Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery

Tan, Justin L.; Zon, Leonard I.

doi:10.1016/b978-0-12-381320-6.00021-7

Cited by 73 publications

(26 citation statements)

References 57 publications

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“…Because of their small size, zebrafish embryos and larvae are amenable to high throughput assays that can be used to screen large numbers of chemical compounds for medically relevant effects on a broad range of physiological processes (145, 179). To date, compounds affecting hematopoietic stem cells, pancreatic beta cells, cancer cells (melanoma), liver injury, bone morphogenetic proteins (BMP) signaling, behavior and intestinal lipid absorption have been identified (134, 135, 154,157,188,194).…”

Section: Introductionmentioning

confidence: 99%

Zebrafish Models of Human Liver Development and Disease

Wilkins

Pack

2013

Comprehensive Physiology

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The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their disruption underlies a diverse group of heritable and acquired diseases that affect both pediatric and adult patients. Although experimental analyses used to study liver development and disease are typically performed in cell culture models or rodents, the zebrafish is increasingly used to complement discoveries made in these systems. Forward and reverse genetic analyses over the past two decades have shown that the molecular program for liver development is largely conserved between zebrafish and mammals, and that the zebrafish can be used to model heritable human liver disorders. Recent work has demonstrated that zebrafish can also be used to study the mechanistic basis of acquired liver diseases. Here, we provide a comprehensive summary of how the zebrafish has contributed to our understanding of human liver development and disease.

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Section: Introductionmentioning

confidence: 99%

Zebrafish Models of Human Liver Development and Disease

Wilkins

Pack

2013

Comprehensive Physiology

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“…The optical transparency of fish larvae permits the visualization of osteoblasts and osteoclasts in vivo, and although the lack of classical osteocytes in some teleosts is often used as an argument that fish are not a relevant model, this notion does not pertain to zebrafish. Furthermore, zebrafish have been widely used for chemical screens, 33 and particularly for osteoclast research this appears an attractive opportunity: osteoclasts are readily visible in fish larvae 34 (Figure 1d), and do respond to pathologically mineralized tissue (Apschner et al, in preparation). Hence, if compounds need to be tested for their effect on osteoclasts, this could be accomplished much quicker and more economically in fish than in mice.…”

Section: Resultsmentioning

confidence: 97%

A bone to pick with zebrafish

Mackay¹,

Apschner²,

Schulte‐Merker³

2013

BoneKEy Reports

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The development of high-throughput sequencing and genome-wide association studies allows us to deduce the genetic factors underlying diseases much more rapidly than possible through classical genetics, but a true understanding of the molecular mechanisms of these diseases still relies on integrated approaches including in vitro and in vivo model systems. One such model that is particularly suitable for studying bone diseases is the zebrafish (Danio rerio), a small fresh-water teleost that is highly amenable to genetic manipulation and in vivo imaging. Zebrafish physiology and genome organization are in many aspects similar to those of humans, and the skeleton and mineralizing tissues are no exception. In this review, we highlight some of the contributions that have been made through the study of mutant zebrafish that feature bone and/or mineralization disorders homologous to human diseases, including osteogenesis imperfecta, fibrodysplasia ossificans progressiva and generalized arterial calcification of infancy. The genomic and phenotypic similarities between the zebrafish and human cases are illustrated. We show that, despite some systemic physiological differences between mammals and teleosts, and a relative lack of a history as a model for bone research, the zebrafish represents a useful complement to mouse and tissue culture systems in the investigation of genetic bone disorders.

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“…In the case of zebrafish, screening conditions are already well established for embryos and larvae [69].…”

Section: Whole-animal Screensmentioning

confidence: 99%

Modeling colorectal cancer as a 3-dimensional disease in a dish: the case for drug screening using organoids, zebrafish, and fruit flies

Markstein

2013

Drug Discovery Today: Technologies

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This review discusses recent shifts in the understanding of colorectal cancer as a stem cell based disease, based on findings that tie patient prognosis to the presence of cancer stem cells in colorectal tumors. Currently no drugs specifically target CSCs in colorectal tumors.However, recent advances in the culturing of colorectal stem cells using mammalian organoids, zebrafish, and Drosophila offer promising avenues for anti-CSC drug discovery.

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Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery

Cited by 73 publications

References 57 publications

Zebrafish Models of Human Liver Development and Disease

Zebrafish Models of Human Liver Development and Disease

A bone to pick with zebrafish

Modeling colorectal cancer as a 3-dimensional disease in a dish: the case for drug screening using organoids, zebrafish, and fruit flies

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