Chemiluminescence study of the contents of the products of Cu2+-induced lipid peroxidation in different fractions of human blood lipoproteins

Abstract

It is demonstrated that the content of the primary products of lipid peroxidation reaches the maximum after about 1-h incubation with Cu 2 § and then declines. At a Cu 2 § concentration of about 10=15 ~tM, the content of lipid peroxidation products is maximal; it does not rise with a further increase in the Cu 2. concentration. Comparison of the kinetics of lipid peroxidation in different lipoprotein fractions shows that low density lipoproteins are much more strongly oxidized than high density lipoproteins. A strong positive correlation between the amplitude of the chemiluminescence burst and the diene conjugate content is established in 79 independent measurements.