Chemiluminescence analysis of the activity of Pecten maximua hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms

Gall, G Le; Bachère, Evelyne; Mialhe, Eric

doi:10.3354/dao011181

Cited by 47 publications

(32 citation statements)

References 23 publications

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“…Hemocytes from many bivalve species are known to be able to produce ROS. Oxidative mechanisms were reported in the oysters C. virginica, C. gigas, C. ariakensis and O. edulis (Larson et al, 1989;Bachère et al, 1991;Hégaret et al, 2003b;Lambert et al, 2003;Goedken and DeGuise, 2004;Labreuche et al, 2006;Donaghy et al, 2009), the mussels M. edulis and M. galloprovincialis (Pipe, 1992;Carballal et al, 1997b;Ordás et al, 2000b) and the scallops P. maximus and C. farreri (Le Gall et al, 1991;Chen et al, 2007a,b). In clams, few data are currently available and contradictory results between species were reported.…”

Section: Oxidative Mechanismsmentioning

confidence: 99%

“…In vertebrates, phagocytosis of foreign material or chemical stimulation was shown to induce an increase in ROS production (Bedard and Krause, 2007;Nauseef, 2008;Segal, 2008). In bivalve species, phagocytosis of zymosan particles increased oxidative mechanisms in the oysters C. gigas , C. virginica (Hégaret et al, 2003b), O. edulis , the clam species S. patula (Greger et al, 1995), M. mercenaria (Buggé et al, 2007), as well as in the pectinid P. maximus (Le Gall et al, 1991). Activation with chemicals such as phorbol myristate acetate (PMA) also increased ROS production in C. virginica (Goedken and DeGuise, 2004) whereas, PMA exposure resulted in a decrease in ROS in C. gigas hemocytes .…”

Section: Oxidative Mechanismsmentioning

confidence: 99%

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Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): Current knowledge and future prospects

et al. 2009

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: Hemocytes are the immune effectors in clams, directing cellular but also humoral defences. In the Manila clam (Ruditapes philippinarum) and the carpet shell clam (Ruditapes decussatus), two phenotypically similar venerid species, fishery and aquaculture importance motivated scientific studies to improve knowledge about the participation of hemocytes in immune response. Currently, gaps still persist concerning the classification of hemocyte sub-populations as well as their non-immune activities. This review summarizes current knowledge about hemocytes of the Manila clam and the carpet shell clam, suggests some probable misinterpretations arising from extrapolations from other bivalves and vertebrates, and suggests future research prospects. The susceptibility of hemocytes to environmental modifications as well as biological and chemical toxicants is also re-evaluated. Hemocytes of bivalves are not limited in function to immunity and should be considered in a wider scheme including the environment and whole-animal physiology.

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Section: Oxidative Mechanismsmentioning

confidence: 99%

Section: Oxidative Mechanismsmentioning

confidence: 99%

Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): Current knowledge and future prospects

et al. 2009

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“…Adaptations for avoiding or bypassing the haemocytic oxidative microbicidal system have been demonstrated in several pathogens, using in vitro host-pathogen models with isolated Ž . specific pathogens Hervio et al, 1989;Le Gall et al, 1991;Volety and Chu, 1995 . In crustaceans, the demonstration of respiratory burst is quite recent. Using the Ž .…”

Section: Introductionmentioning

confidence: 99%

Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus vannamei

et al. 2000

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Ž. A spectrophotometric nitroblue tetrazolium NBT reduction assay was used to demonstrate the Ž y . production of superoxide anions O by haemocytes of the white shrimp Penaeus Õannamei. It 2 was found that haemocytes, without receiving an experimental stimulant, showed a rather high Ž background activity. Therefore, optimal parameters number of haemocytes, type of incubation . medium, type and concentration of stimulants were first established, in order to obtain a reliable and reproducible quantitative assay. With this optimized assay, and using specific inhibitors, it was shown that it is indeed the production of O y that was measured. Activities varied strongly 2 among individual shrimp specimens. Live bacteria, among these Vibrio strains, induced O y 2 production in the haemocytes, in a dose-dependent manner. Whereas Vibrio anguillarum and a probiotic strain of V. alginolyticus evoked clear reactions, a pathogenic strain of V. harÕeyi failed to elicit O y production in the haemocytes. It is discussed that this may explain this strain's 2 capability of evading the host's oxidative microbicidal activity, which would be a virulence factor in these bacteria. Heat-killed bacteria hardly induced NBT reduction in the cells. The fungicide propiconazole or Tilt w , found as a pollutant in the aquatic environment where the shrimp are reared, was tested for its effect on NBT reduction by the haemocytes. In haemocytes that did not receive an experimental stimulant, Tilt w induced the reduction of NBT in a dose-dependent manner. In experimentally stimulated haemocytes, however, Tilt w strongly reduced the reaction upon the stimulant PMA. Probable explanations for these seemingly controversial effects of Tilt w are discussed, as are possible consequences of this sort of pollutants for shrimp aquaculture. This easy to perform and relatively cheap and simple quantitative assay for measuring the activity of an oxidative microbicidal mechanism in shrimp haemocytes, appears quite reliable and may therefore prove to be a valuable tool for monitoring shrimp health and immunologic status. q

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“…The oxidative activity in molluscan hemocytes has been shown to be valuable in defining host-defen sive events (Adema et al, 1991;Anderson, 1994;Greger et al, 1995) and hemocytepathogen interactive modulation (Le Gall et al, 1991;Anderson et al, 1992b;Anderson, 1997, 1998).…”

mentioning

confidence: 99%

NADPH Oxidase-like Activity in Hemocytes of the Pacific Oyster Crassostrea gigas.

Takahashi

Mori

2000

Fish Pathol.

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Chemiluminescence analysis of the activity of Pecten maximua hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms

Cited by 47 publications

References 23 publications

Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): Current knowledge and future prospects

Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): Current knowledge and future prospects

Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus vannamei

NADPH Oxidase-like Activity in Hemocytes of the Pacific Oyster Crassostrea gigas.

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