Chemiluminescence‐based aptasensors for various target analytes

Abstract

Aptamers (DNA or RNA) have complex three‐dimensional shapes that can bind to specific targets. Relative to antibodies, aptamers benefit from their low cost of production, easy chemical modification, high chemical stability, reproducibility, and low levels of immunogenicity and toxicity. However, the true value of aptamers lies in their simplicity by which these molecules can be engineered into sensors as bio‐recognition elements in diagnostics, drug discovery and therapy, environmental monitoring and food quality testing, etc. Many different types of techniques, such as optical, electrochemical, radiochemical and piezoelectronic methods, have been applied for the design of aptamer‐based methods, in which chemiluminescence (CL) detection techniques have become very popular in recent years. This review focuses on the recent advances in the development of aptamer‐based CL sensors for different target detection. We highlight specific examples that showcase the use of aptamers in practical applications, and provide the challenges and opportunities in this promising field.

An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk

“…However, the above methods need expensive equipment and experienced laboratory technicians, and the preparation required prior to a test is complicated and takes a long time. In recent years, electrochemical sensors have been considered to be one of the preferred technologies due to their low cost, simple operation, portability, fast response speed, high sensitivity, and wide application range [19][20][21][22].…”

Aptamer-Based Biosensors for Environmental Monitoring

“…Aptamers are particularly wellsuited for applications in environmental monitoring because they are chemically stable, easily chemically modified, relatively easy to synthesize, and biocompatible (Ruigrok et al, 2011). As such, researchers have previously been successful in using aptamers to build categorically diverse biosensors for the detection of a wide range of environmentally relevant analytes (Rapini and Marrazza, 2017;Cunha et al, 2018;Geleta et al, 2018;Mishra et al, 2018;Sun and Lu, 2018;Yan X. et al, 2018;Zhang et al, 2018e;Alkhamis et al, 2019;Moro et al, 2019;Verdian et al, 2019;Zhao Q. et al, 2019;Kudłak and Wieczerzak, 2020).…”

Advances in chemiluminescence and electrogenerated chemiluminescence based on silicon nanomaterials

“…To date, many classical CL and ECL systems involving luminol, lucigenin, peroxalate, 1,2‐dioxetanes and tris(bipyridine)ruthenium (II) ([Ru(bpy) 3 ] 2+ ) have been developed. [ 3–9 ] With rapid development of nanoscience and nanotechnology, nanomaterial‐based CL and ECL have also attracted considerable interest as an efficient way of improving CL/ECL intensity and designing novel CL/ECL sensors for ultrasensitive detection with the added benefits of low cost, high sensitivity, and good stability and reproducibility. [ 10–22 ]…”