Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Abstract

The present review examines the use of chemiluminescence detection to evaluate the course of free radical reactions in biological model systems. The application of the method is analyzed by using luminescent additives that enhance the luminescence thanks to a tripletsinglet transfer of the electron excitation energy from radical reaction products and its emission in the form of light with a high quantum yield; these additives are called chemiluminescence enhancers or activators. Examples of these substances are provided; differences between the so-called chemical and physical enhancers are described; coumarin derivatives, as the most promising chemiluminescence enhancers for studying lipid peroxidation, are considered in detail. The main problems related to the use of coumarin derivatives are defined, and possible ways of solving these problems are presented. Intrinsic chemiluminescence and the mechanism of luminescence accompanying biomolecule peroxidation are discussed in the first part of the review.

Features of the Kinetics of the Fenton Reaction

“…In Lin HC [12], when studying super weak luminescence in a mixture of Fenton with ortho-chlorophenol, the result of work [7] was confirmed that singlet oxygen is luminescent agent. In Romodin LA [13], chemiluminescence was considered in the study of free radical reactions, kinds of luminescent additives were discussed to increase the quantum yield of chemiluminescence. Research continues on Fenton like reactions [14] and new methods of chemiluminescence analysis in the Fenton reaction [15].…”