Chemiluminescence microflow injection analysis system on a chip for the determination of uric acid without enzyme

He, De‐Yong; Zhang, Zhujun; Huang, Ying; Hu, Yufei; Zhou, Houjiang; Chen, Dinglong

doi:10.1002/bio.847

Cited by 42 publications

(27 citation statements)

References 19 publications

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“…For uric acid determination, various techniques such as chemiluminescence 6,7 , fluorescence 8 , HPLC-mass spectrometry 9 , capillary electrophoresisamperometry 10 , electrochemistry 11,12 , colorimetry 13 and enzymatic test-kits have been reported. However, these methods are usually laborious, expensive, time-consuming and/or complex to perform.…”

mentioning

confidence: 99%

Amperometric carbon paste enzyme electrodes for uric acid determination with different mediators

Erden

Pekyardımcı

Kılıç

2011

Collect. Czech. Chem. Commun.

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In this study, two new amperometric carbon paste enzyme electrodes for determination of uric acid were developed. The carbon paste was prepared by mixing uricase enzyme, 1,4-benzoquinone or poly(vinylferrocene) (PVF) as a mediator, graphite powder, paraffin oil and then the paste was placed into cavity of a teflon electrode body. Determination of uric acid was performed by oxidation of enzymatically generated H 2 O 2 . The effects of enzyme loading, mediator amount, buffer type, pH, buffer concentration, working potential and temperature were investigated for both electrodes. The working range of the 1,4-benzoquinone modified enzyme electrode was 1.9 × 10 -8 -2.7 × 10 -3 M, detection limit 1.9 × 10 -8 M and response time 150 s. Optimum buffer type, pH, buffer concentration, working potential, temperature and amounts of enzyme and mediator for 1,4-benzoquinone modified enzyme electrode were found to be Tris, 8.0, 0.20 M, +0.25 V, 30°C, 2.0 Unit and 13%, respectively. The working range of the PVF modified enzyme electrode was 7.4 × 10 -8 -7.0 × 10 -3 M, detection limit 7.4 × 10 -8 M and response time 120 s. Optimum buffer type, pH, buffer concentration, working potential, temperature and amounts of enzyme and mediator for PVF modified enzyme electrode were found to be phosphate, 8.0, 0.05 M, +0.70 and +0.30 V, 40°C, 2.0 Unit and 10.9%, respectively. The repeatability, storage stability of the enzyme electrodes and interference effects were also investigated. Enzyme electrodes were used for determination of uric acid in serum samples and the results were in a good agreement with those obtained by commercial enzymatic kits.

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mentioning

confidence: 99%

Amperometric carbon paste enzyme electrodes for uric acid determination with different mediators

Erden

Pekyardımcı

Kılıç

2011

Collect. Czech. Chem. Commun.

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“…The values obtained ranged between 0.8 and 5.0 %. The estimated sampling frequency under the working conditions was about 15 h −1 , including three measurements and the corresponding cleaning steps, which is a relatively low value owing to the reverse mode used, although similar sample throughputs have been described in other μFI methods [10,11].…”

Section: Optimization Of Variablesmentioning

confidence: 82%

“…Most μFI-CL methods described involve the use of luminol or a ruthenium(II) complex. For instance, the luminol-ferricyanide CL system has been used for the determination of glucose [10] and uric acid [11] in human serum, and nitrite in food [12], obtaining detection limits of 18 μg mL −1 , 0.5 μg mL −1 , and 4 ng mL −1 , respectively, and a sample throughput between 12 and 30 h −1 . The CL reaction of peroxidisulphate with the complex of ruthenium(II) with 1,10-phenanthroline [13,14] or 2,2′-bipyridyle [15] has been mainly applied to the determination of therapeutic drugs in pharmaceutical formulations, reaching low detection limits, at the level of ng mL −1 , and high sample throughputs.…”

Section: Introductionmentioning

confidence: 99%

Determination of aminoglycoside antibiotics using an on-chip microfluidic device with chemiluminescence detection

2012

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We describe an on-chip microflow injection (μFI) approach for the determination of aminoglycoside antibiotics using chemiluminescence (CL) detection. The method is based on the inhibition of the Cu(II)-catalyzed CL reaction of luminol and hydrogen peroxide by the aminoglycosides due to the formation of a complex between the antibiotic and Cu(II). The main features of the method include small sample volumes and a fast response. Syringe pumps were used to insert the sample and the reagents into the microfluidic device. CL was collected using a fiber optic bundle connected to a luminescence detector. All instrumental, hydrodynamic and chemical variables involved in the system were optimized using neomycin as the aminoglycoside model. Inhibition is proportional to the concentration of the antibiotics. The dynamic ranges of the calibration graphs obtained for neomycin, streptomycin and amikacin are 0.3-3.3, 0.9-13.7, and 0.8-8.5 μmol L −1 , and the detection limits are 0.09, 0.28 and 0.24 μmol L −1 , respectively. The precision of the methods, expressed as relative standard deviation, is in the range from 0.8 to 5.0 %. The method was successfully applied to the determination of neomycin in water samples, with recoveries ranging from 80 to 120 %.

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“…Therefore, new materials and methodology are urgently needed for separation, monitoring, determination, and diagnosis of uric acid from/in urine and human serum. Traditional techniques such as chemiluminescence , fluorescent biosensor , spectrophotometry , ion chromatography , and electrochemical methods have been developed for the determination of uric acid concentration in biological samples. However, these methods are usually laborious, expensive, time‐consuming, and/or complex to perform.…”

Section: Introductionmentioning

confidence: 99%

Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres

Gong

Liu

et al. 2017

J. Sep. Sci.

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We aim to develop novel photoresponsive surface molecularly imprinted polymer (SIMP) microspheres, an SiO -SIMP, for the photocontrolled extraction of uric acid from biological samples. The SiO -SMIP was prepared on silica microspheres by surface polymerization and characterized by using scanning electron microscopy, transmission electron microscopy, FTIR spectroscopy, thermogravimetric analysis, nitrogen adsorption-desorption analysis, and UV-visible spectroscopy. The SiO -SMIP microspheres showed a photocontrolled uptake and release of uric acid in NaH PO buffer upon alternate irradiation at 365 and 440 nm. The SiO -SMIP microspheres were able to photocontrollably extract uric acid from complicated biological samples for concentration analysis with no significant interference encountered and it exhibited very good recognition ability and fast binding kinetics toward uric acid.

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Chemiluminescence microflow injection analysis system on a chip for the determination of uric acid without enzyme

Cited by 42 publications

References 19 publications

Amperometric carbon paste enzyme electrodes for uric acid determination with different mediators

Amperometric carbon paste enzyme electrodes for uric acid determination with different mediators

Determination of aminoglycoside antibiotics using an on-chip microfluidic device with chemiluminescence detection

Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres

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