Chemical Studies on the Lipid Component of Endotoxin, With Special Emphasis on Its Relation to Biological Activities

Kasai, Nobuhiko

doi:10.1111/j.1749-6632.1966.tb52385.x

Cited by 61 publications

(21 citation statements)

References 31 publications

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“…Nowothy (1961) and Burton and Carter (1963) investigated chemical structure of lipid of endo toxins derived from gram-negative bacteria. Kasai andYamano (1964, 1966) alsa reported that thin-layer chromatography of lipid A showed more than 10 bands.…”

Section: Discussionmentioning

confidence: 99%

Symposium on Toxins

1972

JJMSB

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Section: Discussionmentioning

confidence: 99%

Symposium on Toxins

1972

JJMSB

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“…Several Gram-negative bacteria appear to have no KDO in their wall, indicating that the LPS may be different in its structure. Kasai (1966) has shown that the LPS of Bordetella species do not contain KDO. None of the wall samples or Gram-positive bacteria examined contained either KDO or sialic acid except for Arthrobacter globiforrnis NCIB 8907, which contained a small amount of KDO.…”

Section: Discussionmentioning

confidence: 99%

“…Sialic acid occurs either as a polyneuraminic acid (called colominic acid) in several bacterial species or as part of other heteropolysaccharides, such as the K antigen, in other bacterial species (for review see Luderitz, Jann & Wheat, 1968). A sensitive test for sialic acid is based on the formation of /3-formyl pyruvic acid upon periodate oxidation and the subsequent formation of a chromagen formed on heating the P-formyl pyruvate with thiobarbituric acid.…”

Section: Discussionmentioning

confidence: 99%

The Distribution of 2-Keto-3-deoxy-octonic Acid in Bacterial Walls

Ellwood

1970

Journal of General Microbiology

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“…for Pasteurella antigen; Davies, 1956;20 to 40pg./ml. with an Escherichia coli LPS preparation; Kasai, 1966).…”

Section: Discussionmentioning

confidence: 99%

The Somatic Antigens of Two Strains of Rhizobium trifolii

Humphrey¹,

Vincent²

1969

Journal of General Microbiology

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SUMMARYStrain-specific lipopolysaccharide extracted by hot phenol from two strains of Rhizobium trifolii was fully antigenic in rabbits with an injection schedule using Freund's adjuvant, and highly active in gel diffusion. A minor second diffusion line was considered to be due to a smaller fragment of the main molecule, since the antigen was converted almost entirely to this form by sodium dodecylsulphate and such disaggregated material absorbed antibodies to both forms of the antigen. Chemically the lipopolysaccharide showed some features related to those of the Enterobacteriaceae; for example, the presence of firmly bound lipid, 2-keto-3-deoxyoctonate, glucose, mannose, fucose and, in one strain, a heptose. However, the lipopolysaccharide of R. trifolii was unusual in its very low phosphorus content and the presence of glucuronic acid. One strain diverged even further in its high content of carbohydrate, its lack of a heptose, its anionic behaviour and the gelatinous nature of some preparations ; these properties might relate to the presence of a capsule-like structure which has been observed in old cultures of this strain.

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Chemical Studies on the Lipid Component of Endotoxin, With Special Emphasis on Its Relation to Biological Activities

Cited by 61 publications

References 31 publications

Symposium on Toxins

Symposium on Toxins

The Distribution of 2-Keto-3-deoxy-octonic Acid in Bacterial Walls

The Somatic Antigens of Two Strains of Rhizobium trifolii

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