Chemico-Biological Profiling of Blumea lacera (Burm.f.) DC. (Family: Asteraceae) Provides New Insights as a Potential Source of Antioxidant, Cytotoxic, Antimicrobial, and Antidiarrheal Agents

Abstract

Blumea lacera (Burm.f.) DC., popular for its traditional use in different diseases, was employed in phytochemical and biological investigations. The chemical studies led to the isolation of acyclic diterpene-phytol (1) along with two fatty acids-linolenic acid (2) and oleic acid (3). All the structures were determined by 1H NMR spectroscopic analysis and first time reported from this plant. Different fractions of crude methanol extract were subjected to antioxidant, cytotoxicity, antimicrobial, and antidiarrheal assays. The molecular docking studies have been implemented using PyRx, UCSF Chimera, Discovery Studio, and online tools. In addition, The ADME/T analysis and PASS prediction were implemented by using PASS online tools. In the molecular docking study of antioxidant, cytotoxicity, antimicrobial, and antidiarrheal activity, the compounds showed strong binding affinity ranging from −4.5 to −6.2 kcal/mol. Again, all three isolated compounds met the preconditions of Lipinski’s five rules for drug discovery. In DPPH free radical scavenging assay, the pet-ether and chloroform soluble fraction showed noteworthy antioxidant activity sowing promising IC50 values (10.76 μg/ml and 11.77 μg/ml, respectively), compared to the standard (6.05 μg/ml) with a total phenolic content range of 7.33–40.33 mg of GAE/gm. The pet-ether soluble fraction revealed substantial cytotoxicity showing an LC50 value of 1.03 μg/ml, compared to the standard (0.93 μg/ml). Besides, ethyl acetate soluble fraction showed moderate activity against both Gram-positive and Gram-negative bacteria, while both ethyl acetate and pet-ether soluble fraction showed excellent dose-dependent antidiarrheal activity.

The amount of antioxidants in honey has a strong relationship with the plants selected by honey bees

“…Meanwhile, some plants specifically contain more antioxidants 24 . Plants have pharmacological effects on humans due to the production of some chemical substances, among these compounds are phenolic compounds, alkaloids and flavonoids, which are also known as secondary metabolites 25 , 26 . Medicinal plants can be used in the prevention or treatment of some diseases due to their antioxidant and phenolic compounds 27 .…”

Phytochemical and Biological Investigation of an Indigenous Plant of Bangladesh, Gynura procumbens (Lour.) Merr.: Drug Discovery from Nature

“…The experiment was conducted in accordance with the FELSA (Federation of European Laboratory Animal Science Associations) criteria. All of the studies were carried out in accordance with the institutional ethical committee’s approved procedures for the maintenance and utilization of laboratory animals [ 72 ]. The Ethical Review Committee of the Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh, issued its consent under the approval number Fa.Ph.E/002-A/22 prior to performing animal investigations.…”

“…To examine whether the plant extracts might scavenge free radicals from 1,1-diphenyl-2-picrylhydrazyl (DPPH), a total of 2.0 mL of a plant extract solution at various concentrations (ranging from 500 g/mL to 0.977 g/mL) was combined with 3.0 mL of a DPPH methanol solution (20 g/mL). The antioxidant activities of the plant extract were evaluated by comparing its ability to decolorize a purple-colored DPPH methanol solution to that of ascorbic acid (ASA) [ 72 , 73 , 74 , 75 , 76 , 77 ]. …”

“…With DMSO serving as the negative control, vincristine sulfate was employed as the standard for comparison in a range of doses (400 g/mL to 0.78125 g/mL). After counting the nauplii visually, 5 mL of simulated seawater was added to the mixture in vials [ 72 , 74 , 76 , 77 ]. …”

Green Synthesis, Characterization, and Assessment of Iron Oxide Nanoparticles' Antibacterial Activity Using Blumea lacera Root Extract

“…In India's Konkan region, the herb is utilized to ward against parasites like fleas [8][9][10]. In the homeopathic system, it is prescribed for headaches, enuresis, neuralgia, and coughing brought on by colds [11][12][13][14]. The current study looked into the environmentally friendly production and characterization of iron and copper oxide nanoparticles using root extract from Blumea lacera, and evaluated the antibacterial efficacy of these nanoparticles against bacterial infections in humans.…”