Several samples of the common ferritic tool steel AISI 1045 were fatigued in cyclic load tests. The local distribution of the Von-Mieses stress VM was simulated using the finite elements method (FEM). In the regions of interest, where VM reaches maximum values, the defect distribution was measured spatially resolved by Doppler-spectroscopy (DBAR) employing the Bonn Positron Microprobe (BPM). The lateral distribution of the S-parameter, which could be described by a simple model derived from linear fracture mechanics, corresponds well with the simulated Von-Mieses stress