Chemical synthesis of <i>N</i>‐glycosylated insulin‐like androgenic gland factor from the freshwater prawn <i>Macrobrachium rosenbergii</i>

Katayama, Hidekazu; Nagasawa, Hiromichi

doi:10.1002/psc.3215

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…The CD spectrum of peptide 5 showed a negative band at 208 nm, which is typical of α‐helix. In addition, the whole spectral pattern of peptide 5 was similar to those of other insulin/relaxin family peptides reported previously 19,39–44 (Figure 4). The helix content of peptide 5 was estimated using BeStSel to be 16.1%.…”

Section: Resultssupporting

confidence: 84%

Chemical synthesis of insulin‐like peptide 1 and its potential role in vitellogenesis of the kuruma prawn Marsupenaeus japonicus

El-Desoky

Takeuchi

Katayama

et al. 2023

Journal of Peptide Science

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The insulin superfamily comprises a group of peptides with diverse physiological functions and is conserved across the animal kingdom. Insulin‐like peptides (ILPs) of crustaceans are classified into four major types: insulin, relaxin, gonadulin, and androgenic gland hormone (AGH)/insulin‐like androgenic gland factor (IAG). Of these, the physiological functions of AGH/IAG have been clarified to be the regulation of male sex differentiation, but those of the other types have not been uncovered. In this study, we chemically synthesized Maj‐ILP1, an ILP identified in the ovary of the kuruma prawn Marsupenaeus japonicus, using a combination of solid‐phase peptide synthesis and regioselective disulfide bond formation reactions. As the circular dichroism spectral pattern of synthetic Maj‐ILP1 is typical of other ILPs reported thus far, the synthetic peptide likely possessed the proper conformation. Functional analysis using ex vivo tissue incubation revealed that Maj‐ILP1 significantly increased the expression of the yolk protein genes Maj‐Vg1 and Maj‐Vg2 in the hepatopancreas and Maj‐Vg1 in the ovary of adolescent prawns. This is the first report on the synthesis of a crustacean ILP other than IAGs and also shows the positive relationship between the reproductive process and female‐dominant ILP.

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Section: Resultssupporting

confidence: 84%

Chemical synthesis of insulin‐like peptide 1 and its potential role in vitellogenesis of the kuruma prawn Marsupenaeus japonicus

El-Desoky

Takeuchi

Katayama

et al. 2023

Journal of Peptide Science

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“…The downregulation of genes involved in the insulin-like secretion pathway indicates that a PCD cascade effect primed the destruction of the AG and that this led to the inhibition of the production of the insulin-like hormone [ 6 , 35 , 65 , 66 , 67 ]. In fact, this androgenic hormone is normally secreted by the AG in males [ 68 ]. Importantly, this study shows that interactomic analyses permit us to track the activity of a cell-death compound in 5-day-old post-larvae; this will lead to a deeper understanding of its mechanism of action, enabling the knowledge to be applied for medical and ecological biotechnologies.…”

Section: Discussionmentioning

confidence: 99%

Molecular Approaches Detect Early Signals of Programmed Cell Death in Hippolyte inermis Leach

Glaviano,

Esposito,

Somma

et al. 2024

CIMB

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The protandric shrimp Hippolyte inermis is the only known marine invertebrate whose sex determination is strongly influenced by the composition of its food. In H. inermis, a sex reversal is triggered by the ingestion of diatoms of the genus Cocconeis associated with leaves of the seagrass Posidonia oceanica. These diatoms contain compounds that promote programmed cell death (PCD) in H. inermis and also in human cancer cells. Transcriptomic analyses suggested that ferroptosis is the primary trigger of the shrimp’s sex reversal, leading to the rapid destruction of the androgen gland (AG) followed by a chain of apoptotic events transforming the testes into ovaries. Here, we propose a molecular approach to detect the effects of compounds stimulating the PCD. An RNA extraction method, suitable for young shrimp post-larvae (five days after metamorphosis; PL5 stage), was established. In addition, six genes involved in apoptosis, four involved in ferroptosis, and seven involved in the AG switch were mined from the transcriptome, and their expression levels were followed using real-time qPCR in PL5 fed on Cocconeis spp., compared to PL5 fed on a basic control feed. Our molecular approach, which detected early signals of sex reversal, represents a powerful instrument for investigating physiological progression and patterns of PCD in marine invertebrates. It exemplifies the physiological changes that may start a few days after the settlement of post-larvae and determine the life destiny of an individual.

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“…Furthermore, N‐glycosylation was reported to affect peptide conformation in Maj ‐IAG (Katayama et al . 2014), but not in Mr ‐IAG (Katayama & Nagasawa 2019; Table 1; Fig. 3).…”

Section: Structure Of Iag Gene Chemical Property and Structure Of Iagmentioning

confidence: 95%

A sex‐reversing factor: insulin‐like androgenic gland hormone in decapods

Sun

2021

Reviews in Aquaculture

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A large number of decapod crustaceans with sexual dimorphism in body size, most of which, are important economic animals. Besides, some of them such as the red swamp crayfish Procambarus clarkii possess strong invasive ability and would damage ecosystem environment. Thereby, monosex culture of decapods would contribute to not only increasing yield and profits, but also reducing the risks of biological invasion. Insulin-like androgenic gland hormone was reported to be a key regulator in sex differentiation. All-male populations in Macrobrachium rosenbergii were successfully produced through insulin-like androgenic gland hormone manipulation. In addition to sex differentiation, insulin-like androgenic gland hormone was reported to be involved in male secondary sex characteristics maintenance, growth, metabolism, morphology change, ovary development and other functions. Considering its important application value for decapods, insulin-like androgenic gland hormone has attracted much attention in recent years. This hormone was reported to have the structure and function similar to androgenic gland hormone of isopods. In this study, the origin of name of androgenic gland hormone and insulin-like androgenic gland hormone was investigated, and the recent research on structure, function, application and molecular regulation mechanism of insulin-like androgenic gland hormone of decapods was reviewed.

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Chemical synthesis of N‐glycosylated insulin‐like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii

Cited by 7 publications

References 39 publications

Chemical synthesis of insulin‐like peptide 1 and its potential role in vitellogenesis of the kuruma prawn Marsupenaeus japonicus

Chemical synthesis of insulin‐like peptide 1 and its potential role in vitellogenesis of the kuruma prawn Marsupenaeus japonicus

Molecular Approaches Detect Early Signals of Programmed Cell Death in Hippolyte inermis Leach

A sex‐reversing factor: insulin‐like androgenic gland hormone in decapods

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