Chemical Regulation of Fluorescence Lifetime

Dai, Jianan; Zhang, Xin

doi:10.1021/cbmi.3c00091

Cited by 10 publications

(1 citation statement)

References 91 publications

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“…Depending on the location of the probe within tumor tissue, oxygen concentration can be assessed inside the blood vessels, in the interstitial space and/or inside the cells [18][19][20]. Due to much longer phosphorescence decay time (µs to ms) compared to fluorescence, the measurements of oxygen can be combined with fluorescence imaging, including NAD(P)H FLIM [21,22].…”

Section: Introductionmentioning

confidence: 99%

Effects of Photodynamic Therapy on Tumor Metabolism and Oxygenation Revealed by Fluorescence and Phosphorescence Lifetime Imaging

Shirmanova,

Lukina,

Sirotkina

et al. 2024

IJMS

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This work was aimed at the complex analysis of the metabolic and oxygen statuses of tumors in vivo after photodynamic therapy (PDT). Studies were conducted on mouse tumor model using two types of photosensitizers—chlorin e6-based drug Photoditazine predominantly targeted to the vasculature and genetically encoded photosensitizer KillerRed targeted to the chromatin. Metabolism of tumor cells was assessed by the fluorescence lifetime of the metabolic redox-cofactor NAD(P)H, using fluorescence lifetime imaging. Oxygen content was assessed using phosphorescence lifetime macro-imaging with an oxygen-sensitive probe. For visualization of the perfused microvasculature, an optical coherence tomography-based angiography was used. It was found that PDT induces different alterations in cellular metabolism, depending on the degree of oxygen depletion. Moderate decrease in oxygen in the case of KillerRed was accompanied by an increase in the fraction of free NAD(P)H, an indicator of glycolytic switch, early after the treatment. Severe hypoxia after PDT with Photoditazine resulted from a vascular shutdown yielded in a persistent increase in protein-bound (mitochondrial) fraction of NAD(P)H. These findings improve our understanding of physiological mechanisms of PDT in cellular and vascular modes and can be useful to develop new approaches to monitoring its efficacy.

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Section: Introductionmentioning

confidence: 99%

Effects of Photodynamic Therapy on Tumor Metabolism and Oxygenation Revealed by Fluorescence and Phosphorescence Lifetime Imaging

Shirmanova,

Lukina,

Sirotkina

et al. 2024

IJMS

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The Synthesis of Functionalized Carbonized Polymer Dots via Reversible Assembly of Oligomers for Anti‐Counterfeiting, Catalysis, and Gas storage

Wang,

Qin,

Wang

et al. 2024

Advanced Science

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Carbonized polymer dots (CPDs) have shown exceptional potential across a wide range of applications. However, their practical utilization is significantly greatly impeded by the lack of precise control over their structures and functionalities. Consequently, the development of controlled synthesis strategies for CPDs with well‐defined structures and tailored functionalities remains a critical challenge in the field. Here, the controlled synthesis of functional CPDs with reversible assembly properties via airflow‐assisted melt polymerization, followed by a one‐step post‐synthetic doping strategy, is reported. This synthetic approach achieves high product yield, uniform and tunable structures, as well as customized functionalities including solid‐state emission, enhanced catalytic performance (3.5–45 times higher than conventional methods), and selective gas storage in the resulting CPDs. The ability to tailor the properties of CPDs through controlled synthesis opens up new opportunities for their practical application in photocatalysis and gas storage.

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The Role of Spacers as a Probe in Variation of Photoluminescence Properties of Mono‐ and Bi‐Nuclear Boron Compounds

Kumar Yadav,

Parveen,

Mondal

et al. 2024

Chemistry An Asian Journal

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A series of N,O donor‐based mono‐ and binuclear four‐coordinated boron complexes were synthesized. Depending on the substitution and spacer, these complexes exhibit intense blue, green and yellow emission in solution states. Notably, the fluorescence quantum yields (ФF) and fluorescence decay (lifetime, τ) of mononuclear boron complexes (2a‐2e) were higher than the binuclear boron complexes (2f‐2k). The lowest lifetime and quantum yield in binuclear boron complexes were due to intramolecular rotation induced non radiative processes. The disulphide spacer‐based boron complexes 2i‐2k showed aggregation‐caused quenching in the THF/H2O mixture whereas no other complexes were ACQ responsive. These complexes show large Stokes shift, one of them i.e. 2ehas the highest Stokes shift of 130 nm among them. Further, the electrochemical study suggests the presence of two redox incidences. Theoretical studies show close corroboration between the TD‐DFT computed and experimentally measured absorption maxima as well as DFT (GIAO) calculated and experimentally measured 11B NMR values. This complements the appropriate selection of the theoretical methods to shed light on the electronic transitions in the mono‐ and binuclear BF2 complexes.

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Chemical Regulation of Fluorescence Lifetime

Cited by 10 publications

References 91 publications

Effects of Photodynamic Therapy on Tumor Metabolism and Oxygenation Revealed by Fluorescence and Phosphorescence Lifetime Imaging

Effects of Photodynamic Therapy on Tumor Metabolism and Oxygenation Revealed by Fluorescence and Phosphorescence Lifetime Imaging

The Synthesis of Functionalized Carbonized Polymer Dots via Reversible Assembly of Oligomers for Anti‐Counterfeiting, Catalysis, and Gas storage

The Role of Spacers as a Probe in Variation of Photoluminescence Properties of Mono‐ and Bi‐Nuclear Boron Compounds

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