Chemiluminescence (CL) in blood samples reflects leukocyte activation (LA) during anaphylactoid reactions in dogs

Wilhelms, O.-H.; L, Lipponer; W, Seilnacht

doi:10.1002/bio.1170050310

Cited by 3 publications

(2 citation statements)

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“…However, various authors use different CL methods to detect ROS produced by phagocytes of animals, which makes comparing their results difficult (6)(7)(8)(9)(10)(11)(12)(13)(14). Rats are among the most frequently used experimental animal models.…”

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confidence: 99%

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Pavelková

Kubala

2004

Luminescence

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Luminol-, isoluminol- or lucigenin-enhanced chemiluminescence (CL) was used to measure the production of reactive oxygen species by rat blood leukocytes. Opsonized zymosan (OZ), phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 (Ca-I) or N-formyl-Met-Leu-Phe (fMLP) were used as activators. The CL signal of isolated blood leukocytes decreased in rank order of luminol > isoluminol > lucigenin. The kinetic profiles of luminol- and isoluminol-enhanced CL were similar upon stimulation by each activator tested. The remarkably higher luminol and isoluminol CL responses were obtained after OZ stimulation when compared with other activators. However, when lucigenin was used, the PMA- and OZ-stimulated CL were comparable. The presence of plasma increased OZ-activated CL because of the enhanced phagocytosis of OZ. This was demonstrated by determining the phagocytosis of the fluorescent OZ using a flow cytometer. In contrast, the presence of plasma decreased PMA-activated CL, due to the antioxidant properties of plasma as determined by the CL method. As far as whole blood is concerned, only OZ activated luminol-enhanced CL was reliable. Blood volumes over 5 microL decreased CL activity due to the scavenging ability of erythrocytes. The results suggest that 0.5 microL whole blood is sufficient for routine luminol-enhanced CL analysis of whole blood oxidative burst in rats.

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confidence: 99%

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Pavelková

Kubala

2004

Luminescence

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“…Details of the methods have already been reported by us else where [14,19]. The most important technical details are summarised below.…”

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confidence: 99%

Intratracheal Application of Sephadex in Rats Leads to Massive Pulmonary Eosinophilia without Bronchial Hyperreactivity to Acetylcholine

Kubin¹,

Deschl²,

Linssen³

et al. 1992

Int Arch Allergy Immunol

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Fourteen Brown-Norway rats were pretreated with physiological saline (n = 7) or 500 μg Sephadex (n = 7) intratracheally. 24 h later, a bronchial provocation test was performed under pentobarbital anaesthesia using increasing doses of acetylcholine aerosol and the degree of bronchospasm was measured using a modified Konzett-Rössler method. Subsequently, leucocyte counts were determined in the bronchoalveolar lavage fluid (BALF), BALF cells were differentiated, and the chemiluminescence of the BALF leucocytes were measured. Finally, the lungs were removed and histologically examined. The cell count in the BALF was significantly (p < 0.05) increased in the animals pretreated with Sephadex compared to those in the saline group (mean value ± SEM: 0.38 ± 0.07 vs. 0.15 ± 0.02 × 10⁶/ml). This difference was also reflected in the chemiluminescence measurements (2.51 ± 0.53 vs. 0.20 ± 0.03 × 10⁶ counts/0.5 ml). In the Sephadex-treated animals there was also a significant increase in the absolute number of neutrophil (0.040 ± 0.010 vs. 0.011 ± 0.002 × 10⁶/ml) and, in particular, eosinophil granulocytes (0.188 ± 0.055 vs. 0.003 ± 0.001 × 10⁶/ml) in the total leucocytes of the BALF. Lung histology showed massive perialveolar and peribronchial oedema and granulomatous infiltrates, primarily with eosinophils, after intratracheal application of Sephadex; these findings were not observed in the saline group. None of these changes in the rats pretreated with Sephadex manifested themselves in increased bronchial reactivity to acetylcholine aerosol. It is uncertain if the Sephadex-induced increase in the eosinophil count is accompanied by an activation of this cell population, which appears to be of importance for the occurrence of bronchial hyperreactivity. It is clear from this animal model that the presence of inflammatory cells in the BALF and lung tissue of rats does not in itself result in increased reactivity of the airways.

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Chapter 7 Nonclinical evaluation of the potential for hypersensitivity

2004

Immunotoxicology of Drugs and Chemicals: An Experimental and Clinical Approach - Volume I: Principles and Methods of Immunotoxi

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Chemiluminescence (CL) in blood samples reflects leukocyte activation (LA) during anaphylactoid reactions in dogs

Cited by 3 publications

References 4 publications

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Intratracheal Application of Sephadex in Rats Leads to Massive Pulmonary Eosinophilia without Bronchial Hyperreactivity to Acetylcholine

Chapter 7 Nonclinical evaluation of the potential for hypersensitivity

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