Chemiluminescence sensor for sulfonylurea herbicide using molecular imprinted microspheres as recognition element

Abstract

Uniform molecular imprinting microspheres were prepared using precipitation polymerization with thifensulfuron-methyl (TFM) as template, acrylamide as functional monomer and ethylene glycol dimethacrylate as cross-linker. TFM could be selectively adsorbed on the molecularly imprinted polymers (MIPs) matrix through the hydrogen bonding interaction and the adsorbed TFM could be sensed by its strikingly enhancing effect on the weak chemiluminescence (CL) reaction between luminol and hydrogen peroxide. On this basis, a novel CL sensor for the determination of TFM using MIPs as recognition elements was established. The logarithm of net CL intensity (ΔI) is linearly proportional to the logarithm of TFM concentration (C) in the range from 1.0 × 10(-9) to 5.0 × 10(-5) mol L(-1) with a detection limit of 8.3 × 10(-10) mol L(-1) (3σ). The results demonstrated that the MIP-CL sensor was reversible and reusable and that it could strikingly improve the selectivity and sensitivity of CL analysis. Furthermore, it is suggested that the CL enhancement of luminol-H(2)O(2) by TFM might be ascribed to the enhancement effect of CO(2), which came from TFM hydrolysis in basic medium.

Design and application of novel molecular imprinting fluorescent sensors

“…Yang 等 [95] 制备出基于二茂铁件系的分子印迹荧光传 感器, 可识别活细胞内的 Hg 2+ , 如图 5(a)所示. 本课 题组 [96] NBD 是一种疏水的绿色荧光染料, 刘艳等 [97] 采 用 一 步 聚 合 制 备 了 以 疏 水 的 聚 甲 基 丙 烯 酸 甲 酯 (PMMA)为核、亲水性的聚电解质支化聚乙烯亚胺 http://engine.scichina.com/doi/10.1360/N032016-00079 图 5 (a) 基于二茂铁体系的荧光传感器识别活性胞内 Hg 2+ [95] ; (b) 基于罗丹明的 L-Cys 分子印迹荧光检测机制 [96] 此外, 将 MIPs 与 CL 成像系统结合, 可实现对组 图 6 基于掺杂染料聚合物核壳纳米粒子的 Hg 2+ 比率型荧光检测传感器 [97] (网络版彩图) 图 7 (a) 基于化学发光的分子印迹流式传感器 [101] ; (b) 检测特布他林的微流控传感器芯片示意图 [103] http://engine.scichina.com/doi/10.1360/N032016-00079 织、细胞中特定物质的空间分布、宏观或微观样品中 的生化反应过程及生物体内的生物学或病理学过程 进行实时和在线评估 [104,105] .…”

A molecularly imprinted polymer based chemiluminescence array sensor for one‐step determination of phenothiazines and benzodiazepines in pig urine

“…In this method, the co‐existed interferents can be eliminated by the MIP effectively, so the method selectivity is highly improved. Therefore, the MIP‐CL method has been used to analyze many analytes, including PZs …”

Magnetic-luminescent bifunctional nanosensors

“…[39][40][41][42][43][44][45][46][47][48] Despite having been used for chemical or biological analysis, the MIP materials were usually used as solid-phase extraction cartridges only for concentrating and purifying the analytes. [49][50][51][52] In most cases, the extracted templates have to be removed from the MIP recognition sites, and then quantified with complicated and expensive instruments including GC-MS, HPLC and LC-MS. [52][53][54] To simplify the analysis process, coupled with the chemluminescence, [55][56][57] MIP technology has been successfully applied to the detection of organophosphate pesticides. Meanwhile, the photoluminescence [58][59][60][61][62][63] of organic dyes and quantum dots has also been integrated with the MIP technology.…”