Chemiluminescence of soybean saponins in the presence of active oxygen species

Yoshiki, Yumiko; Kinumi, Masaya; Kahara, Takashi; Okubo, Kimihiro

doi:10.1016/0168-9452(96)04375-0

Cited by 19 publications

(2 citation statements)

References 13 publications

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“…In addition to the synergistic effect contributed by the various isoflavones in the soybean cake extract, the presence of some other functional components cannot be ignored. Several reports have revealed that soyasapogenol B was effective in scavenging reactive oxygen ( , ) and inhibiting oxidative damage caused by reactive oxygen when administered to rats (). Thus, the antioxidant activity of the acetylglucoside fraction can be mainly attributed to the presence of a large amount of soyasapogenol B ( Table ).…”

Section: Resultsmentioning

confidence: 99%

Functional Components in Soybean Cake and Their Effects on Antioxidant Activity

Kao

Chen

2006

J. Agric. Food Chem.

171

121

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The antioxidant activities of four fractions of isoflavones from soybean cake were evaluated and compared with those of ISO-1 and ISO-2 fractions, five isoflavone standards, and mixtures of two or four isoflavone standards, as well as four commercial antioxidants, using DPPH, TEAC, reducing power, metal ion chelating, conjugated diene, and TBARS assays. Both malonylglucoside and glucoside fractions were isolated using preparative chromatography with Diaion HP-20 as adsorbent, whereas acetylglucoside and aglycone fractions were separated with silica gel as adsorbent. The other two fractions, ISO-1 and ISO-2, were soybean cake extracts containing 12 isoflavones for the former and a combination of 4 fractions for the latter. Both acetylglucoside and ISO-1 fractions exhibited the highest efficiency in scavenging DPPH free radicals, whereas all six fractions were effective in inhibiting conjugated diene formation. However, a low reducing power was observed for all six fractions and isoflavone standards. The aglycone fraction and genistein standard showed a pronounced increase of TEAC value and a moderate decrease of TBARs value. For chelating metal ions, both ISO-1 and ISO-2 fractions were the most efficient. Overall, the isoflavone fractions showed a better antioxidant activity than the isoflavone standards, probably caused by the presence of some other functional components such as saponin, flavonoid, and phenolic compounds in soybean cake.

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Section: Resultsmentioning

confidence: 99%

Functional Components in Soybean Cake and Their Effects on Antioxidant Activity

Kao

Chen

2006

J. Agric. Food Chem.

171

121

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“…항산화에 중요한 전사인자인 Nrf2 로 조절 되는 SOD-1과 SOD-2, SOD-3의 발현 정도를 조사한 결과 spermidine는 전사인자인 Nrf2와는 독립적으로 SOD의 발현을 증가시키는 것으로 나타났는데, 이는 사람 피부섬유아 세포에서 spermidine은 적정농도에서 SOD-1, SOD-2 및 SOD-3의 발현을 증가시켜 항산화 효과를 나타낼 수 있다는 것을 암시하고 있다. 이전의 연구에서도 spermidine이 항산화 효소에 영향을 줄 수 있다고 보고하였는데, 이러한 결과는 본 연구결과와 상충된다 [38]. 그러므로 이러한 작용 기전에 의하 여 spermidine이 산화적 스트레스에 의한 질병을 예방 할 수 있는 하나의 근거 될 수 있다.…”

Section: Dpph Radical Assayunclassified

Inhibitory Effect of Spermidine with Antioxidant Activity on Oxidative Stress in Human Dermal Fibroblasts

Park¹,

Kim²

2011

Journal of Life Science

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Spermidine is a ubiquitous polycation that is synthesized from putrescine, which serves as a precursor of spermine. In recent years, spermidine was found to be a polyamine that plays an important role in longevity. Reactive oxygen species (ROS) such as hydroxyl radical, superoxide and hydrogen peroxide have been shown to be involved in various pathogenic processes as well as aging. The direct scavenging effect of spermidine on DPPH radical, H2O2 and hydroxyl radical, and its protective effect against DNA oxidation related to oxidative stress were evaluated in vitro. It was observed that spermidine exhibits scavenging activities on DPPH radical and H2O2 above 500 μM. Spermidine was especially effective in exerting a scavenging activity on hydroxyl radical. In addition, spermidine at 1000 μM showed a clear protective effect against DNA oxidation. Furthermore, the expression level of antioxidant enzymes such as superoxide dismutase in humam dermal fibroblasts increased in the presence of spermidine compared with blank group. These results suggest that spermidine can be used as an antioxidant to prevent ROS-related diseases including inflammation, cancer and aging.

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