Chemically defined media for commercial fermentations

Zhang, J.; Greasham, Randolph

doi:10.1007/s002530051411

Cited by 177 publications

(117 citation statements)

References 2 publications

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“…Because the synthetic medium is usually composed of known amounts of pure chemicals, it is possible to determine the specific requirements for growth and product formation by systematically adding or eliminating components from the formulation, which also results in more stability and reproducible culture conditions [8]. A synthetic medium is thus well suited for fundamental studies of microbe metabolism and fermentation optimization.…”

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confidence: 99%

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Dong

Zhao

et al. 2012

Chin. Sci. Bull.

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A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium (S2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recombinant Z. mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.recombinant Zymomonas mobilis, synthetic medium, ethanol production, response surface methodology, trisodium citrate, xylose Citation:Dong H N, Zhao X M, Ma Y Y, et al. Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology.

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confidence: 99%

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Dong

Zhao

et al. 2012

Chin. Sci. Bull.

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“…Chemically defined media are generally known to produce slower growth and low protein titres than the complex media 33 . Nonetheless, use of chemically defined media in producing recombinant proteins is a common practice [60][61][62][63] because these media attain more consistent titres, allow easier process control and monitoring, and simplify downstream recovery of the target protein.…”

Section: Development Of Growth Mediamentioning

confidence: 99%

High Yield Production of Heterologous Proteins with Escherichia coli

Tripathi

2009

DSJ

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“…d Electrophoretic analysis of native gels stained for activity using x-gal; Lane 1 negative control-crude extract from E. coli MC1061 pProEx-HTb; Lane 2 crude extract from E. coli MC1061 pProEx-HTb_lacZ-HT. Arrow points to the expressed recombinant protein option and reduces extensive testing of raw materials (Zhang and Greasham 1999;Tripathi et al 2009). …”

Section: Media Analysismentioning

confidence: 99%

Minimization of phosphorus in the fermentation media of Escherichia coli producing a model recombinant protein

Witt

O’Dwyer

Walsh

2014

Int. J. Environ. Sci. Technol.

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In the biotechnology sector, a main processing goal is the production of high cell (and hence product) yields. Therefore, little consideration is traditionally given to the potential environmental impacts of excess culture media ingredients. This study investigated the scope for reducing the quantities of phosphorus (P) present in both a complex terrific broth (TB) and semi-defined minimal media 9/yeast extract (M9/YE) fermentation media used to culture a model Escherichia coli strain engineered to produce a recombinant b-galactosidase. Reductions of up to 70 % did not adversely affect biomass yields attained; however, further P minimization leads to a drop in dry cell weight obtained, particularly in the case of semi-defined media. P concentration in TB media had little effect upon total recombinant protein expression levels achieved. In the case of M9/YE media, reductions [70 % P negatively affected product expression levels. Protein functionality, assessed by k m and V max , was not influenced by the type of media nor the P concentration present. Overall, the results indicate that P can be reduced by a minimum of 70 % without adversely affecting the biomass yield, the recombinant protein yield or functionality. Such reductions should lead to significant P savings in the large-scale manufacturing of proteins produced by genetic engineering in E. coli.

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Chemically defined media for commercial fermentations

Cited by 177 publications

References 2 publications

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

High Yield Production of Heterologous Proteins with Escherichia coli

Minimization of phosphorus in the fermentation media of Escherichia coli producing a model recombinant protein

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