Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells

Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy

“…The resulting HE also expressed SOX17 ( Figures S1B – S1D ), a transcription factor expressed in AGM vascular structures and required for HSPC generation ( Clarke et al, 2013 ; Kim et al, 2007 ; Ng et al, 2016 ). Transforming growth factor beta (TGFβ) inhibitor SB431542 (SB) significantly promoted EHT for generation of CD45+CD43+ HSPCs ( Chang et al, 2022 ) that co-expressed definitive markers CD44 ( Fidanza et al, 2019 ; Oatley et al, 2020 ) and RUNX1c ( Ng et al, 2016 ) ( Figures S1E – S1H ). The resulting HSPCs also maintained a high viability after freeze thaw ( Figures S1I and S1J ).…”

Temporal Expression of Transcription FactorID2Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells

“…To induce hematopoiesis, 50 ng/mL SCF and FLT3L were used in Stemline-II medium from day 4 to day 12. 34 Floating day 12 hematopoietic stem and progenitor cells (HSPCs) were collected and treated with 50 ng/mL SCF, FLT3L, IL-3, IL-7, and IL-15 from day 15 to day 23. From day 23 to day 30, differentiated cultures were treated with 50 ng/mL SCF, FLT3L, IL-7, and IL-15 as well as 5 μg/mL heparin.…”

“…Briefly, 6 μM CHIR99021 was used to induce mesoderm differentiation from day 0 to day 2 in LaSR basal medium, followed by 10 μM SB431542, 50 ng/mL SCF, and VEGF treatment from day 2 to day 4. To induce hematopoiesis, 50 ng/mL SCF and FLT3L were used in Stemline-II medium from day 4 to day 12 . Floating day 12 hematopoietic stem and progenitor cells (HSPCs) were collected and treated with 50 ng/mL SCF , FLT3L , IL-3 , IL-7 , and IL-15 from day 15 to day 23.…”

Chemically defined generation of human definitive hematopoietic stem and progenitor cells

“…For additional details on hPSC differentiation into hemogenic endothelium ( Figures 2 A and 2B) and HSPCs ( Figures 3 A–3C), please refer to Chang et al. 1 , 2 We have successfully performed HSPC differentiation in 10 hPSC lines, including H1, H9, and H13 human embryonic stem cell (hESC) lines, as well as 6-9-9 and 19-9-11 human induced pluripotent stem cell (hiPSC) lines. We expect similar differentiation results in other hPSC lines.…”