Chemico-physical characterization of gelatin films modified with oxidized alginate

Boanini, Elisa; Rubini, Katia; Panzavolta, Silvia; Bigi, Adriana

doi:10.1016/j.actbio.2009.06.015

Cited by 147 publications

(84 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction of the triple helix content revealed by DSC and XRD results is similar to that observed on crosslinked gelatin, where the degree of renaturation of the protein decreases on increasing the degree of crosslinking [6,10]. It can be suggested that the interaction of the oxygen-rich groups on the GO surface with gelatin chains during gelling interferes with gelatin renaturation and reduces the extent of triple helix content, in agreement with previous studies [16].…”

Section: Gelatin-go Filmssupporting

confidence: 79%

Structural reinforcement and failure analysis in composite nanofibers of graphene oxide and gelatin

Panzavolta

Bracci

Gualandi

et al. 2014

Carbon

View full text Add to dashboard Cite

In this work we study the mechanical properties and failure mechanism of nanocomposites made of graphene oxide sheets embedded in polymeric systems, namely films and electro-spun nanofibers. In this last system, contrary to conventional bulk composites, the size of the nano-reinforcement (GO sheets) is comparable to the size of the nanofibers to be reinforced (≈ 200 nm). As an ideal polymeric matrix we use gelatin.We demonstrate that the high chemical affinity of the two materials hinders the renaturation of gelatin into collagen and causes a nearly ideal mixing in the GO-gelatin composite. Adding just 1% of GO we obtain an increase of Young's modulus >50% and an increase of fracture stress >60%. We use numerical simulations to study the failure mechanism of the fibers. Calculations agree very well with experimental data and show that, even if cracks start at GO sheet edges due to stress concentrations, crack propagation is hindered by the nonlinear behaviour of the matrix. As an additional advantage, the presence of the GO sheets in continuous gelatin films improves the material stability to phosphate buffer solutions from 2 days to 2 weeks, making it a better material than gelatin for applications in biological environments.4

show abstract

Section: Gelatin-go Filmssupporting

confidence: 79%

Structural reinforcement and failure analysis in composite nanofibers of graphene oxide and gelatin

Panzavolta

Bracci

Gualandi

et al. 2014

Carbon

View full text Add to dashboard Cite

show abstract

“…3,21,31,32 Briey, 5 g of alginate were dispersed in 25 ml ethanol (VWR int., Belgium) and 1.605 g of sodium metaperiodate were dissolved in 25 ml deionized (DI) water to get 7.5 mmol periodate solution. The periodate solution was slowly added to the sodium alginate dispersion, which was continuously stirred under dark conditions at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The reduction of gelatin release on increasing gelatin concentration in ADA-GEL microcapsules might be explained by the increasing extent of physical as well as chemical crosslinking in this material. 3 In both buffer solutions, namely HBSS and TBSS, the percentage of released gelatin was found to be very low which means that a signicant quantity of gelatin remained in the microcapsules that could enhance cell-material interactions due to the presence of the cell adhesive peptide, RGD (Arg-Gly-Asp) sequence, in gelatin. 56,57 This result thus indicates that the present ADA-GEL microcapsules could be very promising for tissue engineering applications.…”

Section: Gelatin Releasementioning

confidence: 99%

“…26 However, gelatin's poor mechanical properties, which limit its wider possible applications, can be overcome by covalent crosslinking with ADA. 3 Therefore the main goal of this investigation was the synthesis of the alginate-gelatin crosslinked hydrogel (ADA-GEL) aiming to fabricate microcapsules. To the best of our knowledge, this is the rst study reporting the fabrication and characterization of microcapsules from ADA-GEL.…”

Section: 18mentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

et al. 2014

View full text Add to dashboard Cite

Microencapsulation of cells by using biodegradable hydrogels offers numerous attractive features for a variety of biomedical applications including tissue engineering. This study highlights the fabrication of microcapsules from an alginate-gelatin crosslinked hydrogel (ADA-GEL) and presents the evaluation of the physico-chemical properties of the new microcapsules which are relevant for designing suitable microcapsules for tissue engineering. Alginate di-aldehyde (ADA) was synthesized by periodate oxidation of alginate which facilitates crosslinking with gelatin through Schiff's base formation between the free amino groups of gelatin and the available aldehyde groups of ADA. Formation of Schiff's base in ADA-GEL and aldehyde groups in ADA was confirmed by FTIR and NMR spectroscopy, respectively. Thermal degradation behavior of films and microcapsules fabricated from alginate, ADA and ADA-GEL was dependent on the hydrogel composition. The gelation time of ADA-GEL was found to decrease with increasing gelatin content. The swelling ratio of ADA-GEL microcapsules of all compositions was significantly decreased, whereas the degradability was found to increase with the increase of gelatin ratio. The surface morphology of the ADA-GEL microcapsules was totally different from that of alginate and ADA microcapsules, observed by SEM. Two different buffer solutions (with and without calcium salt) have an influence on the stability of microcapsules which had a significant effect on the gelatin release profile of ADA-GEL microcapsules in these two buffer solutions.

show abstract

“…The result is essentially an open network formed by the association of the chains in the junction zones, rich in amino groups, strengthened by regions in which the helical structure of collagen has been reconstituted (Wong, 1995). According to Boanini et al (2010), an increase in triple helix structures may be related to an increase in the thermal stability of gelatin gels. …”

Section: X-ray Diffraction Of Immobilization Matricesmentioning

confidence: 99%

Influence of bloom number and plastifiers on gelatin matrices produced for enzyme immobilization

Kempka

Souza

et al. 2014

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-In this study the immobilization of lipase in matrices produced with gelatin with different Bloom values and with the addition of plastifiers was investigated to evaluate the influence of the Bloom value, as well as the capacity of the plastifiers to maintain the enzyme immobilized and the immobilization yield. The results indicated the need for crosslinking of the matrices with glutaraldehyde due to the high solubility in water, explained by the amino acid profile, which confirms the solubility of gelatin. Mannitol showed greater efficiency in the lipase immobilization, since it led to more porous structures and more uniform pores. These structures were also influenced by the gelatin concentration; greater concentrations associated with intermediate concentrations of plastifier led to matrices with a greater immobilization yield (87.92%). The X-ray diffraction analysis revealed that the structure of the immobilization matrices was partially crystalline.

show abstract

Chemico-physical characterization of gelatin films modified with oxidized alginate

Cited by 147 publications

References 31 publications

Structural reinforcement and failure analysis in composite nanofibers of graphene oxide and gelatin

Structural reinforcement and failure analysis in composite nanofibers of graphene oxide and gelatin

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

Influence of bloom number and plastifiers on gelatin matrices produced for enzyme immobilization

Contact Info

Product

Resources

About