Chemically Induced Mutants of &amp;lt;i&amp;gt;Brassica oleracea var. botrytis&amp;lt;/i&amp;gt; Maintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation

Hadi, Fazal; Fuller, Michael P.

doi:10.4236/ajps.2013.43063

Cited by 12 publications

(3 citation statements)

References 28 publications

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“…The assessment of drought resistance under different treatments was carried out by full leaf assays [37]. Distilled water with different test concentrations of polyethylene glycol (PEG 6000) 0, 5, 10, and 15% denoted by T 0 , T 1 , T 2 , and T 3 , respectively was prepared and sterilized.…”

Section: Relative Water Contentmentioning

confidence: 99%

Straw Incorporation in Contaminated Soil Enhances Drought Tolerance but Simultaneously Increases the Accumulation of Heavy Metals in Rice

et al. 2022

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Heavy metals (HMs) and drought stress are worldwide issues of concern because of their adverse effects on the growth and productivity of rice. Straw burning causes air pollution via greenhouse gas (GHG) emissions and it requires sustainable management. The introduction of HMs into the food chain poses a major health risk to humans. In this regard, straw incorporation into the soil could reduce air pollution and drought stress. However, its simultaneous impact on HMs’ uptake and drought stress tolerance in crops is unknown. Therefore, the present study aimed to investigate the impact of rice straw incorporation in soil on HMs (Cd, Cu, Pb, and Fe) availability, accumulation, and drought stress tolerance in rice (Oryza sativa L.) grown in pots under glasshouse conditions. The soil samples were collected from a non-contaminated agricultural field (control) and the contaminated field, irrigated with industrial effluent and treated with straw. Straw (1% w/w) was mixed in soils and control plants without straw application were grown under both contaminated and normal soil conditions. The results showed that straw incorporation in soils significantly enhanced the accumulation of HMs in rice grain and other vegetative parts of rice as compared to control. Moreover, straw application harmed chlorophyll and carotenoids. Straw application significantly increased proline in leaves (274.0 µg mL−1) as compared to the control (166.8 µg mL−1). Relative water contents were higher in straw-treated plants, thereby increasing drought stress tolerance. Straw application increased the accumulation of HMs and consequently reduced the biomass of the plant. In conclusion, straw incorporation enhanced drought stress tolerance but simultaneously elevated the accumulation of HMs under contaminated soil in Oryza sativa L.

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Section: Relative Water Contentmentioning

confidence: 99%

Straw Incorporation in Contaminated Soil Enhances Drought Tolerance but Simultaneously Increases the Accumulation of Heavy Metals in Rice

et al. 2022

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“…The literature on obtaining a new variety with an increased tolerance through conventional breeding approach is scarce (TAHIRA et al, 2006). Traditional approaches are limited by the complexity of stress tolerance traits, low genetic variation and field components under stress, and lack of efficient selection techniques (BHUTTA & HANIF, 2013;HADI & FULLER, 2013). Moreover, it happens that the resistance to salt stress is subject to changes during plant growth.…”

Section: Discussionmentioning

confidence: 99%

BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture

Krupa-Małkiewicz

Bienias

2018

Cienc. Rural

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In this study, we performed BSA to identify genetic markers linked to salt tolerance. We tested the genetic diversity among four bulked DNA samples of EMS induced mutant clones and one bulked DNA sample of non-mutated clone of Petunia for salt tolerance in in vitro callus cultures using RAPD and ISSR markers. Out of the 36 RAPD and 16 ISSR primers identified, 25 and 13 were effectively used to amplify genomic DNA of all the five bulked samples, respectively. In total, 114 RAPD amplifications products were obtained, of which 28% were polymorphic and 2% were genotype-specific bands. Out of the 64 ISSR amplification products obtained, 51% were polymorphic and 1% was genotype-specific bands. Results of this study indicated the existence of two patterns of distorted segregation among the studied markers. The first one indicates the differences between non-mutated clones of Petunia and its putative mutants. The second one was observed only between putative mutants and putative mutants tested for salt tolerance in in vitro culture. Both RAPD and ISSR analysis successfully detected the association with changes induced by chemical mutagenesis and salinity. Furthermore, our results indicate that BSA method can be useful in the rapid detection of molecular markers for further marker-assisted selection.

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“…Novel genetic variation not present in domesticated crops can also be generated through chemical or radiative mutagenesis. This approach has been used to investigate traits including drought and salt stress resilience in cauliflower (for example, Fuller et al, 2006;Hadi and Fuller, 2013). In addition, genetic mapping populations that segregate for stress resilience traits can also be screened to locate Quantitative Trait Loci (QTLs), enabling the determination of chromosomal regions linked to traits of interest.…”

Section: Generating Resilient Linesmentioning

confidence: 99%

Addressing the threat of climate change to agriculture requires improving crop resilience to short-term abiotic stress

et al. 2018

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Climate change represents a serious threat to global agriculture, necessitating the development of more environmentally resilient crops to safeguard the future of food production. The effects of climate change are appearing to include a higher frequency of extreme weather events and increased day-to-day weather variability. As such, crops which are able to cope with short-term environmental stress, in addition to those that are tolerant to longer term stress conditions are required . It is becoming apparent that the hitherto relatively little studied process of post-stress plant recovery could be key to optimizing growth and production under fluctuating conditions with intermittent transient stress events. Developing more durable crops requires the provision of genetic resources to identify useful traits through the development of screening protocols. Such traits can then become the objective of crop breeding programmes. In this study, we discuss these issues and outline example research in leafy vegetables that is investigating resilience to short-term abiotic stress.

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Chemically Induced Mutants of <i>Brassica oleracea var. botrytis</i> Maintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation

Cited by 12 publications

References 28 publications

Straw Incorporation in Contaminated Soil Enhances Drought Tolerance but Simultaneously Increases the Accumulation of Heavy Metals in Rice

Straw Incorporation in Contaminated Soil Enhances Drought Tolerance but Simultaneously Increases the Accumulation of Heavy Metals in Rice

BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture

Addressing the threat of climate change to agriculture requires improving crop resilience to short-term abiotic stress

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