Chemical screening for promotion of adventitious root formation in Eucalyptus globulus

Negishi, Naoki; Oishi, Masatoshi; Kawaoka, Akiyoshi

doi:10.1186/1753-6561-5-s7-p139

Cited by 13 publications

(9 citation statements)

References 2 publications

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“…In poplar cuttings, anti-auxins present at one of these phases caused significant inhibition of AR (Bellamine et al, 1998). Moreover, Negishi et al (2011) compared easy and difficult-to-root lines of E. globulus and verified that IAA level was twofold higher in the easy rooting line, confirming the importance of IAA in AR.…”

Section: Phytohormonal Balances: the Seesaw Of Promotion Versus Reprementioning

confidence: 68%

“…A screen for chemicals that cause inhibition of cytochrome P-450 identified one chemical, MA65, which led to an increase in the number of roots of Arabidopsis seedlings and twofold higher IAA levels compared to the untreated Arabidopsis (Negishi et al, 2011). The observed phenotype was similar to the mutant superrot2 ( sur2 ) which contains high concentrations of free IAA (Delarue et al, 1998) due to a defect in the SUR2 gene, which encodes the CYP83B1 protein, a cytochrome P450-dependent monooxygenase (Barlier et al, 2000).…”

Section: Phytohormonal Balances: the Seesaw Of Promotion Versus Reprementioning

confidence: 99%

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When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Costa¹,

Almeida²,

Ruedell³

et al. 2013

Front. Plant Sci.

314

280

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Adventitious rooting (AR) is a multifactorial response leading to new roots at the base of stem cuttings, and the establishment of a complete and autonomous plant. AR has two main phases: (a) induction, with a requirement for higher auxin concentration; (b) formation, inhibited by high auxin and in which anatomical changes take place. The first stages of this process in severed organs necessarily include wounding and water stress responses which may trigger hormonal changes that contribute to reprogram target cells that are competent to respond to rooting stimuli. At severance, the roles of jasmonate and abscisic acid are critical for wound response and perhaps sink strength establishment, although their negative roles on the cell cycle may inhibit root induction. Strigolactones may also inhibit AR. A reduced concentration of cytokinins in cuttings results from the separation of the root system, whose tips are a relevant source of these root induction inhibitors. The combined increased accumulation of basipetally transported auxins from the shoot apex at the cutting base is often sufficient for AR in easy-to-root species. The role of peroxidases and phenolic compounds in auxin catabolism may be critical at these early stages right after wounding. The events leading to AR strongly depend on mother plant nutritional status, both in terms of minerals and carbohydrates, as well as on sink establishment at cutting bases. Auxins play a central role in AR. Auxin transporters control auxin canalization to target cells. There, auxins act primarily through selective proteolysis and cell wall loosening, via their receptor proteins TIR1 (transport inhibitor response 1) and ABP1 (Auxin-Binding Protein 1). A complex microRNA circuitry is involved in the control of auxin response factors essential for gene expression in AR. After root establishment, new hormonal controls take place, with auxins being required at lower concentrations for root meristem maintenance and cytokinins needed for root tissue differentiation.

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Section: Phytohormonal Balances: the Seesaw Of Promotion Versus Reprementioning

confidence: 68%

Section: Phytohormonal Balances: the Seesaw Of Promotion Versus Reprementioning

confidence: 99%

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Costa¹,

Almeida²,

Ruedell³

et al. 2013

Front. Plant Sci.

314

280

View full text Add to dashboard Cite

show abstract

“…In P. taeda stem cuttings, the inability to root in mature cuttings was due to the lack of cell capacity to organize root meristems in the presence of auxin (Greenwood et al ). More recently, Negishi et al () compared E. globulus easy‐ and difficult‐to‐root lines, and showed that the indole‐3‐acetic acid (IAA) level of the easy‐rooting line was twice that of the difficult‐rooting line, confirming the importance of auxin in AR formation ability.…”

Section: Ar Formation Integrates Multiple Hormonal Pathwaysmentioning

confidence: 99%

Adventitious root formation in tree species: involvement of transcription factors

Legué

Rigal

Bhalerao

2014

Physiologia Plantarum

121

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Adventitious rooting is an essential step in the vegetative propagation of economically important horticultural and woody species. Populus has emerged as an experimental model for studying processes that are important in tree growth and development. It is highly useful for molecular genetic analysis of adventitious roots in trees. In this short review, we will highlight the recent progress made in the identification of transcription factors involved in the control of adventitious rooting in woody species. Their regulation will be discussed.

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“…On the other hand, anti‐auxin agents applied at AR early phases causes significant inhibition of AR in poplar cuttings (Bellamine et al ., ). In addition, the IAA content of an easily rooted genotype was higher than of a difficult to root genotype of Eucalyptus globulus (De Almeida et al ., ; Negishi et al ., ). These observations have demonstrated the important role of auxin in AR induction.…”

Section: Introductionmentioning

confidence: 97%

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

Shu

Zhou

Jiang

et al. 2018

Plant Biotechnology Journal

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Adventitious roots occur naturally in many species and can also be induced from explants of some tree species including Populus, providing an important means of clonal propagation. Auxin has been identified as playing a crucial role in adventitious root formation, but the associated molecular regulatory mechanisms need to be elucidated. In this study, we examined the role of PagFBL1, the hybrid poplar (Populus alba × P. glandulosa clone 84K) homolog of Arabidopsis auxin receptor TIR1, in adventitious root formation in poplar. Similar to the distribution pattern of auxin during initiation of adventitious roots, PagFBL1 expression was concentrated in the cambium and secondary phloem in stems during adventitious root induction and initiation phases, but decreased in emerging adventitious root primordia. Overexpressing PagFBL1 stimulated adventitious root formation and increased root biomass, while knock-down of PagFBL1 transcript levels delayed adventitious root formation and decreased root biomass. Transcriptome analyses of PagFBL1 overexpressing lines indicated that an extensive remodelling of gene expression was stimulated by auxin signalling pathway during early adventitious root formation. In addition, PagIAA28 was identified as downstream targets of PagFBL1. We propose that the PagFBL1-PagIAA28 module promotes adventitious rooting and could be targeted to improve Populus propagation by cuttings.

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Chemical screening for promotion of adventitious root formation in Eucalyptus globulus

Cited by 13 publications

References 2 publications

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Adventitious root formation in tree species: involvement of transcription factors

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

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