Chemiluminescent Detection with Horseradish Peroxidase and Luminol

Heindl, Dieter; Josel, Hans‐Peter

doi:10.1007/978-3-642-57206-7_18

Cited by 2 publications

(2 citation statements)

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“…The luminescence intensity of the luminol oxidation product is proportional to the concentration of free radicals, which depends on the activity of the enzyme-like inorganic material. This method of analysis makes it possible to determine the enzyme-like activity of nanomaterials with high analytical precision [59][60][61][62][63][64][65].…”

Section: Enzyme-like Activity Of Ceo 2 Sols Towards H 2 O 2 Decomposi...mentioning

confidence: 99%

Peroxidase-like Activity of CeO2 Nanozymes: Particle Size and Chemical Environment Matter

et al. 2023

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The enzyme-like activity of metal oxide nanoparticles is governed by a number of factors, including their size, shape, surface chemistry and substrate affinity. For CeO2 nanoparticles, one of the most prominent inorganic nanozymes that have diverse enzymatic activities, the size effect remains poorly understood. The low-temperature hydrothermal treatment of ceric ammonium nitrate aqueous solutions made it possible to obtain CeO2 aqueous sols with different particle sizes (2.5, 2.8, 3.9 and 5.1 nm). The peroxidase-like activity of ceria nanoparticles was assessed using the chemiluminescent method in different biologically relevant buffer solutions with an identical pH value (phosphate buffer and Tris-HCl buffer, pH of 7.4). In the phosphate buffer, doubling CeO2 nanoparticles’ size resulted in a two-fold increase in their peroxidase-like activity. The opposite effect was observed for the enzymatic activity of CeO2 nanoparticles in the phosphate-free Tris-HCl buffer. The possible reasons for the differences in CeO2 enzyme-like activity are discussed.

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Section: Enzyme-like Activity Of Ceo 2 Sols Towards H 2 O 2 Decomposi...mentioning

confidence: 99%

Peroxidase-like Activity of CeO2 Nanozymes: Particle Size and Chemical Environment Matter

et al. 2023

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“…A chemiluminescence measurement combining HRP with luminol as the hybrid label has been reported by Tobos et al 112 Luminol is the substrate for HRP that specifically recognizes and catalyzes its transformation in the presence of H 2 O 2 to give a luminescence maximum at a wavelength of 430 nm, which can be visually detected. 113 Another hybrid label of HRP with AuNP as an indicator and enhancer, respectively, has been reported for an electrochemical Cu 2+ sensor. 114 Multiple signal amplification was realized in the work by many HRP molecules labeled AuNP to catalyze H 2 O 2 degradation due to Cu 2+ -induced click conjugation for environmental monitoring.…”

Section: Hybrid Labelsmentioning

confidence: 99%