Chemical Studies and Antibacterial Activity of the Root of Rumex abyssinicus

Abstract

One of the Ethiopian endemic plants used traditionally as medicine for different diseases is Rumex abyssinicus. It is widely known as ‘Meqmeqo’ in Amharic. The aim of this study was to isolate and characterize compounds from the root of R. abyssinicus and to test their bioactivities of solvent fractions of the extract. The crude extract was extracted from dried powdered root of the plant with methanol. Then it was fractionated with n‑hexane and ethyl acetate with increasing polarity. The yellow powder obtained from ethyl acetate fractionation was applied to column chromatographic separation that led to the isolation of two compounds, diisobutyl phthalate and emodine. Finally, antibacterial evaluation of the ethyl acetate and methanol soluble portions were carried out against four pathogenic bacteria. Both methanol and ethyl acetate soluble portions inhibited all selected bacterial in better at high concentrations.

Evaluation of the Antidiabetic Activity of Hydromethanolic Roots Extracts of Rumex abyssinicus Jacq: (Polygonaceae) in Swiss Albino Mice

“…e phytochemical screening test on the root extract revealed the presence of saponins, flavonoids, tannins, steroids, anthraquinones, alkaloids, quinones, phenol, and glycosides, but the absence of terpenoids [26]. e folklore antidiabetic uses, in vitro, and in vivo studies provide significant clues to conducting this experiment.…”

“…The ethanolic root extract of R.nepalensis demonstrated in vitro α -amylase inhibition, in vivo hypoglycemic activity in normal and glucose-loaded rats [ 25 ]. The phytochemical screening test on the root extract revealed the presence of saponins, flavonoids, tannins, steroids, anthraquinones, alkaloids, quinones, phenol, and glycosides, but the absence of terpenoids [ 26 ]. The folklore antidiabetic uses, in vitro, and in vivo studies provide significant clues to conducting this experiment.…”

Medicinal uses, chemical constituents and biological activities of Rumex abyssinicus: A Comprehensive review

“…Flavonoids, phenols, anthraquinones, saponins, cardiac glycosides, tannins, steroids, and quinones were detected in the qualitative phytochemical screening of R. abyssinicus, which could be linked to the plant's therapeutic effectiveness (Awoke & Gedamu, 2020;Kebede et al, 2021;Workineh, 2021;Workneh, 2017). Previous investigation into the chemical constituents of R. abyssinicus have led to the isolation of antraquinones (1-10), flavanols (11-13), terepens (14-15), fatty acid ( 16) and organic acid (17) (Figure 1).…”

In vitro antifungal, anti-inflammatory and cytotoxic activities of Rumex abyssinicus rhizome extract and bioassay-guided isolation of cytotoxic compounds from Rumex abyssinicus

“…The phytochemical studies of R. abyssinicus extract revealed the presence of tannins, flavonoids, phenols, quinones, alkaloids, cartenoids, phlobatannins, terpenoids, naphthalenes, stilbenoids, steroid, glycosides, saponins, fats and oils [7][8][9]. Many compounds are reported from R. abyssinicus including chrysophanol (1), physcion (2), emodin (3), citreorosein (4), β-sitosterol (5), aloe-emodin, emodic acid, chrysophanol-8-β-D-glucoside, emodin-β-8-O-D-glucoside, physcion-β-8-O-D-glucoside, bianthrone, emodin-chrysophanol, helminthosporin, epicatechin, epicatechin-3-O-gallate, epicatechin-3-O-(4″-O-methyl)gallate, methyl gallate, oxalic acid, phenolic acid, rumexamide, oleanolic acid, lupeol, 3β,28-dihydroxylup-20 (22)-ene 3βdihydroxylup-20(29)-en-28-oic acid, stigmasterol, stigmasterol-3-O-β-D-glucoside stigmastane-3,6-dione and ergosta-6,22-diene-3,5,8-triol [2,[10][11][12][13].…”