Chemically Modified Silk Proteins

Chen, Jianming; Venkatesan, Harun; Hu, Jinlian

doi:10.1002/adem.201700961

Cited by 52 publications

(44 citation statements)

References 103 publications

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“…Coupling reactions are mainly used to immobilize peptides, molecules and polymers in fiber proteins. Copper-catalyzed azide-alkyne cycloaddition reactions, cyanuric chloride, carbodiimide and glutaraldehyde are very effective coupling agents [58,59]. The amino acid modifications are made through arginine masking, which is used to regulate the surface charge, sulfation/oxidation of tyrosine, which causes the hydrolysis of the fiber protein [58], and azo-modified tyrosine that can be used to install small molecules into fiber protein, resulting in hydrophobic and hydrophilic derivatives [60].…”

Section: Chemical Treatments In Animal Fibersmentioning

confidence: 99%

Biofunctionalization of Natural Fiber-Reinforced Biocomposites for Biomedical Applications

et al. 2020

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In the last ten years, environmental consciousness has increased worldwide, leading to the development of eco-friendly materials to replace synthetic ones. Natural fibers are extracted from renewable resources at low cost. Their combination with synthetic polymers as reinforcement materials has been an important step forward in that direction. The sustainability and excellent physical and biological (e.g., biocompatibility, antimicrobial activity) properties of these biocomposites have extended their application to the biomedical field. This paper offers a detailed overview of the extraction and separation processes applied to natural fibers and their posterior chemical and physical modifications for biocomposite fabrication. Because of the requirements for biomedical device production, specialized biomolecules are currently being incorporated onto these biocomposites. From antibiotics to peptides and plant extracts, to name a few, this review explores their impact on the final biocomposite product, in light of their individual or combined effect, and analyzes the most recurrent strategies for biomolecule immobilization.

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Section: Chemical Treatments In Animal Fibersmentioning

confidence: 99%

Biofunctionalization of Natural Fiber-Reinforced Biocomposites for Biomedical Applications

et al. 2020

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“…Silk sutures have been FDA‐approved for use in surgery, and silk materials have been developed as effective extracellular matrix analogs for cell growth and direction . Silk can be chemically modified in a variety of ways to produce materials with additional functionalities, including with azobenzene functional groups, via the tyrosine residues on silk fibroin that can be chemically modified to form azobenzene derivatives on the silk through diazonium coupling ( Figure a) . 5.3% of the amino acids composing silk fibroin are tyrosine, which is distributed through the silk fibroin protein sequence, and this azobenzene postmodified silk fibroin is then referred to as “azosilk” or “optosilk.” This diazonium coupling method, developed by Murphy and Kaplan, can be performed in mild conditions and results in a relatively high conversion of tyrosine residues to azobenzene residues (≈40%) .…”

Section: Interfacing Azo To Bio: An Eye Toward Influencing Living Sysmentioning

confidence: 99%

Photoreversible Soft Azo Dye Materials: Toward Optical Control of Bio‐Interfaces

Chang

Fedele

Priimägi

et al. 2019

Advanced Optical Materials

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systems interact with artificial materials, researchers have now assembled a versatile toolkit of materials and processes that allows one to fine-tune interactions at the interface, tailoring specific biological responses on demand. These strategies for biocontrol can be broadly categorized as chemical (charge, ligand presence, surface groups), material changes (moisture content, stiffness), or morphological changes (molecular orientation, surface topography, or mechanical actuation). Rational design of materials for biological interface applications has a unique set of challenges due to the unique requirements of a wide range of biological systems, since different tissues present specific compositions, with their own elasticity, structural organization, and triggering mechanisms. Even within a single organ or tissue, mechanical properties can vary widely depending on the region. A recent study of viscoelasticity in live mouse brain tissue for example found a tenfold difference within the brain depending on the region and morphological structure studied. [2] However, most biological tissues are far less stiff, and far more viscoelastic than typical artificial materials employed at their interface, especially early artificial cell culture materials, which can be much stiffer than in vivo extracellular matrix by many orders of magnitude. [3] In vivo, cells interface with a surrounding microenvironment consisting of an intricate macromolecular network of proteins and sugars swollen in an aqueous media gel. Therefore, the interaction between cells and the extracellular matrix (ECM) in the body is complex and involves a variety of cues related to topography, chemical markers, protein composition, solubility factors, and mechanical properties such as stiffness and elasticity (Figure 1a). [4] Yet much research over the past decades was focused on studying in vitro the effects of each of these signals on cell behavior, with a particular interest on the chemical factors, whereas only recently more advanced biomaterials with controlled physicomechanical properties have been developed, such as those with tunable and variable stiffness, alignment and orientation of key functional groups, and mechanical actuation. There is a large range of stiffness in human tissue, where bone (≈100 GPa) is nine orders of magnitude harder than brain tissue (≈0.1 kPa). [4] Therefore, biomaterial researchers assume a complex task of providing materials and processing technologies for controlling stiffness and micro-and nanostructures in Photoreversible optically switchable azo dye molecules in polymer-based materials can be harnessed to control a wide range of physical, chemical, and mechanical material properties in response to light, that can be exploited for optical control over the bio-interface. As a stimulus for reversibly influencing adjacent biological cells or tissue, light is an ideal triggering mechanism, since it can be highly localized (in time and space) for precise and dynamic control over a biosystem, and low-power visible light...

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“…Due to the nature of silk fibroin is degraded by enzymes, controlling the availability and content of enzymes becomes very important in managing the degradation rate [87,76,85]. It has also been reported that degradation behavior can be controlled through chemical modification [88][89][90].…”

Section: Factors Affecting Silk Fibroin Degradationmentioning

confidence: 99%

“…The degradation behavior of silk fibroin is often facilitated by other object responses [111,112], and does not lead to an immunogenic response. In many literatures, degradation of silk fibroin is reported to be enzymatically degraded [113][114][115]89] because of the dominant role of enzymes in the degradation process of silk fibroin. Based on this fact, the enzymatic degradation process can be divided into two steps, namely enzyme adsorption on the substrate surface, and ester bond hydrolysis [115,89].…”

Section: Degradation Behavior By Enzymesmentioning

confidence: 99%

“…In many literatures, degradation of silk fibroin is reported to be enzymatically degraded [113][114][115]89] because of the dominant role of enzymes in the degradation process of silk fibroin. Based on this fact, the enzymatic degradation process can be divided into two steps, namely enzyme adsorption on the substrate surface, and ester bond hydrolysis [115,89]. Silk fibroin is very sensitive to degradation by proteolytic enzymes such as carboxylase and actinase [114,85,116].…”

Section: Degradation Behavior By Enzymesmentioning

confidence: 99%

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Degradation Behavior of Silk Fibroin Biomaterials - A Review

Purnomo¹,

Setyarini²,

Sulistyaningsih³

2019

JESTR

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Silk fibroin-based biomaterials have developed widely for biomedical applications including soft tissue engineering and bone implants. Controlling the degradation rate is very notable in maintaining the performance of biomaterials in carrying out their functions. This review was focused on the process of biodegradation of silk fibroin and its controllers including among other factors that influence the degradation process, mechanisms, behavior, the role of ultraviolet (UV) radiation, and the effect of molecular weight. All studies in this paper provide more in-depth knowledge about the biodegradation of silk fibroin enzymatically as well as UV radiation effect.

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Chemically Modified Silk Proteins

Cited by 52 publications

References 103 publications

Biofunctionalization of Natural Fiber-Reinforced Biocomposites for Biomedical Applications

Biofunctionalization of Natural Fiber-Reinforced Biocomposites for Biomedical Applications

Photoreversible Soft Azo Dye Materials: Toward Optical Control of Bio‐Interfaces

Degradation Behavior of Silk Fibroin Biomaterials - A Review

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