Chemical profiling of DNA G-quadruplex-interacting proteins in live cells

Zhang, Xiaoyun; Spiegel, Jochen; Cuesta, Sergio Martínez; Adhikari, Santosh; Balasubramanian, Shankar

doi:10.1038/s41557-021-00736-9

Cited by 106 publications

(98 citation statements)

References 72 publications

(106 reference statements)

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“…End-resection is one of the first steps during HR that is mainly mediated by exonuclease 1 (EXO1) [105]. It has been shown in human cells that EXO1 is important for replication and resection near G4 structures [69]. Besides EXO1, DNA2 functions in the initiation of homology-mediated DSB repair by generating 3 ssDNA overhangs [106][107][108].…”

Section: Pathwaymentioning

confidence: 99%

The Relevance of G-Quadruplexes for DNA Repair

Linke

Limmer

Juranek

et al. 2021

IJMS

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DNA molecules can adopt a variety of alternative structures. Among these structures are G-quadruplex DNA structures (G4s), which support cellular function by affecting transcription, translation, and telomere maintenance. These structures can also induce genome instability by stalling replication, increasing DNA damage, and recombination events. G-quadruplex-driven genome instability is connected to tumorigenesis and other genetic disorders. In recent years, the connection between genome stability, DNA repair and G4 formation was further underlined by the identification of multiple DNA repair proteins and ligands which bind and stabilize said G4 structures to block specific DNA repair pathways. The relevance of G4s for different DNA repair pathways is complex and depends on the repair pathway itself. G4 structures can induce DNA damage and block efficient DNA repair, but they can also support the activity and function of certain repair pathways. In this review, we highlight the roles and consequences of G4 DNA structures for DNA repair initiation, processing, and the efficiency of various DNA repair pathways.

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Section: Pathwaymentioning

confidence: 99%

The Relevance of G-Quadruplexes for DNA Repair

Linke

Limmer

Juranek

et al. 2021

IJMS

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“…It can also resolve the 5′-UTR G4 RNA to regulate MAGE family member D2 (MAGED2) protein level ( McRae et al, 2020 ) and the 3′-UTR G4 RNA to suppress gene expression ( McRae et al, 2017 ). Most recently, DDX1, DDX24, DDX42, and DDX58 were identified to bind G4 DNA or RNA; however, the cellular functions are still elusive ( Zyner et al, 2019 ; Herviou et al, 2020 ; Zhang X. et al, 2021 ).…”

Section: G-quadruplex Unwinding Helicases and Their Functionsmentioning

confidence: 99%

The Cellular Functions and Molecular Mechanisms of G-Quadruplex Unwinding Helicases in Humans

Liu

Zhu

Wang

et al. 2021

Front. Mol. Biosci.

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G-quadruplexes (G4s) are stable non-canonical secondary structures formed by G-rich DNA or RNA sequences. They play various regulatory roles in many biological processes. It is commonly agreed that G4 unwinding helicases play key roles in G4 metabolism and function, and these processes are closely related to physiological and pathological processes. In recent years, more and more functional and mechanistic details of G4 helicases have been discovered; therefore, it is necessary to carefully sort out the current research efforts. Here, we provide a systematic summary of G4 unwinding helicases from the perspective of functions and molecular mechanisms. First, we provide a general introduction about helicases and G4s. Next, we comprehensively summarize G4 unfolding helicases in humans and their proposed cellular functions. Then, we review their study methods and molecular mechanisms. Finally, we share our perspective on further prospects. We believe this review will provide opportunities for researchers to reach the frontiers in the functions and molecular mechanisms of human G4 unwinding helicases.

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“…Taken together, these studies provide compelling evidence to further identify and investigate other DDX helicase genes and associated alterations that may drive cancer through perturbations of the genome. For example, DDX42 and DDX50 were found to be PARP1 interactors, DDX26B is a core subunit of the integrator transcriptional complex enriched at UV damage sites, and DDX24 was found to interact with G4 quadruplex DNA [89][90][91]. DNA G4-quadruplex structures may underpin DDX helicase transcription initiation mechanisms and/or stabilize R-loop structures [92,93].…”

Section: Discussionmentioning

confidence: 99%

DEAD-Box RNA Helicases and Genome Stability

Cargill

Venkataraman

Lee

2021

Genes

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DEAD-box RNA helicases are important regulators of RNA metabolism and have been implicated in the development of cancer. Interestingly, these helicases constitute a major recurring family of RNA-binding proteins important for protecting the genome. Current studies have provided insight into the connection between genomic stability and several DEAD-box RNA helicase family proteins including DDX1, DDX3X, DDX5, DDX19, DDX21, DDX39B, and DDX41. For each helicase, we have reviewed evidence supporting their role in protecting the genome and their suggested mechanisms. Such helicases regulate the expression of factors promoting genomic stability, prevent DNA damage, and can participate directly in the response and repair of DNA damage. Finally, we summarized the pathological and therapeutic relationship between DEAD-box RNA helicases and cancer with respect to their novel role in genome stability.

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Chemical profiling of DNA G-quadruplex-interacting proteins in live cells

Cited by 106 publications

References 72 publications

The Relevance of G-Quadruplexes for DNA Repair

The Relevance of G-Quadruplexes for DNA Repair

The Cellular Functions and Molecular Mechanisms of G-Quadruplex Unwinding Helicases in Humans

DEAD-Box RNA Helicases and Genome Stability

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