Chemiluminescence Response of Human Phagocytes against IgG‐Opsonized Staphylococci with and without Protein A Activities

Yamada, Sakuo

doi:10.1111/j.1348-0421.1990.tb01005.x

Cited by 4 publications

(7 citation statements)

References 21 publications

(22 reference statements)

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“…We previously reported that IgG bound to protein A via its Fc portion had no significant effect on the phagocytic CL response (26). However, we did not obtain any clear evidence for any effect of protein A on the interaction between the bacteria and phagocytes without opsonins.…”

Section: Discussioncontrasting

confidence: 91%

“…Hemagglutination (HA) test. Protein A activity was quantitatively assayed by a previously reported method (22,(24)(25)(26). Briefly, two-fold serial dilutions (100 pl.…”

Section: Methodsmentioning

confidence: 99%

“…After two washings, the bacterial cells were counted in a Coulter Counter (ZM, Coulter Electronics, Inc., Hialeah, Fla., U.S.A.) and adjusted to 1 x 109 cells/ml with minimum essential medium (MEM) (Nissui) containing 10 mm HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid). The phagocyte suspension containing 5 x 106/m1 of polymorphonuclear leukocytes (PMN), on the other hand, was prepared in a previously reported manner (26). One hundred microliters of 0.2 mm luminol solution (Labo Science, Tokyo) was transferred into a polystyrene cuvette (Labo Science) and then 100 u1 of the phagocyte suspension was added.…”

mentioning

confidence: 99%

“…Protein A activity was quantitatively assayed by a previously reported method (22,(24)(25)(26) each) of the bacterial suspension in Dulbecco's phosphate-buffered saline (PBS) were placed in the wells of an agglutination tray (Tomy Seiko, Co., Tokyo) and 100 ill of a suspension of sheep red blood cells sensitized with rabbit antiserum was added to each well. The tray was incubated for 3 hr at room temperature.…”

mentioning

confidence: 99%

“…The magnitude of the CL response was scored by the peak height and total integral as the relative light units (R.L.U.) (26).…”

mentioning

confidence: 99%

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Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Yamada

Matsumoto

1990

Microbiology and Immunology

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To assess the surface properties of Staphylococcus aureus affecting the response of human phagocytes, the effects of the organisms with different surface properties on the chemiluminescence (CL) response of human phagocytes were examined. The magnitude of the phagocytic CL response to hydrophobic strains was significantly greater than that to hydrophilic strains, while no significant difference in the CL response was seen between protein A-deficient strains and their parent strains.The CL response to the hydrophilic organisms prepared from a hydrophobic strain by trypsin treatment decreased significantly.These results suggest that the phagocytic CL response to staphylococci depends on the hydrophobicity of the surface, but not on the presence of protein A. Two protein A-deficient strains which were isolated from protein A-positive strains showed identical hydrophobicity with their parent strains. All of the hydrophilic strains isolated from hydrophobic strains possessed protein A identical to that of their parent strains.Moreover, a hydrophilic strain could be isolated from a protein A-deficient, hydrophobic strain. These results strongly suggest that protein A is not solely responsible for the surface hydrophobicity of S. aureus.

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Section: Discussioncontrasting

confidence: 91%

“…Hemagglutination (HA) test. Protein A activity was quantitatively assayed by a previously reported method (22,(24)(25)(26). Briefly, two-fold serial dilutions (100 pl.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The magnitude of the CL response was scored by the peak height and total integral as the relative light units (R.L.U.) (26).…”

mentioning

confidence: 99%

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Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Yamada

Matsumoto

1990

Microbiology and Immunology

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Effect of Gold Nanoparticles on Production of Reactive Oxygen Species by Human Peripheral Blood Leukocytes Stimulated with Opsonized Zymosan

et al. 2013

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We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm.

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Enhancing effect of protein A on the interaction between opsonized corneocytes and neutrophils in Staphylococcal infection

Kato

Terada

Hara

et al. 1993

Experimental Dermatology

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Formation of subcorneal pustules characterizes skin lesions infected by Staphylococcus aureus. To elucidate the mechanism underlying the subcorneal pustule formation as well as that of anti-bacterial host defence, we studied the effect of staphylococcal protein A on the interaction between the stratum corneum (SC) and neutrophils. We found that protein A significantly promoted opsonized SC-induced chemiluminescence (CL) in neutrophils. This was specific to SC because no enhancement was observed with opsonized zymosan. It took place even with the serum obtained from a patient with agammaglobulinemia, ruling out the possibility of the involvement of Fc-receptors of neutrophils in this phenomenon. Microscopic observation of such SC revealed an increase in the number of neutrophils adhering to the surface of the protein A-coated corneocytes. Ultrastructural observation showed a distinct deformation of the neutrophils adhering to the surface of the corneocytes, suggesting that they are in an activated state. Such an enhanced interaction between protein A-attached SC and neutrophils seems to play an important role in the host defence mechanism against the invading S. aureus and in the production of the characteristic pustules by the neutrophil-mediated damage of the surrounding epidermal tissue.

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Chemiluminescence Response of Human Phagocytes against IgG‐Opsonized Staphylococci with and without Protein A Activities

Cited by 4 publications

References 21 publications

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Effect of Gold Nanoparticles on Production of Reactive Oxygen Species by Human Peripheral Blood Leukocytes Stimulated with Opsonized Zymosan

Enhancing effect of protein A on the interaction between opsonized corneocytes and neutrophils in Staphylococcal infection

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