Chemiluminescence detection of telomere DNA in human cells on a membrane by using fluorescein-5-isothiocyanate-labeled primers

Yamasuji, Mutsumi; Shibata, Takayuki; Kabashima, Tsutomu; Kai, Masahiro

doi:10.1016/j.ab.2011.01.047

Cited by 25 publications

(13 citation statements)

References 13 publications

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“…Guanine, one of the four components -Adenine, Cytosine, Guanine, Thymine -of DNA aptamer, when reacting with 3,4,5-trimethoxylphenylglyoxal hydrate (TMPG), can produce a highenergy intermediate capable of emitting light by itself or transferring energy to fluorescent dye-conjugated with DNA aptamer based on the principle of intra chemiluminescence resonance energy transfer (Intra-CRET) as shown in Scheme 1 [11][12][13]. Light emitted from the reaction of DNA aptamer and TMPG in the presence of tetra-n-propylammonium hydroxide (TPA) and N,Ndimethylformamide (DMF) is called guanine chemiluminescence [11].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Khang¹,

Kim²,

Chung³

et al. 2016

Talanta

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Section: Introductionmentioning

confidence: 99%

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Khang¹,

Kim²,

Chung³

et al. 2016

Talanta

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“…Recently, Yamasuji et al (2011) observed bright chemiluminescence emitted in the presence of fluorescein-5-isothiocyanate (FITC) conjugated with G-rich single strand DNA. 1(c), FITC excited by dim chemiluminescence generated from the reaction between G-rich single strand DNA and TMPG emitted bright green light (Yamasuji et al, 2011). 1(c), FITC excited by dim chemiluminescence generated from the reaction between G-rich single strand DNA and TMPG emitted bright green light (Yamasuji et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Cho¹,

Park

Chong³

et al. 2014

Biosensors and Bioelectronics

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Cost-effective and sensitive aptasensor with guanine chemiluminescence detection capable of simply quantifying thrombin in human serum was developed using thrombin aptamer (TBA), one of the G-quadruplex DNA aptamers, without expensive nanoparticles and complicated procedures. Guanines of G-quadruplex TBA-conjugated carboxyfluorescein (6-FAM) bound with thrombin do not react with 3,4,5-trimethoxylphenylglyoxal (TMPG) in the presence of tetra-n-propylammonium hydroxide (TPA), whereas guanines of free TBA- and TBA-conjugated 6-FAM immobilized on the surface of graphene oxide rapidly react with TMPG to emit light. Thus, guanine chemiluminescence in 5% human serum with thrombin was lower than that without thrombin when TBA-conjugated 6-FAM was added in two samples and incubated for 20 min. In other words, the brightness of guanine chemiluminescence was quenched due to the formation of G-quadruplex TBA-conjugated 6-FAM bound with thrombin in a sample. High-energy intermediate, capable of emitting dim light by itself, formed from the reaction between guanines of TBA and TMPG in the presence of TPA, transfers energy to 6-FAM to emit bright light based on the principle of chemiluminescence energy transfer (CRET). G-quadruplex TBA aptasensor devised using the rapid interaction between TBA-conjugated 6-FAM and thrombin quantified trace levels of thrombin without complicated procedures. The limit of detection (LOD = background + 3 × standard deviation) of G-quadruplex TBA aptasensor with good linear calibration curve, accuracy, precision, and recovery was as low as 12.3 nM in 5% human serum. Using the technology reported in this research, we expect that various types of G-quadruplex DNA aptasensors capable of specifically sensing a target molecule such as ATP, HIV, ochratoxin, potassium ions, and thrombin can be developed.

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“…Chemiluminescence (CL), in which light is emitted from a chemical reaction, has attracted considerable research interest in recent years due to a wide calibration range, simple and rapid operation, the lack of background scattering light interference and the use of relatively inexpensive apparatus . More recently, CL has been used to investigate, for example, the organization of the organism, clinical drugs, environmental pollution, and so on.…”

Section: Introductionmentioning

confidence: 99%

Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO₄–rhodamine B chemiluminescence

Yang

Chen

2017

Luminescence

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The flow-injection chemiluminescence (FI-CL) behavior of a gold nanocluster (Au NC)-enhanced rhodamine B-KMnO system was studied under alkaline conditions for the first time. In the present study, the as-prepared bovine serum albumin-stabilized Au NCs showed excellent stability and reproducibility. The addition of trace levels of fluvoxamine maleate (Flu) led to an obvious decline in CL intensity in the rhodamine B-KMnO -Au NCs system, which could be used for quantitative detection of Flu. Under optimized conditions, the proposed CL system exhibited a favorable analytical performance for Flu determination in the range 2 to 100 μg ml . The detection limit for Flu measurement was 0.021 μg ml . Moreover, this newly developed system revealed outstanding selectivity for Flu detection when compared with a multitude of other species, such as the usual ions, uric acid and a section of hydroxy compounds. Additionally, CL spectra, UV-visible spectroscopes and fluorescence spectra were measured in order to determine the possible reaction mechanism. This approach could be used to detect Flu in human urine and human serum samples with the desired recoveries and could have promising application under physiological conditions.

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Chemiluminescence detection of telomere DNA in human cells on a membrane by using fluorescein-5-isothiocyanate-labeled primers

Cited by 25 publications

References 13 publications

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO₄–rhodamine B chemiluminescence

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Chemiluminescence detection of telomere DNA in human cells on a membrane by using fluorescein-5-isothiocyanate-labeled primers

Cited by 25 publications

References 13 publications

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO4–rhodamine B chemiluminescence

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Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO₄–rhodamine B chemiluminescence