Chemiluminescence - High Performance Liquid Chromatography of Phosphatidylcholine Hydroperoxide

Miyazawa, Teruo; Yasuda, K.; Fujimoto, Kenshiro

doi:10.1080/00032718708062941

Cited by 137 publications

(61 citation statements)

References 10 publications

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“…We used the CL-HPLC method of Miyazawa et al (22,23). Ten microliters of ECP solution (at a concentration of 0.05% or 0.1%) or 30% of ethanol as a vehicle was topically applied to a 3 cm 2 area on the inside of a human upper arm.…”

Section: Cl-hplc Of Human Skin Lipidsmentioning

confidence: 99%

Antioxidant Effects of Caesalpinia paraensis Extract on Human Skin Lipid Peroxidation

et al. 2003

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Section: Cl-hplc Of Human Skin Lipidsmentioning

confidence: 99%

Antioxidant Effects of Caesalpinia paraensis Extract on Human Skin Lipid Peroxidation

et al. 2003

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“…Several sensitive methods for detecting and identifying the hydroperoxides of three lipid groups have been reported. [13][14][15][16][17][18] However, the formation of regioisomeric hydroperoxides from each of the above-mentioned lipid group and their prosperity and decay has not yet been sufficiently clarified. To understand the lipid oxidation mechanism in tissue or blood, it would be desirable to clarify the possible regioisomers of each oxidized lipid group.…”

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confidence: 99%

Determination of Regioisomeric Hydroperoxides of Fatty Acid Cholesterol Esters Produced by Photosensitized Peroxidation Using HPLC

et al. 2000

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It has been widely known that lipid hydroperoxidation has a pathogenic effect for atherosclerosis, 1,2 and possibly contributes to both cancer and aging. [3][4][5][6][7] The oxidation of lipids proceeds via a free-radical chain mechanism. Singlet O2 is one of the reactive species for initiating the above oxidation process, and lipid hydroperoxides are believed to be produced as primary oxidative products. The hydroperoxidation of unsaturated fatty acids has also been reported to produce regioisomeric hydroperoxides by photosensitized singlet O2 oxidation or autooxidation. [8][9][10][11] Kritharides et al. showed the initial formation of lipid hydroperoxides by the oxidation of low-density lipoprotein (LDL) using HPLC analysis. 12 It is considered that the three lipid groups (cholesteryl esters, triglycelides and phospholipids) are to be oxidized in biological fluids. Several sensitive methods for detecting and identifying the hydroperoxides of three lipid groups have been reported. [13][14][15][16][17][18] However, the formation of regioisomeric hydroperoxides from each of the above-mentioned lipid group and their prosperity and decay has not yet been sufficiently clarified. To understand the lipid oxidation mechanism in tissue or blood, it would be desirable to clarify the possible regioisomers of each oxidized lipid group. The purpose of this work was to clarify the components of hydroperoxides produced from cholesteryl oleate (C18-1), cholesteryl linoleate (C18-2) and cholesteryl linolenate (C18-3) by photosensitized peroxidation. Experimental MaterialsOleic, linoleic and linolenic acids and their methyl esters were purchased from Kanto Chemical Co., and were purified by SiO2 column chromatography (hexane/ethyl acetate = 3/1 for acids and 20/1 for methyl esters, v/v) before use. The purities of the fatty acids and their methyl esters were over 95%; this was confirmed by 1 H-NMR analysis. They were also shown by GLC to be free from other isomers. Cholesterol was purchased from Wako Pure Chemical Inc., and used after SiO2 column chromatography (hexane/ethyl acetate = 5/1) and recrystallization. All of the solvents and reagents used were of analytical grade. InstrumentationHPLC was performed by the LC-6A system (Shimadzu Co.) including the Rheodyne 7125 injector, and the SPD-10A UV-VIS detector (Shimadzu Co.). An analytical column used was Mightysil Si 60 (4.5 × 250 mm, 5 µm, Kanto Chemical Co.); the mobile phase was a mixture of hexane/2-propanol (100/1 or 250/1, v/v). The flow rate was 1 ml/min and the temperature was ambient. The detection wavelengths were 210 and 233 nm. Mass spectra were taken using a JEOL Automass under the following conditions: column, DB-1, 30 m × 0.25 mm (J & W Co.); column temperature, 80˚C to 280˚C at a rate of 20 ˚C/min; carrier gas, helium (60 ml/min); ion source temperature, 250˚C; separator temperature, 250˚C; ionization energy, 70 eV.1 H-NMR was taken with a JEOL JNM EX-400 at 400 MHz with tetramethylsilane as an internal standard. Japan In order to examine the process of th...

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“…We measured phosphatidylcholine hydroperoxide in the liver, since this is a primary peroxidative product of phosphatidylcholine, which is the main lipidic component of hepatocellular membranes. It was measured using a CL-HPLC system as described by Miyazawa et al 26 with some modifications. Approximately 1 g of the frozen liver was homogenated in 2 mL of saline over an ice-water bath.…”

Section: Biochemical Studiesmentioning

confidence: 99%

Kupffer Cells Generate Superoxide Anions and Modulate Reperfusion Injury in Rat Livers After Cold Preservation

et al. 1997

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HIROSHI SHIBUYA, NOBUHIRO OHKOHCHI, KAZUHIKO SEYA, and SUSUMU SATOMI ies, we have demonstrated that the susceptibility of hepatoThis study was designed to determine the source of cytes to lipid peroxidation increases with the duration of cold reactive oxygen species (ROS) and whether Kupffer cells ischemia 7 and that neutrophils cause lipid peroxidation by modulate the injury of perfused rat liver after cold presreleasing superoxide in the perfused rat liver after cold preservation. The livers of male Lewis rats pretreated with ervation. 8 In addition, many investigators have demonschizophyllan glucan (SPG) (10 mg/kg, SPG group) or strated that scavengers for ROS and allopurinol, an inhibitor gadolinium chloride (5 mg/kg; Gd group) and untreated of xanthine oxidase, have a protective effect against ischemia/ rats (control group) were preserved in University of Wisreperfusion injury. 9,10 Another concern has been the identificonsin solution for 0, 12, and 24 hours at 4ЊC and then cation of the primary source of ROS. Several reports have perfused for 60 minutes with oxygenated Krebs-Henseimplicated xanthine oxidase as a major source of ROS during leit bicarbonate buffer. Real-time chemiluminescence reperfusion. 9,11 Other investigators have proposed neutro-(CL) of the liver during perfusion was measured using phils or Kupffer cells as the primary source of ROS. 12,13 Rea sensitive photomultiplier, and reperfusion injury was cent studies, however, have questioned whether the quantity assessed by measuring lipid peroxidation and lactate deof ROS generated during reperfusion is sufficient to cause hydrogenase release. CL intensity reached a peak within direct injury through lipid peroxidation. 14 Alternatively, ROS 5 minutes of reperfusion, and superoxide dismutase acts in signal transduction for subsequent injurious events. 15completely inhibited this CL in all groups. In the control Thus, the source of ROS and its impact on the pathogenesis group, the total CL intensity was high after 24 hours of of reperfusion injury remain controversial. preservation. It significantly (P õ .05 vs. control group)Little has been reported about direct measurement of ROS increased in the SPG group, while it decreased in the in a whole organ after cold preservation. Chemiluminescence Gd group after 12 and 24 hours of preservation. The total (CL) is used as a direct method for measuring ROS. A lumi-CL intensity decreased by 70% when diphenyliodonium nol-enhanced CL technique has been reported to be useful to chloride (100 mmol/L; an NADPH oxidase inhibitor) was determine ROS in the perfused rat liver during warm ischadded to the perfusate before preservation of untreated emia/reperfusion. 16,17 However, luminol CL is not a specific rats. Lipid peroxidation and lactate dehydrogenase reindicator of superoxide generation, since luminol would react lease significantly (P õ .05) deteriorated in the SPG with a wide variety of oxidants. 18 Recently, 2-methyl-6-[pgroup, while they ameliorated in the Gd group after 24 methoxyphenyl]-3,7-dihyd...

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Chemiluminescence - High Performance Liquid Chromatography of Phosphatidylcholine Hydroperoxide

Cited by 137 publications

References 10 publications

Antioxidant Effects of Caesalpinia paraensis Extract on Human Skin Lipid Peroxidation

Antioxidant Effects of Caesalpinia paraensis Extract on Human Skin Lipid Peroxidation

Determination of Regioisomeric Hydroperoxides of Fatty Acid Cholesterol Esters Produced by Photosensitized Peroxidation Using HPLC

Kupffer Cells Generate Superoxide Anions and Modulate Reperfusion Injury in Rat Livers After Cold Preservation

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