Chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients

Abstract

Polymorphonuclear leukocytes (PMN's) constitute a primary host resistance factor against infection. This study investigated the chemiluminescent (CL) response of peripheral blood PMN's isolated from human subjects with adult periodontitis. 32 subjects were categorized on the basis of age and periodontal disease status into 4 equal groups--young healthy, young diseased, old healthy and old diseased. PMN CL was stimulated using heat-killed, serum-opsonized Fusobacterium nucleatum--a specific periodontopathic gram-negative anaerobe, and Escherichia coli as a gram-negative control organism. The results showed a statistically significant enhancement (p less than 0.05) in the CL response, which was cell associated, in the young diseased subjects. This was not seen in the old subjects (p greater than 0.05), suggesting that in periodontal disease in young subjects the peripheral blood PMNs may be in a metabolically activated state. There was nevertheless a degree of variability between individual subjects within each of the 4 clinical groups.

Oxidative Injury and Inflammatory Periodontal Diseases : the Challenge of Anti-Oxidants to Free Radicals and Reactive Oxygen Species

“…PMN from both PLS patients and controls produced similar amounts of 02'-when stimulated with opsonized bacteria or PMA, but both resting PMN and PMN stimulated with polyhistidine from the PLS patients released larger amounts of 02' than PMN from the controls (Bimstein et al, 1990). In AP, only a slight increase in CL was observed (Whyte et al, 1989), while there were minor differences (Zafiropoulos et al, 1991), no differences (Mouynet et al, 1994), or a CL decrease (Gomez et al, 1994) among healthy subjects or patients with gingivitis, juvenile periodontitis, or AP PMN from AP patients have higher CL after Fcgreceptor stimulation than do those from healthy subjects (Gustafsson and Asman, 1996). In RPP patients was found a lowering of CL (Zafiropoulos et al, 1988), and only minor differences in the CL responses between PE patients and healthy controls were found in terms of the oxidative metabolism of PMN (Zafiropoulos et al, 1991).…”

Antimicrobial activity of honokiol and magnolol isolated from Magnolia officinalis

“…It is likely that the role of reactive oxygen molecules is common to both bacterial and host mediated pathways of tissue damage. The radicals have been implicated as important pathologic mediators in many clinical disorders (Slater and Cheeseman, 1987), and more recently periodontal diseases (Whyte et al, 1989;Shapira et al, 1991;Kimura et al, 1993).…”

Cigarette smoking as an aggravating factor in inflammatory tissue-destructive diseases. Increase in tumor necrosis Factor-α priming of peripheral neutrophils measured as generation of oxygen radicals

“…Its potential tissue-damaging capacity, as described by Weiss [4], involves simultaneous release of free oxygen radicals and proteases. Neutrophil hyperreactivity may be an important mechanism of tissue destruction in periodontitis [5,6], chronic obstructive pulmonary diseases [7,8], Crohn's disease [9,10], and atherosclerosis [11,12].…”