Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry

Li, Songlin; Song, Jing‐Zheng; Choi, Franky Fung-Kei; Qiao, Chun‐Feng; Zhou, Yan; Han, Quan‐Bin; Xu, Hong‐Xi

doi:10.1016/j.jpba.2008.11.007

Cited by 163 publications

(101 citation statements)

References 29 publications

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“…As shown in Table II, compound 20 produced ions of m/z 641, 497, and 341 in the negative ion mode and m/z 341, 301 in the positive ion mode which were the characteristic fragment ions of isomalto-paeoniflorin and its isomers. Based on the comparison with existed report [6], compound 20 was (Table II) and the literature data [6], the two peaks were identified as benzoylpaeoniflorin and isobenzoylpaeoniflorin, respectively. Accordingly, compound 41 was identified as benzoylpaeoniflorin sulfonate, and compound 26 was assigned as (orth) oxypaeoniflorin [6,14].…”

Section: Organic Acidsmentioning

confidence: 87%

“…They were all phenolic acid, because the common features of MS data observed were the loss of H, CH 3 , or HCOO groups, and UV spectrum as well as MS product ions were compared with those of standard compounds, they were definitively identified as caffeic acid, vanillic acid and ferulic acid, respectively. The details were summarized in Table I [6,13]. They were tentatively assigned as paeoniflorin sulfonate.…”

Section: Organic Acidsmentioning

confidence: 99%

“…Previous chemical studies of SWD were all based on the qualitation and quantitation of some major compounds, effective fractions, or the chemical composition of the four individual drugs [3][4][5][6][7][8][9]. To our knowledge, the comprehensive chemical analysis of the formula is still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Zhu

Duan

Tang

et al. 2014

Acta Chromatographica

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Summary. An efficient and robust method for analyzing constituents of a well-known traditional Chinese medicine (TCM) formula SiWu decoction (SWD) contains Angelicae Sinensis Radix (ASR), Chuanxiong Rhizoma (CR), Paeoniae Radix Alba (PRA), and Rehmanniae Radix Praeparata (RRP) by ultra high-performance liquid chromatography (UHPLC)/time-of-flight mass spectrometry (TOF-MS) was established. The method efficiently applied to the separation of 75 compounds, including organic acids, phthalides, phenylpropanoid glycosides, iridoid glycosides, monoterpene glycosides, and galloyl glycosides in the complex prescription, 52 compounds in this study can be unambiguously identified or tentatively characterized. The separation was achieved within 20 min at the optimized chromatographic conditions. Our study provided a reliable and high-efficient method for the understanding of the chemical basis of SWD.

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Section: Organic Acidsmentioning

confidence: 87%

Section: Organic Acidsmentioning

confidence: 99%

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Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Zhu

Duan

Tang

et al. 2014

Acta Chromatographica

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“…Two literature references provide the most comprehensive information on the chemical constituents present in P lactiflora roots [51,52] . Monoterpene glycosides originating from Chishao could be important for XueBiJing therapy.…”

Section: Chishao Monoterpene Glycosides Present In Xuebijing Injectionmentioning

confidence: 99%

Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats

Cheng

Lin

et al. 2016

Acta Pharmacol Sin

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Aim: Monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) are believed to be pharmacologically important for the antiseptic herbal injection XueBiJing. This study was designed to characterize the pharmacokinetics and disposition of monoterpene glycosides. Methods: Systemic exposure to Chishao monoterpene glycosides was assessed in human subjects receiving an intravenous infusion and multiple infusions of XueBiJing injection, followed by assessment of the pharmacokinetics of the major circulating compounds. Supportive rat studies were also performed. Membrane permeability and plasma-protein binding were assessed in vitro. Results: A total of 18 monoterpene glycosides were detected in XueBiJing injection (content levels, 0.001-2.47 mmol/L), and paeoniflorin accounted for 85.5% of the total dose of monoterpene glycosides detected. In human subjects, unchanged paeoniflorin exhibited considerable levels of systemic exposure with elimination half-lives of 1.2-1.3 h; no significant metabolite was detected. Oxypaeoniflorin and albiflorin exhibited low exposure levels, and the remaining minor monoterpene glycosides were negligible or undetected. Glomerular-filtration-based renal excretion was the major elimination pathway of paeoniflorin, which was poorly bound to plasma protein. In rats, the systemic exposure level of paeoniflorin increased proportionally as the dose was increased. Rat lung, heart, and liver exposure levels of paeoniflorin were lower than the plasma level, with the exception of the kidney level, which was 4.3-fold greater than the plasma level; brain penetration was limited by the poor membrane permeability. Conclusion: Due to its significant systemic exposure and appropriate pharmacokinetic profile, as well as previously reported antiseptic properties, paeoniflorin is a promising XueBiJing constituent of therapeutic importance.

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“…The main active components [5,6] of Radix Paeoniae Rubra include paeoniflorin, albiflorin, hydroxy paeoniflorin, benzoyl paeoniflorin, and other monoterpene glycosides, which are considered as total paeony glycoside (TPG) collectively. The most abundant and active components in RPR are identified as paeoniflorin (PF) and albiflorin (AF) [7,8], which are reported to have many biological properties including antipyretic, antiallergic, antioxidative, anti-inflammatory, and anxiolytic activities [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

Zhu

You³

et al. 2017

Acta Chromatographica

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Summary. High-performance liquid chromatography coupled with photodiode array detection and evaporative light scattering detection (HPLC-DAD-ELSD) was established to determine paeoniflorin and albiflorin simultaneously in Radix Paeoniae Rubra. The assay was performed on a Diamonsil C 18 (4.6 mm × 250 mm, 5 μm) column by a gradient elution program with acetonitrile and aqueous formic acid (0.05% v/v) as mobile phase at a flow rate of 1.0 mL min −1 . The detection wavelength of DAD was 230 nm, and the evaporator tube temperature of ELSD was set at 110 °C with the nebulizing gas flow rate of 3 L min −1 . The temperature of column was kept at 30 °C. The linear ranges of paeoniflorin and albiflorin were within 0.050-1.510 mg mL −1 and 1.007-5.035 mg mL −1 . The recoveries of paeoniflorin and albiflorin were 96.2-102.9% and 95.0-102.4%, respectively, while the relative standard deviation (RSD) of them was 0.2-2.5%. This method was quick, simple, accurate, and specific. It could be used for the quality control of Radix Paeoniae Rubra. The proposed approach was expected as a powerful tool for the quality control of Radix Paeoniae Rubra.

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Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry

Cited by 163 publications

References 29 publications

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats

Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

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