Chemiluminescence and bioluminescence in chemical analysis

Abstract

Light accompanying a chemical reaction is known as chemiluminescence (CL). CL that occurs in a living system or is derived from one is known as bioluminescence

Determination of Montelukast Sodium by flow injection chemiluminescence

“…it was observed that the luminescence increase with the increasing of luminol concentration (the donor molecule to luminesce) and the recorded that, 5x10 -4 M was the best concentration to give it the highest intensity. according to the previous studies [8] the decrease may be happened in chemiluminescence intensity duo to the increasing in luminol concentration. That may be leading to increasing in the numbers of donor molecules as luminescence light in surrounding that leading to free collisions between the reacting molecules, some of it which entered in collision and producing excited molecules and the excess of it will reacting with molecules of reactants in surrounding, and luminol excited molecules causing loss in energy as thermal energynon radioactive -and thus chemiluminescence emission reduce.…”

“…Effect of ions flow rateEffect of flow rate for solution on the intensity of chemical luminescence emission was studied, after fixing all chemical variables, by changing the flow rate from 2 to 4 ml/min for hydrogen peroxide and luminol, and noticed that the increasing of emission intensity happened with increasing the flow rate at beginning 2-4 ml/min because the high flow rate leading to rapidity lightness segment that exit from measurement cell (there was a little to recording a signal), therefore, 3-5 ml/min was selected because it's consuming less of chemicals, and perform the desired purpose . degree measurement The dilution degree was calculated in presence and absent the ion exchanger columns because the flow injection technique is effects by dilution degree , all results that obtained from our study shown in table(8).Table (8) the obtained results from measurement of dilution degree average of the obtained results from measurment of dilution degree ( after placing the column of exchanger ion ) the obtained results from measurment of dilution degree (before placing the column of exchanger ion ) Standard curve to determination the Montelukast drug The relationship between the changing of drug concentration comparing with chemiluminescence intensity of magnisum ion at concentration 150 ppm, as shown infigure (9), it was noticed that there was increase in intensity of chemiluminescence due to functional role of druge. the linear equation was : Y=2535.2X + 657.32 , and detection limit was (0.0001M), correlation cofficient = 0.9054.…”

Inorganic and geological materials

“…Several general books and reviews on the analysis of ferrous alloys and ores have also been published (19,242,261,492,493), with particular emphasis on the analysis of raw materials, ores, slags, and metal powders by X-ray, emission, and nuclear methods (72,151,162,163,190,489,543). The analysis of steels and ferroalloys for trace elements has been reviewed (282,291,562).…”

Bioluminescence

“…The most frequent application is the ATP assay using firefly bioluminescence and this literature has been reviewed [64]. Other reviews of analytical applications are [65,71,72]. The Renilla bioluminescence system has been used to assay adenosine-Y-phosphate-5'phosphate with a sensitivity of lo-'* mo1 [72].…”