Chemical sympathectomy alters food intake and thermogenic responses to catecholamines in rats

Villanueva, Iván; Piñón, Manuel; Quevedo-Corona, Lucı́a; Martı́nez-Olivares, Rubén; Racotta, Radu

doi:10.1016/s0024-3205(02)01739-3

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…In order to identify the trophic influence of sympathetic nerves, we prevented their development by treating rats with guanethidine from the first postnatal day until the age of 8-9 weeks. Despite delayed growth rate, sympathectomized rats did not demonstrate any alterations in behavior activity or health status, which is in line with previously reported data 43 . Chronic sympathetic denervation increased smooth muscle sensitivity of agonist-induced contraction.…”

Section: Discussionsupporting

confidence: 92%

Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation

Kostyunina

Zhang

Shvetsova

et al. 2020

Sci Rep

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Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl− channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium‐denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8–9-week age. Cl− substitution in the solution as well as Cl−-channel blockers (MONNA, DIDS) had larger suppressive effect on the methoxamine-induced arterial contraction and methoxamine-induced depolarization of smooth muscle cells in 1- to 2-week-old compared to 2- to 3-month-old rats. Vasculature of younger group demonstrated elevated expression levels of TMEM16A and bestrophin 3. Chronic sympathectomy increased Cl− contribution to arterial contraction in 2-month-old rats that was associated with an increased TMEM16A expression level. Our study demonstrates that contribution of Cl− channels to agonist-induced arterial contraction and depolarization decreases during postnatal development. This postnatal decline is associated with sympathetic nerves development.

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Section: Discussionsupporting

confidence: 92%

Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation

Kostyunina

Zhang

Shvetsova

et al. 2020

Sci Rep

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“…The effects in heart and liver were similar to those obtained in a previous experiment in which Gu was injected into neonatal rats 15.…”

Section: Discussionsupporting

confidence: 85%

“…Caloric intake is slightly reduced 11 , 14 . Recently we showed that neonatal rats treated with Gu show, as adults, a higher hypophagic response to intraperitoneal catecholamine administration, a lower resting oxygen consumption, and a higher respiratory quotient at rest, suggesting a lower rate of lipid use 15 . While both high-sucrose diet and D administration induces similar changes in glucose and lipid metabolism, the experimental manipulation of these two factors has opposite effects on the sympathetic nervous system activity: sucrose ingestion stimulates it 16 whereas D has been reported to suppress it 17 .…”

Section: Introductionmentioning

confidence: 97%

“…Because of our interest in the metabolic effects of sympathectomy 15 the aim of the present work was to assess in adult rats the effects of D on a background of a high-sucrose diet, on food intake, body weight, glucose tolerance, and lipid accumulation in the liver and muscle in rats treated with Gu. It was hypothesized that the poor food efficiency shown by D-treated rats 1 , 2 might be caused by an increase in energy expenditure perhaps due to an increase in sympathetic activity.…”

Section: Introductionmentioning

confidence: 99%

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The effects of sympathectomy and dexamethasone in rats ingesting sucrose

Franco-Colín¹,

Villanueva²,

Piñón³

et al. 2006

Int. J. Biol. Sci.

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Both high-sucrose diet and dexamethasone (D) treatment increase plasma insulin and glucose levels and induce insulin resistance. We showed in a previous work (Franco-Colin, et al. Metabolism 2000; 49:1289-1294) that combining both protocols for 7 weeks induced less body weight gain in treated rats without affecting mean daily food intake. Since such an effect may be explained by an increase in caloric expenditure, possibly due to activation of the sympathetic nervous system by sucrose ingestion, in this work, and using 10% sucrose in the drinking water, male Wistar rats were divided into 4 groups. Two groups were sympathectomized using guanethidine (Gu) treatment for 3 weeks. One of these groups of rats received D in the drinking water. Of the 2 groups not receiving Gu, one was the control (C) and the other received D. After 8 weeks a glucose tolerance test was done. The rats were sacrificed and liver triglyceride (TG), perifemoral muscle lipid, and norepinephrine (NE) levels in the liver spleen, pancreas, and heart were determined. Gu-treated rats (Gu and Gu+D groups) showed less than 10% NE concentration compared to C and D rats, less daily caloric intake and body-weight gain, more sucrose intake, and better glucose tolerance. The area under the curve after glucose administration correlated significantly with the mean body weight gain of the rats, except for D group. Groups D (D and Gu+D) also showed less caloric intake and body-weight gain but higher liver weight and TG concentration and lower peripheral muscle mass. The combination of Gu+D treatments showed some peculiar results: negative body weight gain, a fatty liver, and low muscle mass. Though the glucose tolerance test had the worst results for the D group, it showed the best results in the Gu+D group. There were significant interactions for Guan X Dex by twoway ANOVA test for the area under the curve in the glucose tolerance test, muscle mass, and muscle lipids. The results suggest that dexamethasone catabolic effect is not caused by sympathetic activation.

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“…Noradrenaline (NA), adrenaline and dopamine concentrations were determined according to the method of Villanueva et al ( 26 ) , using HPLC and an electrochemical detector (ESA Coulochem II). Briefly, BAT was homogenised (1:10, w/v) in ice-cold 0·4 m -perchloric acid and centrifuged, and the supernatant was frozen until catecholamine content determination.…”

Section: Methodsmentioning

confidence: 99%

Perinatal undernutrition programmes thyroid function in the adult rat offspring

et al. 2013

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Increasing evidence suggests that alterations in early nutrition programme physiological changes in adulthood. In the present study, we determined the effects of undernutrition during gestation and lactation on the programming of thyroid function in adult rat offspring. Perinatal undernutrition was achieved by a 40 % food restriction in female Wistar rats from the mating day to weaning. On postpartum day 21, the offspring of the control and food-restricted dams were weaned and given free access to a commercial diet until adulthood. The results showed that undernourished rats exhibited decreased 3,5,3 0 -triiodothyronine (T 3 ) levels but had normal thyroxine (T 4 ) and thyrotropin (TSH) levels at weaning; on day 90, these rats displayed a significant flip, exhibiting normalised T 3 (total and free) and total T 4 levels, but low free T 4 and persistently higher TSH levels, which were maintained even on postnatal day 140. This profile was accompanied by a scarce fat depot, a lower RMR and an exacerbated sympathetic brown adipose tissue (BAT) tone (deiodinase type 2 expression) in basal conditions. Moreover, when a functional challenge (cold exposure) was applied, the restricted group exhibited partial changes in TSH (29 v. 100 %) and T 4 (non-response v. 17 %) levels, a significant decrease in leptin levels (75 v. 32 %) and the maintenance of a sympathetic BAT over-response (higher noradrenaline levels) in comparison with the control group. The findings of the present study suggest that undernutrition during the perinatal period produces permanent changes in the hypothalamus -pituitary-thyroid axis with consequent low body weight and decreased RMR and facultative thermogenesis. We hypothesise that these changes predispose individuals to exhibiting adult subclinical hypothyroidism.

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Chemical sympathectomy alters food intake and thermogenic responses to catecholamines in rats

Cited by 7 publications

References 31 publications

Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation

Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation

The effects of sympathectomy and dexamethasone in rats ingesting sucrose

Perinatal undernutrition programmes thyroid function in the adult rat offspring

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