Chemical studies on crude drug processing. VI. Chemical structures of malonyl-ginsenosides Rb1, Rb2, Rc, and Rd isolated from the root of Panax ginseng C.A. Meyer.

Development of an Assay System for Saikosaponin a Using Anti-saikosaponin a Monoclonal Antibodies

“…From this evidence, such acyl saikosaponins may affect the correlation between both assay systems, since it is easily suggested that acyl SSa's have a similar cross-reactivity with SSa for the already discussed reason that the sugar moiety in saikosaponins is not related to its cross-reactivity. Therefore, the crude extract was treated with a mild alkaline solution, as previously reported, to give SSa without any change in aglycon skelton, similarly to saikogenin F. 22) Figure 3B shows the correlation of SSa concentration in Bupleuri radix between both assay systems, improving the correlation ratio to be rϭ0.999. From this result, the crude extract of Bupleuri radix should be treated with a mild alkaline solution before the ELISA assay, since it becomes evident that the reason for scattering of the correlation between HPLC and ELISA are the effect of acyl SSa.…”

“…For the elimination of an acyl group from acylated saikosaponins, the extracts were treated with 5% of KOH in MeOH at room temperature for 2 h, as previously reported, 22) then neutralized with 1 M HCl in MeOH, and assayed by ELISA.…”

Quality Control by Immunoaffinity Concentration Using Monoclonal Antibody

“…Ginsenosides are believed to account for the pharmacological activities of ginseng. It is well known that the concentrations of ginsenosides vary in the ginseng root or the root extracts depending on the method of extraction, subsequent treatment, or even the season of its collection (Kitagawa et al, 1989;Tanaka et al, 1989). Recently, the individual ginsenosides in ginseng are purified by preparative HPLC.…”

Pharmacologically Active Saikosaponin inBupleurum falcatumDetected by Competitive ELISA and Eastern Blotting Using Monoclonal Antibodies

“…Dried samples (20 mg) of various B. falcatum roots were powdered, extracted with methanol (MeOH) (0.5 mL) under sonication for 5 times, filtered, and then evaporated. For the elimination of acyl group from acylated saikosaponins, the extracts were treated with 5% of KOH in MeOH at room temperature for 2 hr as previously reported (Kitagawa et al, 1989), then neutralized with 1 M HCl in MeOH, and assayed by ELISA. For the immunohistochemical analysis of fresh leaves, leaves on the third node from the youngest leaf bud were used.…”