Chemical shift mapping of shikimate‐3‐phosphate binding to the isolated N‐terminal domain of 5‐enolpyruvylshikimate‐3‐phosphate synthase

Stauffer, Melissa E.; Young, John K.; Helms, Gregory L.

doi:10.1016/s0014-5793(01)02555-8

Cited by 18 publications

(16 citation statements)

References 15 publications

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“…Therefore, although both P318R and M425T are not involved in the PEP/glyphosate binding, considering that they are within the "emerging active site" of inner cleft, any mutation in this active site may indirectly induce structural adjustments in the hydrogen bonding networks. These adjustments result in positional shifts of the adjacent and nearby residues around S3P/PEP (36). Thus, these changes in P318R and M425T improved glyphosate tolerance.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Ochrobactrum 5-Enopyruvylshikimate-3-Phosphate Synthase

Tian

Xiong

et al. 2011

Appl Environ Microbiol

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A mutant of 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified after four rounds of DNA shuffling and screening. Its ability to restore the growth of the mutant ER2799 cell on an M9 minimal medium containing 300 mM glyphosate led to its identification. The mutant had mutations in seven amino acids: E145G, N163H, N267S, P318R, M377V, M425T, and P438L. Among these mutations, N267S, P318R, and M425T have never been previously reported as important residues for glyphosate resistance. However, in the present study they were found by site-directed mutagenesis to collectively contribute to the improvement of glyphosate tolerance. Kinetic analyses of these three mutants demonstrated that the effectiveness of these three individual amino acid alterations on glyphosate tolerance was in the order P318R > M425T > N267S. The results of the kinetic analyses combined with a three-dimensional structure modeling of the location of P318R and M425T demonstrate that the lower hemisphere's upper surface is possibly another important region for glyphosate resistance. Furthermore, the transgenic Arabidopsis was obtained to confirm the potential of the mutant in developing glyphosate-resistant crops.

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Section: Discussionmentioning

confidence: 99%

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Ochrobactrum 5-Enopyruvylshikimate-3-Phosphate Synthase

Tian

Xiong

et al. 2011

Appl Environ Microbiol

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“…The gene that produces the EPSPs enzyme is encoded and transcribed in the nucleus (Stauffer et al, 2001). The mRNA is transported to the cytoplasm, where the EPSPs protein is synthesized.…”

Section: Glyphosate Mode Of Actionmentioning

confidence: 99%

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

Roso

Vidal

2010

Planta daninha

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-Glyphosate is an herbicide that inhibits the enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPs) (EC 2.5.1.19). EPSPs is the sixth enzyme of the shikimate pathway, by which plants synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan and many compounds used in secondary metabolism pathways. About fifteen years ago it was hypothesized that it was unlikely weeds would evolve resistance to this herbicide because of the limited degree of glyphosate metabolism observed in plants, the low resistance level attained to EPSPs gene overexpression, and because of the lower fitness in plants with an altered EPSPs enzyme. However, today 20 weed species have been described with glyphosate resistant biotypes that are found in all five continents of the world and exploit several different resistant mechanisms. The survival and adaptation of these glyphosate resistant weeds are related toresistance mechanisms that occur in plants selected through the intense selection pressure from repeated and exclusive use of glyphosate as the only control measure. In this paper the physiological, biochemical, and genetic basis of glyphosate resistance mechanisms in weed species are reviewed and a novel and innovative theory that integrates all the mechanisms of non-target site glyphosate resistance in plants is presented.Keywords: membrane carrier proteins, herbicide, weed resistance.RESUMO -Glyphosate é uma glicina fosfonada e inibe a enzima 5-enolpiruvil-shikimato-3-fosfato sintase (EPSPS) (EC 2.5.1.19). EPSPS é a sexta enzima da rota do shikimato, na qual são sintetizados os compostos do metabolismo secundário e os aminoácidos aromáticos fenilalanina, tirosina e triptofano. Alguns autores hipotetizaram que seria improvável a evolução de plantas daninhas resistentes a este herbicida. As justificativas estariam relacionadas à limitada metabolização de glyphosate nas plantas, ao baixo nível de resistência obtido com a superexpressão do gene EPSPS e à alta penalidade de adaptação oriunda de mutações no gene EPSPS. Mas, atualmente estão descritas 18 espécies com biótipos resistentes distribuídas em 17 países. A sobrevivência e adaptação estão relacionadas aos mecanismos de resistência que ocorrem nas plantas daninhas selecionadas em função da intensa pressão de seleção do herbicida. Esta revisão objetiva apresentar e discutir evidências fisiológicas, bioquímicas e genéticas que fundamentam os principais mecanismos envolvidos na resistência ao herbicida glyphosate em plantas daninhas.Palavras-chave: herbicida, proteínas carregadoras de membrana, planta daninha.

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“…Trata-se de um herbicida sistêmico, não seletivo e de amplo espectro, com translocação via simplasto e com absorção facilitada por proteínas transportadoras de grupos fosfato, que estão presentes na membrana (Velini et al, 2009). A enzima EPSPs é codificada no núcleo e desempenha sua função no cloroplasto (Stauffer et al, 2001), catalisando a ligação dos compostos chiquimato-3-fosfato e fosfoenolpiruvato produzindo o enolpiruvilchiquimato-3-fosfato e fosfato inorgânico (Peterson et al, 1996). Segundo Hess (1993) o glyphosate é um inibidor não competitivo e competitivo, respectivamente, com os dois substratos.…”

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Baixas doses de glyphosate e seus efeitos no crescimento de

Meschede¹,

Velini²,

Carbonari³

2008

RBHerbicidas

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Resumo -Com o objetivo de verificar o efeito de baixas doses, simulando a deriva do herbicida glyphosate no crescimento e desenvolvimento inicial da Commmelina benghalensis foi realizado um trabalho em condições de casa-de-vegetação. A trapoeraba foi semeada superficialmente (aproximadamente 2 cm) em tubetes preenchidos com substrato. Após a germinação, deixou-se apenas uma planta por tubete e realizou-se aplicação dos tratamentos. Os tratamentos foram constituídos por diferentes doses de glyphosate, 2, 4, 8, 16, 20 e 24 g e.a. ha -1 e uma testemunha sem aplicação. Pelos resultados obtidos pode-se verificar que em geral, doses a partir de 2 g e.a. ha -1 podem causar efeitos no crescimento e desenvolvimento da parte aérea e radicular de plantas de C. bengalensis. Palavras-chave: Trapoeraba, deriva, EPSPs.Abstract -With the objective of verifying the effect of low doses, simulating glyphosate herbicide drift in growth and initial development of Commmelina benghalensis, a study was conducted in greenhouse conditions. C. benghalensis was superficially sowed (approximately 2 cm) in small tubes filled out with substratum. After the germination, just one plant by tube was kept and treatments were applied. Treatments were constituted by different glyphosate doses, 2, 4, 8, 16, 20 and 24 g a.e. ha -1 and a check without application. By obtained results, it was possible to verify that, doses starting from 2 g a.e. ha -1 may cause effects in growth and development of dossel and roots of C. benghalensis plants.

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Chemical shift mapping of shikimate‐3‐phosphate binding to the isolated N‐terminal domain of 5‐enolpyruvylshikimate‐3‐phosphate synthase

Cited by 18 publications

References 15 publications

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Ochrobactrum 5-Enopyruvylshikimate-3-Phosphate Synthase

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Ochrobactrum 5-Enopyruvylshikimate-3-Phosphate Synthase

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

Baixas doses de glyphosate e seus efeitos no crescimento de

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