Chemiluminescence of nicotinamide adenine dinucleotide reduced disodium salt—europium ions—hydrogen peroxide system

Elbanowski, M.; Paetz, Maria; Sławiński, Janusz; Ludwiczak, Ewa

doi:10.1016/1010-6030(91)85101-l

Cited by 4 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduced nicotinamide adenine nucleotide (NADH) molecule is another relevant biochemical compound, related to the nucleotides and extending this group [14]. As follows from a comparison of the reaction course, formation time and CL intensity of Eu(II)/ Eu(ΙII)-ΝΑDH-Η2O2 system under investigation and the Eu(II)/ Eu(IΙI)-ΑΤΡ-Η2O2 system, CL intensity is much higher and its duration longer in the case of ATP than NADH.…”

Section: Our Own Studies On the Applications Of The CL Methods With Lnmentioning

confidence: 99%

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

1993

View full text Add to dashboard Cite

•This article concerns problems related to chemiluminescence and briefly fluorescence, which will be discussed. It also gives a review of application of the chemiluminescent method in chemistry, biology and medicine, in the absence of 1anthanide ions and in their presence.PACS numbers: 78.60.PsIn this review the following important problems are covered: 1. Chemiluminescence (CL) as a phenomenon and a method of study. 2. The occurrence of CL in chemistry, biology and medicine (without the presence' of lanthanide ions, Ln(ΙIΙ)). 3. Importance of the lanthanide ions in the investigations discussed. 4. The role of the fluorescent method in the studies. 5. Our own studies on the applications of the CL method with Ln(ΙIΙ) ion as a spectroscopic probe. Chemiluminescence as a phenomenon and a method of studyChemiluminescence [1-3], similarly as bioluminescence, is still a very interesting phenomenon and a modern spectroscopic method used mainly in chemistry, biology and medicine. The discussed method may be applied in investigation of many problems in these fields.Chemiluminescence is an ultra-weak emission of light generated as a result of chemical reaction. As CL is a complicated process, it can be, in general, divided into two steps: the first -the excitation stage connected with generation of excited molecule identical with the excited product, and the second -the luminescence stage at which the excited molecule is deactivated to its ground state emitting a quantum of radiation. The first stage is of chemical nature, while the second - (993)

show abstract

Section: Our Own Studies On the Applications Of The CL Methods With Lnmentioning

confidence: 99%

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

1993

View full text Add to dashboard Cite

show abstract

“…Ιntroduction A chemiluminescence (CL) method is used to study both biological and inorganic compounds in systems containing lanthanide ions, mainly europium [1][2][3]. The basic reaction of the method is oxidation of Eu(II) to Eu(III) ions, occurring in complexes with ligand, through hydrogen peroxide and excitation of these ions, which then come down to the ground state emitting characteristic radiation [3].…”

mentioning

confidence: 99%

Influence of N₃^-Ions on Chemiluminescence of the Eu(II)/Eu(III)-H₂O₂System

et al. 1996

View full text Add to dashboard Cite

The basic system of chemiluminescence investigations of relevant biological and inorganic compounds consists of Εu(II)/Εu(III)-Η2O2 . In these studies the increase in Eu(III) ion emission intensity usually results from an effective complexation reaction. In the present study, the Ν3 ions are used as a ligand. The increase in the chemiluminescence intensity of the Εu(III) ion, the pH influence (in the range of 4.5-7.5) on the chemiluminescence intensity of the Eu(lII) ion, as well as the quenching of the 5D0 excited state of the Εu(IIl) ion are observed due to Ν3 complexation. Taking into account the well-known Fenton system [Fe(II)/Fe(III)-Η2O2], containing additionally the Ν3, Eu(lII) or Sm(llI) ions, we found that the Εu(III) or Sm(III) ions were excited as a result of energy transfer process. A mechanism of the studied reactions is proposed.PACS numbers: 78.60.Ps, 78.55.-m 1. Ιntroduction A chemiluminescence (CL) method is used to study both biological and inorganic compounds in systems containing lanthanide ions, mainly europium [1][2][3]. The basic reaction of the method is oxidation of Eu(II) to Eu(III) ions, occurring in complexes with ligand, through hydrogen peroxide and excitation of these ions, which then come down to the ground state emitting characteristic radiation [3]. Products of simultaneous reactions of H 2 O 2 decomposition and radicals recombination are excited dimoles of a singlet state 1O2 [4, 5], whose influence on the level of CL may by determined using quenchers [6]. In literature the often reported quencher of an excited state 1 O2 are azide ions Ν . So far, the mechanism of 1 O2 emission quenching by Ν3 ions has not been well recognized. While Harbour' and Issler claim that the process of 1 O2 2 quenching proceeds through formation of charge-transfer complex [7], Haag and Mill prove that the dominant reaction in this process is typical physical quenching [8].In our studies incorporation of azide ions, Ν3 , into the Eu(ΙΙ)/Eu(IΙI)-H 2 O 2 system, brings about a strong increase in chemiluminescence emission characteristic of Εu(III) ions. On the basis of both chemiluminescence and fluorescence studies, where particular emphasis was paid to the measurements of a lifetime of 5 D0 Εu(III) ions, a mechanism of chemiluminescence process was proposed.(101)

show abstract

Chemiluminescence of systems containing lanthanide ions

Elbanowski

Mąkowska

Staniński

et al. 2000

Journal of Photochemistry and Photobiology A: Chemistry

View full text Add to dashboard Cite

Chemiluminescence of nicotinamide adenine dinucleotide reduced disodium salt—europium ions—hydrogen peroxide system

Cited by 4 publications

References 16 publications

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

Influence of N₃^-Ions on Chemiluminescence of the Eu(II)/Eu(III)-H₂O₂System

Chemiluminescence of systems containing lanthanide ions

Contact Info

Product

Resources

About

Chemiluminescence of nicotinamide adenine dinucleotide reduced disodium salt—europium ions—hydrogen peroxide system

Cited by 4 publications

References 16 publications

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

Chemiluminescence Used in Biochemical Investigations. An Application of the Lanthanide Ions as a Chemiluminescent Probe

Influence of N3-Ions on Chemiluminescence of the Eu(II)/Eu(III)-H2O2System

Chemiluminescence of systems containing lanthanide ions

Contact Info

Product

Resources

About

Influence of N₃^-Ions on Chemiluminescence of the Eu(II)/Eu(III)-H₂O₂System