compound derived from Dysidea avara, was evaluated against Jurkat/E6-1 and Hek293 cells. Methods: Multiple analytical techniques, including column chromatography, TLC, and GC-MS, were used to isolate and identify the compound from marine sponges from the Persian Gulf. The XTT assay determined the cytotoxic activity and western blot for P53 expression in the Jurkat/ E6-1 cell line. The compound was also docked within the poly (ADP-ribose) polymerase-1 (PARP1) and E3 ubiquitin-protein ligase (MDM2) to investigate its potential mechanism of action. Furthermore, the pharmacological properties of the compound were predicted using PerADME, SwissADME, and Molinspiration tools. Results: The results showed that Ergosta-14,22-dien-3-ol (3β, 5α, and 22E) exhibited significant cytotoxic activity against Jurkat/E6-1 cells with an IC50 of 26.59 μg/mL. Western blotting analysis demonstrated a noticeable increase in the expression of P53 protein in cells treated with 50 and 100 µg/mL of the compound. In silico analysis revealed adequate binding energy against PARP1 and E3 MDM2 receptors. The compound also demonstrated favorable pharmacokinetic characteristics, specifically in absorption, distribution, metabolism, and excretion (ADME). Conclusions: Ergosta-14,22-dien-3-ol (3β, 5α, and 22E) has the potential to be developed as a promising anti-cancer agent.

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Challenges in Psoriasis Research: A Systematic Review of Preclinical Models

Ubago-Rodríguez,

Quiñones-Vico,

Sánchez-Díaz

et al. 2024

Dermatology

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Introduction: Psoriasis is a chronic inflammatory skin disease with variable clinical presentation, multifactorial etiology and an immunogenetic basis. Several studies demonstrate that it results from a dysregulated interaction between skin keratinocytes, immune cells and the environment that leads to a persistent inflammatory process modulated by cytokines and T cells.The development of new treatment options requires increased understanding of pathogenesis. However, the successful implementation of effective drugs requires well-characterized and highly available preclinical models that allow researchers to quickly and reproducibly determine their safety and efficacy. Methods: A systematic search on PubMed and Scopus databases was performed and assessed to find appropriate articles about psoriasis models applying the key words previously defined. The PRISMA guidelines were employed. Results: A total of 45 original articles were selected that met the selection criteria. Among these, there are articles on in vivo, in vitro and ex vivo models, with the in vitro model being the majority due to its ease of use. Within animal models, the most widely used in recent years are chemically induced models using a compound known as imiquimod. However, the rest of the animal models used throughout the disease’s research were also discussed. On the other hand, in vitro models were divided into two- and three dimensions. The latter were the most used due to their similarity to human skin. Lastly, the ex vivo models were discussed, although they were the least used due to their difficulty in obtaining them. Conclusions: Therefore, this review summarizes the current preclinical models (in vivo, in vitro and ex vivo), discussing how to develop them, their advantages, limitations, and applications. There are many challenges to improve the development of the different models. However, research in these in vitro model studies could reduce the use of animals. This is favored with the use of future technologies such as 3D bioprinting or organ-on-a-chip technologies.

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Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model

Cited by 5 publications

References 33 publications

Assessment of the anti-psoriasis effect of Scrophularia deserti methanolic extract in mice model

Assessment of the anti-psoriasis effect of Scrophularia deserti methanolic extract in mice model

Cytotoxic Activity and Molecular Docking Studies of the Fraction Containing Ergosta-14,22-dien-3-ol (3β, 5α, and 22E) from Dysidea avara Sponges

Challenges in Psoriasis Research: A Systematic Review of Preclinical Models

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