Water is the earth's most valuable compound that is fundamental for humans as well as all other living forms and seems to be inexhaustible. Unfortunately the outlook for the world's fresh water supply is not very hopeful. Moreover shortage of fresh water throughout the world can be directly attributed to human misuse in the form of pollution. Water is used for number of purposes like drinking, bathing and washing etc. so it must be free from toxic materials for healthy human and aquatic life. Among surface waters, springs' water is usually considered as safe for drinking. In the present study, springs' water of Margalla Hill Zone III Islamabad was assessed to clarify the concern about the quality and safety of this water used for drinking purposes in terms of physico-chemical (color, temperature, pH, odor, turbidity, hardness, TDS, EC, alkalinity, DO, Cl -, NO 2 -, NO 3 2-, SO 4 2-, heavy metals) and microbiological parameters (total Coliform, Pseudomonas aeruginosa, Enterococcus and Staphylococcus auerus). For this purpose, 15 water samples were collected from five different sites of Margalla Hills. Results showed that WHO drinking water standards were exceeded for EC, DO, cadmium, lead and microbiological parameters which may be detrimental for human health if water is consumed on daily basis. All other selected parameters did not exceed WHO drinking water standards and therefore, water from the sampling area does not pose any significant threat to consumer's health.